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We narrowed to 276 results for: aav

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Showing: 251 - 276 of 276 results

  1. Deep Dive: qPCR

    Type
    Blog Post
    ...resources on the Addgene blog Digital Droplet PCR for AAV QuantificationPlasmid Cloning by PCR...

  3. Optogenetics Guide

    Type
    Guide
    ...also offer ready-to-use AAV preparations of many optogenetics plasmids and AAV Packaged on Request for...

  4. Deisseroth INTRSECT Collection

    Type
    Collection
    ...targeting strategy that allows adeno-associated virus (AAV)-borne payloads to be expressed in cells based on... Items 55636 pAAV-EF1a-Cre None Yes 55632 pAAV-Ef1a-mCherry-IRES-Cre None Yes 55637 pAAV-EF1a-Flpo None...None Yes 55634 pAAV-EF1a-mCherry-IRES-Flpo None Yes 55638 pAAV-EF1a-vCre None Yes 55635 pAAV-EF1a-sCre None...Items 55641 pAAV-Ef1a-fDIO EYFP Flp Yes 55640 pAAV-Ef1a-dDIO hChR2(H134R)-EYFP Dre No 55639 pAAV-Ef1a-fDIO...55650 pAAV-hSyn Con/Fon EYFP Cre AND Flp Yes 231926 pAAV-Ef1a-Con/Fon-EYFP Cre AND Flp No 55651 pAAV-hSyn...137134 pAAV-Ef1a-Coff/Fon-mCherry Flp AND NOT Cre Yes 137135 pAAV-Ef1a-oScarlet None Yes 137136 pAAV-Ef1a-Con... 137125 pAAV-Ef1a-sRGECO None Yes 137126 pAAV-Ef1a-Con/Fon-sRGECO Cre AND Flp Yes 137127 pAAV-Ef1a-Con...

  5. Plasmids for Stem Cell Research

    Type
    Collection
    ...Jun 6. Buganim Glial Cells Retinal Ganglion Cells AAV/CRISPR Mouse Glia-to-Neuron Conversion by CRISPR-...

  6. CRISPR Guide

    Type
    Guide
    ...efficiently packaged into adeno-associated viruses (AAVs) . Other natural Cas orthologs include Hsp1Cas9 ...Mammalian CRISPR libraries have also been created in AAV backbones for in vivo experiments and in a retroviral...capabilities but are small enough to be packaged in AAV particles. Cas13 fusions have also been used in in...

  7. Molecular Biology Reference

    Type
    Guide
    ...particles (most commonly lentiviral, retroviral, AAV, or adenoviral particles) that can infect your target...

  8. CRISPR Guide

    Type
    Collection
    ...efficiently packaged into adeno-associated viruses (AAVs) . Other natural Cas orthologs include Hsp1Cas9 ...Mammalian CRISPR libraries have also been created in AAV backbones for in vivo experiments and in a retroviral...capabilities but are small enough to be packaged in AAV particles. Cas13 fusions have also been used in in...

  9. Genetic Code Expansion

    Type
    Collection
    ...4xPyOtR-ITR-GFP PylRS M. barkeri Azidolysine (AzK) Mammalian, AAV TAG Abhishek Chatterjee 241290 pANAP-P2A-eRF(E55D...

  10. Our New Antibody Mascot is....

    Type
    Blog Post
    ...  Last month, we introduced you to Blugene and Aavery's newest friend, our Addgene antibody mascot, here...on Feb. 24. Stay tuned!    Original Blugene and Aavery would like your attention, please, as they introduce...AntibodyMakesThree on Twitter. As with Blugene and Aavery, we’re looking for a gender neutral name (and it...

  11. General Transfection

    Type
    Protocol
    ...of HEK293T cells optimized for viral production (AAVpro or Lenti-X), but any HEK293T cell line should work...

  12. Typing CRISPR Systems

    Type
    Blog Post
    ...362(6416), 839–842. https://doi.org/10.1126/science.aav4294 Hu, C., Myers, M. T., Zhou, X., Hou, Z., Lozen... 363(6422), 88–91. https://doi.org/10.1126/science.aav7271 Yoshimi, K., & Mashimo, T. (2022b). Genome ...

  13. Let There Be LITE Plasmids

    Type
    Blog Post
    ...offers a tip: save yourself some time by making an AAV1 supernatant. (The details can be found here.) So...

  16. Hot Plasmids - October 2022

    Type
    Blog Post
    ...available as Cre-dependent, EF1ɑ-driven viral vectors (AAV1) - and you can also find several other constructs...

  20. Bioinformatics at Addgene

    Type
    Blog Post
    ...SeqwellHow to Keep a Lab Notebook for Bioinformatics AnalysesAAV Vector Quality Control: Going the Extra Mile ...

  21. Neurodegeneration Plasmid Collection

    Type
    Collection
    ... 243760 AAV-CAG-GRN-HA GRN HA CAG Frontotemporal dementia (FTD) Andrew Arrant 244272 pAAVLINK-EF1alpha-HA...-FP Plasmid Type Mammalian, non-viral Lentiviral AAV Retroviral Bacterial Yeast Other Promoter CMV T7 ... ALS, motor neuron disease Trina Schroer 75437 p-AAV-sh[SNCA] SNCA U6 Parkinson's Edward Burton 75637 ...Parkinson's Hilal Lashuel 36055 pAAV asyn WT SNCA CMV Parkinson's Hilal Lashuel 36056 pAAV asyn S87A SNCA CMV Parkinson's...Hilal Lashuel 36066 pAAV asyn S129A SNCA CMV Parkinson's Patrick Aebischer 36067 pAAV asyn S129G SNCA CMV...Hilal Lashuel 36068 pAAV asyn S129E SNCA CMV Parkinson's Hilal Lashuel 36069 pAAV asyn S129D SNCA CMV ...Aebischer 36070 pAAV asyn S87A/S129A SNCA CMV Parkinson's Patrick Aebischer 36071 pAAV asyn S87D/S129A...

  22. CRISPR Plasmids - Tagging

    Type
    Collection
    ...targeting the AAVS1 locus. Doyon Tagging Plasmid: 3xFLAG-2xSTREP Construct for integrating at the AAVS1 "safe ...cloned directly into this vector and targeted to the AAVS1 genomic safe harbor locus using untagged SpCas9 ...Addgene 41815 or #44719 ) in combination with gRNA_AAVS1-T2 (Addgene #41818) or using an all in one vector...vector from the Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_T2 (Addgene #79888) , which expresses an untagged...safe harbor" locus: eSpCas9(1.1)_No_FLAG_AAVS1_T2 Doyon Lab TAP Tagging Protocol 97.2 KB Yamamoto PITCh Tagging...

  23. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Tight junction protein ZO-1 Tight junctions 91565 AAVS1-mEGFP AICSDP-35 mEGFP NA Cytoplasm 101781 CETN2-...mEGFP Ras-related protein Rab-5A Endosomes 107580 AAVS1-mTagRFPT-CAAX AICSDP-42 mTagRFPT CAAX domain of ...AICSDP-29 mTagRFPT Lamin B1 Nuclear envelope 114404 AAVS1-mEGFP (PGK) AICSDP-36 mEGFP NA Cytoplasm 114405 ...
Showing: 251 - 276 of 276 results