Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 771 results for: Dos;

Showing: 101 - 120 of 771 results

  1. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ...they do it.    Finding Conflict in Science  In all science communication, what you are doing is telling...they’re firmly part of the story to the scientists doing the work.  And while these pieces aren't always ...of my story” and that’s a very valid critique! But do you remember earlier when I said that even when we...t that sense of curiosity and wonder why you’re doing and sharing science in the first place?   References...

  2. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...that we are doing just that. Do you like the direction the blog has taken over the past year? Do you think... 7. Career Coaching for Scientists: Why and Where Do I Find One? If you need help making a transition...Addgenie Joanne Kamens explains what career coaches do and offers up some career coach suggestions. 8. ...

  3. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ...to the principle that you can't motivate people to do things, you can only demotivate them. The primary...less a really good one). While getting your PhD or doing a postdoc few science trainees will have opportunities...hard and most people have some learning they need to do before they can be good at it. Like any other skill...freedom and diversity built into their jobs Chance to do/learn new things, diversity of responsibilities, ...if opportunities for learning and personal growth do not exist or if they feel underutilized in their ...

  4. All Antibodies

    Type
    Collection
    ...Reactivity Recommended Applications PI Return to Top Do you have suggestions for other antibodies that should...

  5. Plasmid Collections

    Type
    Collection
    ...Rett Syndrome Plasmid Collection and Resource Center Do you have suggestions for other plasmids that should...

  6. Optogenetics Plasmid Collection

    Type
    Collection
    ...Vector Type Promoter PI Publication Return to top Do you have suggestions for other plasmids that should...

  8. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...PI Publication Last reviewed on: January 30, 2025 Do you have suggestions for other plasmids that should...

  12. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    ...customer place an Addgene order using a PO number? Why do I have to order directly from your website? Many ...sent to the contact email addresses provided. How do I place an order using a PO number? Placing an order... PO, learn more about how to create a quote. How do I pay the invoice? After your Addgene order ships...this article, check out our Help Center article How do I pay by purchase order (PO) number? You can find...

  13. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    ... news and updates. Scientists must do science to be happy. What do we have to change to ensure that all...all scientists can have successful careers doing science in some form? There is a lot of talk about the... behind and some just don't have the resources to do enough. There are still university research departments...

  14. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...researchers in the field. Dos and don’ts when publishing a scientific manuscript Dos: Make sure your paper...editor or reviewer over their decision. If you do object, do so in a respectful and professional manner. ...need to include in your manuscript. What information do I need to include in my paper? More and more journals...

  15. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...you in building a grant career. Do you have a passion for the arts? Do you find satisfaction in helping...Chat, and then our grant training and mentoring. How do I become a grant professional? Employment opportunities...healthcare facilities and with private businesses. How do you get the expertise needed to enter the grants ...helping your local food shelf raise money? Do you have experience with research grants? Are you interested...find work and target your professional development. Do you want to work in a particular area, e.g. education... childhood development, or community development? Do you want to focus on a part of the grant development...

  16. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...Wood of Duke University Medical Center. “While we do not yet have a comprehensive understanding of how... become resistant, an emerging theme is that they do so by activating signaling pathways controlling properties...contexts, so he assembled a plasmid kit designed to do exactly that. As described in the journal Science... world who are interested to use our libraries to do things - sometimes drug screens and sometimes totally...

  17. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    ...are trying to achieve: Do you want to permanently knock out or knock-in a gene? Do you want to activate ...Are you trying to create a single point mutation? Do you want to create a fusion with a reporter protein...appropriate expression system and delivery method: Do you need stable integration or is transient expression...sufficient? Which cell types will you be editing? Do you want to deliver the components as DNA, or would...surprised at the number of options available - how do you choose between them? One of the first steps is...

  18. Chemogenetics Plasmids

    Type
    Collection
    ...Description Gene/Insert Vector Type Promoter Tags PI Do you have suggestions for other plasmids that should...

  19. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ... what position do you want your gene to be in relation to the rest of the genes? And do you want it to...the Modular Cloning (MoClo) system and what it can do for you, and you’ve chosen one of the MoClo kits ...coding sequence, a terminator, or other plasmid part? Do you want to fuse your protein to any tags or fluorescent...your MoClo assemblies in silico before you start doing your cloning. Most sequence analysis software includes...preventing your overhangs from aligning properly. Do this sequentially for your Level 0, Level 1, and ...viability. When handling your MoClo plate, always do so in cold conditions and preferably on dry ice. ...

  20. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...have any questions, comments or suggestions, please do not hesitate to email me at [email protected] need a Nature/Cell/Science paper. You do need multiple first/corresponding author publications...exciting idea in a swamp of unnecessary details. You DO NOT need to present a detailed review of your field...field or the finer points of your techniques. You DO need to be prepared to know the literature of your...proteins is essential for human health. Failure to do so is implicated in several human diseases. Proper...bring in grants. What kind of colleague will you be? Do you understand what it takes to be successful in ...a Department's/Institution's success is the task. Do your homework. Learn what your future potential colleagues...
Showing: 101 - 120 of 771 results