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We narrowed to 430 results for: ERG

Showing: 101 - 120 of 430 results

  2. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...obstacles when working with virus: 1. Viral vectors undergo DNA rearrangements. Depending on the composition...repeat sequences present in the LTR, the vector may undergo genomic rearrangements during replication. To avoid...immunotherapy." Acute Leukemias VIII. Springer Berlin Heidelberg, 2001. 291-306. PubMed PMID: 10634178. 8. Murnane...

  3. Zebrafish Plasmid Collection

    Type
    Collection
    ...ortholog in zebrafish, this species has more recently emerged as a model system for human disease and translational...

  4. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ... media, while others remain cell-associated (Vandenberghe et al., 2010). For certain in vitro and in vivo...rAAV sample has to be digested with DNase before undergoing titering, to remove residual plasmid DNA carried...Article 10. https://doi.org/10.1089/hum.2009.223 Vandenberghe, L. H., Xiao, R., Lock, M., Lin, J., Korn, M...

  6. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...I knew that when I was “managing” a couple of undergrads back in grad school. Both were hard-working scientists-in-training...reviewing the results of my Myers-Briggs test:  my undergrads were different people! More specifically, they...time I could likely have retained this talented undergrad. The point here is managing is not “one size ...

  7. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...surface of the protein where the nascent polypeptide emerges from the exit channel.   He then said “I hadn’t...accurately suggests the potential of this protein to undergo conformational shifts. This backbone model of Cas9...conformational gymnastics that this protein must undergo as it interrogates your genome. If you are interested...

  8. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    ...takes roughly 10 days for an egg to develop and emerge as an adult fly at 25°C. At low temperatures like...the day and throughout daytime. They mostly stop emerging at night. An incubator with a light cycle makes...makes for very predictable work! When flies first emerge, their bodies will be very light in coloration ...

  9. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...their viruses; food allergy labs use standard curves with ELISAs to measure allergen concentrations in ...What do a viral vector production facility, food allergy testing lab, and the grad student down the hall...

  10. An Introduction to Adenovirus

    Type
    Blog Post
    ... zoonotic? A systematic review of the evidence. Emerg Microbes Infect, 8(1), 1679–1687. https://doi.org...of a Cytopathogenic Agent from Human Adenoids Undergoing Spontaneous Degeneration in Tissue Culture. Proc...M., Foresythe, A., Huh, H. J., Bazinet, A. L., Bergman, N. H., Bull, R. L., Garcia, K. Y., Goodrich, J...

  11. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...Pigmentosa. I quickly became fascinated by this emerging technology and its potential for the treatment...cost of clinical development. As a result, Luk Vandenberghe and colleagues at MEEI co-founded a non-profit...approves gene therapy for blindness 2. Gene Therapy emerges from disgrace to be the next big thing, again 3...

  12. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...third chromosome, ten base pairs in length. An emerging family of sequencing techniques function as a ...coordinates into meaningful experiments. Table 1: An emerging family of "GPS for the genome" techniques Technique...going. Appendix DNAse Hypersensitive Sites and Intergenic CRISPRs Yours truly generated the DHS data set...

  13. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...the earliest uses of GFP. Fluorescence resonance energy transfer (FRET): This is used to study the interactions...proteins or between two domains of a protein that undergoes conformational change. Typically, two fluorescent...come into close proximity, the two halves of EGFP undergo folding, maturation, and fluorescence. Biosensors...

  14. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...sensitivity and detection - since they frequently undergo conformational changes after target binding, aptamers... biosensors. Aptabeacons Fluorescence resonance energy transfer (FRET) technology has been studied and... L. “Aptamer based electrochemical sensors for emerging environmental pollutants.” Front Chem. 2014; 2...

  15. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...fashion while allowing for creative solutions to emerge. They enjoy the fact that each new challenge will...amount of time can sometimes become cauldrons on the verge of boiling. On other occasions, clients can be excessively...thoughtful planning and engagement, leaving mental energy to enjoy the more rewarding and educational parts...

  16. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ... was designed in response to requirements that emerged from intensive engagement with a wide range of ...research computing. The two guiding principles emerging from the engagement with the Wisconsin research...fundamental driver of these principles and has emerged as a byproduct of connectivity. Between 2012 and...

  17. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...molecules of an RNA species with high specificity (Gootenberg et al., 2017). This system, dubbed SHERLOCK (...Find plasmids from this paper at Addgene.  3. Gootenberg, Jonathan S., et al. "Nucleic acid detection ...plasmids from this paper at Addgene. 6. Shmakov, Sergey, et al. "Discovery and functional characterization...

  18. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...approach, Zhang et al. turned to the CRISPR/Cas9 Synergistic Activation Mediators (SAM) system available from...Young WB, Khalili K, Hu W. CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent...Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA, Liang C. CRISPR/Cas9-Derived Mutations Both...

  19. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...experiments (Rolland et al., 2016; Su et al., 2004; Taschenberger et al., 2017).     Figure 3: Comparison...C. M., Cheng, F., Qiu, J., Carette, J. E., & Vandenberghe, L. H. (2018). An Alternate Route for Adeno-.... https://doi.org/10.1007/s11064-004-6881-1 Taschenberger, G., Tereshchenko, J., & Kügler, S. (2017). ...

  20. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...there is a lack of expert mentorship during undergraduate and graduate studies. Students also need more... a rapidly changing, globalized environment re-emerging from international financial struggle, our new...mentorship in the field of Biomedicine to 78 undergraduate/graduate students from 5 independent universities...
Showing: 101 - 120 of 430 results