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...AAV1 AAV2 AAV5 AAV8 AAV9 AAVrg PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity Serotype...We provide high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV... See our Optogenetics Plasmids and Optogenetics Guide page for more information about this field. Use ... found to match all requested criteria. Please consider using fewer filtering options. Browse all Optogenetics...

...AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Promoter Sensor Label Activity Serotype PI Acetylcholine... found to match all requested criteria. Please consider using fewer filtering options. *AAVrg = retrograde...

... R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target Target Species Host Species Isotype...produce scFvs from transfected mammalian cells. Addgene ID Plasmid Hybridoma Target Target Species Host Species...nanobodies from transfected bacterial cells. Addgene ID Plasmid Hybridoma Target Target Species Host Species...IgG2a 188173 Anti-NPY2R/Neuropeptide Y receptor type 2 [L119/134R] NPY2R/Neuropeptide Y receptor type 2 Human...Parvalbumin Rat Mouse IgG2b 206639 Anti-Neuropeptide Y [L115/13R-2b] Neuropeptide Y Human Mouse IgG2b 206640 Anti-VGAT... 211594 Anti-NPY2R/Neuropeptide Y receptor type 2 [L119/106R-2b] NPY2R/Neuropeptide Y receptor type 2 ...Rat Mouse 190505 NPY/Neuropeptide Y scFv [L115/13] L115/13 scFv 2t NPY/Neuropeptide Y Human Mouse 190506...

...CCN3, IGFBP9 NPFF neuropeptide FF-amide peptide precursor FMRFAL NPPA natriuretic peptide precursor A ANF... PIK3CD phosphoinositide-3-kinase, catalytic, delta polypeptide p110D PIK3CG phosphoinositide-3-kinase...natriuretic peptide precursor B BNP NPPC natriuretic peptide precursor C CNP NPR1 natriuretic peptide receptor... HBGF8, HBNF, NEGF1 PYY peptide YY PYY1 QRFP pyroglutamylated RFamide peptide 26RFa, MGC119794, P518 RABEP1...class I polypeptide-related sequence A FLJ60820, MGC111087, PERB11.1 MICB MHC class I polypeptide-related...

... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment...more information on the wide range of CRISPR technologies, visit our CRISPR Guide . There are many different...components (Cas enzyme and guide RNA) for your experiment (Figure 1). You will decide how to express Cas9, ...both a guide and a template. In general, edits must be downstream (3’) of the nick site, and guides that...increase specificity, consider using an engineered Cas enzyme with higher fidelity. In addition to off-...screens in this guide, lentiviral vectors are a common choice for conducting genome-wide screens using ...experiments for different organisms. This page provides a general framework to get you started using CRISPR...

...along with the guide RNA. For most applications, we recommend using Cas9 with the single guide RNA (sgRNA)...that adding an additional guanine nucleotide to the 5’ of 20-bp guide can increase targeting efficiency...PX330; SpCas9 and single guide RNA 48138 : PX458; SpCas9-2A-EGFP and single guide RNA 62988 : PX459; SpCas9...2A-Puro and single guide RNA 48873 : PX460; SpCas9n (D10A nickase) and single guide RNA 48140 : PX461;...nickase) and single guide RNA 62987 : PX462; SpCas9n-2A-Puro (D10A nickase) and single guide RNA 48137 : PX165...below. Pooled genome-wide human SAM library SAM can be combined with a human genome-wide library to activate...that adding an additional guanine nucleotide to the 5’ of 21-bp guide can increase targeting efficiency...

...Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide to using CRISPR plasmids in your lab, from...Model-based Analysis of Genome-wide CRISPR/Cas9 Knockout (MAGeCK). This tool can identify positively or negatively...location, pasted nucleotide sequence, or a sequence text file you upload. Currently supports a wide selection...screens targeting a long or short list of genes provided by the user. Developed by the Fredrik Wermeling... 2.0 (Link opens in a new window) A tool that identifies putative target sites for S. pyogenes Cas9, S...Institute. Cas-OFFinder (Link opens in a new window) Identifies gRNA target sequences from an input sequence ...online predictor (Link opens in a new window) Identifies candidate sgRNA target sites by off-target quality...

...display less off-target activity. High fidelity Cas9s: Three high fidelity Cas9 variants , SpCas9-HF, eSpCas9...Joung JK. 2016. High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects. ... Genome Engineering You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts...crRNAs). Through sequence homology, these crRNAs guide a Cas nuclease to the specified exogenous genetic...but it is not invincible. Bondy-Denomy et al. identified the first set of anti-CRISPR genes in bacteriophages... screens. Pooled gRNA libraries can be used to identify genes that are important to a given phenotype....sequencing, CRISPR is a robust system for genome-wide screening. CRISPR Technological Advances CRISPR ...

