We narrowed to 438 results for: ERG
-
TypeBlog Post...molecules of an RNA species with high specificity (Gootenberg et al., 2017). This system, dubbed SHERLOCK (...Find plasmids from this paper at Addgene. 3. Gootenberg, Jonathan S., et al. "Nucleic acid detection ...plasmids from this paper at Addgene. 6. Shmakov, Sergey, et al. "Discovery and functional characterization...
-
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog Post...sensitivity and detection - since they frequently undergo conformational changes after target binding, aptamers... biosensors. Aptabeacons Fluorescence resonance energy transfer (FRET) technology has been studied and... L. “Aptamer based electrochemical sensors for emerging environmental pollutants.” Front Chem. 2014; 2... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog Post...fashion while allowing for creative solutions to emerge. They enjoy the fact that each new challenge will...amount of time can sometimes become cauldrons on the verge of boiling. On other occasions, clients can be excessively...thoughtful planning and engagement, leaving mental energy to enjoy the more rewarding and educational parts... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post...approach, Zhang et al. turned to the CRISPR/Cas9 Synergistic Activation Mediators (SAM) system available from...Young WB, Khalili K, Hu W. CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent...Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA, Liang C. CRISPR/Cas9-Derived Mutations Both... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog Post...experiments (Rolland et al., 2016; Su et al., 2004; Taschenberger et al., 2017). Figure 3: Comparison...C. M., Cheng, F., Qiu, J., Carette, J. E., & Vandenberghe, L. H. (2018). An Alternate Route for Adeno-.... https://doi.org/10.1007/s11064-004-6881-1 Taschenberger, G., Tereshchenko, J., & Kügler, S. (2017). ... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog Post...there is a lack of expert mentorship during undergraduate and graduate studies. Students also need more... a rapidly changing, globalized environment re-emerging from international financial struggle, our new...mentorship in the field of Biomedicine to 78 undergraduate/graduate students from 5 independent universities... -
Plasmids 101: Using Transposons in the Lab
TypeBlog Post...delivery methods, but since TEs insert mostly in intergenic regions, gene disruption is less of a concern...to a single location in bacterial genomes, the Sternberg lab developed the INTEGRATE (Insertion of Transposable...transposons-the-jumping-genes-518/ Sandoval-Villegas N, Nurieva W, Amberger M, Ivics Z (2021) Contemporary Transposon Tools... -
RNA Interference in Plant Biology: New Tools for an Old Favorite
TypeBlog Post...both methods did a more comprehensive gene list emerge that accurately reflected the scope of genes known... straightforward positive selection of plants undergoing RNAi, and LIIbeta F 1-2 RNAi, which allows assembly...microscopic plants must be visually identified as undergoing RNAi through the fluorescent reporter and manually... -
Important Considerations When Using AAVs
TypeBlog Post...has been demonstrated that certain serotypes can undergo axoplasmic transport. These AAVs may move toward...Examining serotypes 1, 8 and 9, not only do they undergo retrograde and anterograde transport, but they ...avoid this rate limiting step and can immediately undergo replication and transcription. However, with scAAV... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post...substrate, luciferin, which releases energy as a photon. The photon energy is transferred to the acceptor fluorophore...pcDNA3.1+-pHlash, that use Bioluminescence Resonance Energy Transfer (BRET) were developed by the Johnson Lab...fusion protein (referred to as dCas9-VPR) had a synergistic effect on target gene activation. The novel dCas9...rely on the principle of bioluminescence resonance energy transfer (BRET), whereby photons emitted by a Renilla... -
Antibodies 101: Flow Cytometry
TypeBlog Post...permeabilization with detergents such as triton-x, saponin, or tween. Take care when using strong detergents like triton-x...resources References: Bonner WA, Hulett HR, Sweet RG, Herzenberg LA (1972) Fluorescence Activated Cell Sorting... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog Post.... Two low-energy photons (typically from the same laser) cooperate to cause a higher-energy electronic...photoconvertible Keade proteins (protein that undergoes light-induced irreversible photoconversion from... -
Tags and Other Markers
TypeCollection...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control... -
Neuroscience
TypeCollection...ready-to-use recombinant monoclonal antibodies undergo application-specific validation and quality control... -
TREAT-AD Plasmid Collection
TypeCollection...disease (AD) by generating validated resources for emerging targets in AD and creating high-quality, well-... -
Antibodies
TypeCollection... are expressed from plasmids. These antibodies undergo application-specific validation and consistent ... -
KLF Research Plasmids
TypeCollection...Michel Sadelain James Thomson Tim Townes Robert Weinberg Knut Woltjen Shinya Yamanaka Feng Zhang About ... -
Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection
TypeCollection... Allen WE, Singh A, Kuksenko O, Abudayyeh OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, ... -
All Antibodies
TypeCollection...recombinant antibodies. These monoclonal antibodies undergo application-specific validation and quality control... -
CRISPR Plasmids - C. elegans
TypeCollection...In vitro transcription none, need Cas9 plasmid Sternberg 177783 L4440_BioBrick-sgRNA T7 BbsI, BsaI Ingestion...