...Ral guanine nucleotide dissociation stimulator RAPGEF RAPGEF1 RAPGEF2 Rap guanine nucleotide exchange factor...GTPase and is a member of the G protein (guanine nucleotide binding protein) family. GTPases serve as molecular...isoforms, individual links to each gene page are provided below. Color is used for clarity and does not ...isoforms, individual links to each gene page are provided below. Symbol Name AKT AKT1 AKT2 AKT3 v-akt murine...activating protein 35 ARHGEF2 Rho/Rac guanine nucleotide exchange factor 2 CCND CCND1 CCND2 CCND3 Cyclin...factor ECT2 Epithelial cell transforming 2 EGFR Epidermal growth factor receptor EIF4EBP EIF4EBP1 EIF4EBP2...2 like 2 NFKB1 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 PAK PAK1 PAK2 PAK3...

...CRISPR guides. The first one binds to sfgfp with either 0, 10, 11, 14, or 20 matching nucleotides, the ...Repression Plasmids You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts...decreasing the expression level. Mismatches between the guide RNA and the target sequence lower the repression...mcherry with either 0, 11, 18, or 20 matching nucleotides. Together, these plasmids allow to explore the...controlled using a plasmid‐borne CRISPR array coding for guide RNAs (diamonds) interspaced with CRISPR repeat motifs...

...Looking for E. coli or yeast plasmids? Addgene has a wide variety of tools for work in these model organisms... our deposited tools and resources, or read our guide on assembly standards. Browse Plasmids by Microbe...community that aren't in the repository? Help us widen our resources for the community by depositing your...Microbiology General CRISPR Resources Read our CRISPR Guide for a general introduction to CRISPR technology....experiment. Synthetic Biology Assembly Standards Guide Bacterial Expression Systems : Find plasmids to ...bacterial expression system. Fluorescent Protein Guide : Addgene's extensive fluorescent protein plasmid...

...backbone to use, you have many elements to consider. Here is a guide to a selection of Addgene's empty vector...such as Flag or HA, will allow you to easily identify your protein using an antibody against that epitope... our full Fluorescent Protein (FP) Guide and the Michael Davidson FP Collection NLS Nuclear...N-terminal His and NusA fusions Return to top Function Besides functions such as protein purification mentioned... plasmid in Pseudomonas species Vectors for RNA-guided genome editing in plants Visit our CRISPR Plasmids...Protein-protein interaction Proximity-dependent biotin identification (BioID) MCS-BioID2-HA or myc-BioID2-MCS - To...

...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...you will need to design and clone in a gRNA(s) to guide the Cas9 protein to your target sequence, as well...plasmid. The Mendenhall and Myers labs have also provided their protocol for homology arm cloning: Mendenhall...single plasmid targeting vector that encodes both a guide RNA transcript and a repair template for an individual...epitope tags. The plasmids in the following articles provide PCR templates for amplification of the tag (eg ...Nucleic Acids Res. 2014 The Förstemann lab has provided detailed protocols for N- or C-terminal tagging...

...control methods. Learn More Guide New to using antibodies? In our Antibody Guide, we define key terms, summarize...experts in the field to develop new antibodies and provide experimental data for recommended applications....Interested in our production process? At Addgene, we provide transparency in our production process ...techniques, and explain common applications. View Guide Need Some Help? Browse some of our other resources...

...phosphate-buffered saline containing 1 mM sodium azide. This buffer is not compatible for use in live cells...sample is separated via a denaturing SDS-PAGE gel alongside serial dilutions of a standard antibody of known... undergoes testing, either in-house or through outside labs, to confirm recognition of the expected target...parental plasmid sequence to confirm the antibody’s identity. For more details on protocols see the Addgene...at room temperature. However, Addgene determines ideal shipping conditions for each order based on the ...

...to be associated with the disease and implicate a wide range of cellular processes. The vast majority of...affects movement. More than ten million people worldwide are living with PD, mostly people over the age...associated with Alzheimer's disease, through large genome-wide association studies (GWAS) or other large scale ...modification and target species. You can also find our guide to using plasmid pooled libraries . Use the ultracompact...animal models, purified protein, and more. Plasmids provided by the foundation can be found on our Michael ...

...activator peptide activates gene expression. Interfere dCas9 alone, or fused to a repressor peptide, inhibits...parasites, and Xenopus. Addgene CRISPR Resources CRISPR Guide : Essential background information on CRISPR and... CRISPR libraries are a powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag ...

...subunits, individual links to each gene page are provided below. Color is used for clarity and does not ...subunits, individual links to each gene page are provided below. Symbol Name 14-3-3-σ Stratifin Apaf-1 Apoptotic...apoptosis inducing protein 1 p53R2 p53 inducible, ribonucleotide reductase M2 B (RRM2B) PAG608 Zinc finger, ... PIDD p53-induced death domain protein 1 PIGs Etoposide induced 2.4 PIRH-2 Ring finger and CHY zinc finger...

...Sequencing results are analyzed to confirm the identity and integrity of the viral genome and the absence...Samples of viral preparations are subjected to polyacrylamide gel electrophoresis (PAGE) followed by silver...negative staining. Empty vector particles can be identified after negative staining and appear darker than...to the cleared viral preparation pool. Proviral Identity Confirmation and Sterility All of our lentiviral...

...the time of shipment. If any discrepancies are identified during this analysis, you will be notified by... Addgene performs QC measures which verify the identity of the viral vector, but not the function. Upon... years of the order being shipped, Addgene can provide the QC validation data performed on the material...