Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 876 results for: NSI;

Showing: 101 - 120 of 876 results

  1. AAVED

    Type
    Collection
    ...lab (main issues/factors that researchers should consider when planning experiments). What resources might...

  2. p53 Pathway

    Type
    Collection
    ...E3 ubiquitin protein ligase PTEN Phosphatase and tensin homolog PUMA BCL2 binding component 3 Reprimo TP53...

  3. Zebrafish Plasmid Collection

    Type
    Collection
    ...Neurodegenerative Disorders - Nicholas Cole Lab. A kit consisting of plasmids with four promoters, six fluorophores...

  4. Control AAV Preps

    Type
    Collection
    ... found to match all requested criteria. Please consider using fewer filtering options. Browse all Control...

  5. Ras Pathway

    Type
    Collection
    ...non-catalytic subunit gamma PTEN Phosphatase and tensin homolog RAC RAC1 RAC2 RAC3 Ras-related C3 botulinum...

  7. CRISPR References and Information

    Type
    Collection
    ...entries describing enzyme activity, experimental considerations, and more. CasPEDIA is a community resource...

  8. Optogenetics AAV Preps

    Type
    Collection
    ... found to match all requested criteria. Please consider using fewer filtering options. Browse all Optogenetics...

  9. Retrograde AAV viral preps

    Type
    Collection
    ... found to match all requested criteria. Please consider using fewer filtering options. Browse all Retrograde...

  10. Caltech Systemic Capsids

    Type
    Collection
    ...do you want to target CNS or PNS? Then consider species, and then cell type. For CNS: For infant...

  11. Biosensor AAV Preps

    Type
    Collection
    ... found to match all requested criteria. Please consider using fewer filtering options. *AAVrg = retrograde...

  12. Validated gRNA Sequences

    Type
    Collection
    ... Keep in mind that additional factors must be considered before using a validated gRNA sequence in your...

  14. Cre-lox system

    Type
    Collection
    ...Nestler 68552 HRE:GFP-pGK:Cre Cre and hypoxia-responsive GFP reporter HRE Lentiviral Jacks 69570 pAAV-...

  15. Antibody Guide

    Type
    Guide
    ...Figure 1. The two arms of the Y structure are responsible for binding the antigen, and are called the antigen...isotype of the antibody. B cells, the immune cell responsible for producing antibodies, can change the constant...binding site. In B cells, the genetic region responsible for the variable region goes through a randomized...valency of 2 IgE Protects against parasites and is responsible for driving allergic reactions such as anaphylactic...requirements, monoclonal antibodies are typically more expensive than polyclonal antibodies. Environmental factors... Minibodies are engineered antibody fragments consisting of two scFvs bound to a CH3 domain. Fab fragments... protein. Fluorophores Fluorophores are light-sensitive molecules which emit a specific wavelength of ...

  16. Plan Your Experiment

    Type
    Guide
    ...apply to using CRISPR in other organisms. First, consider the genetic manipulation that is necessary to ...Manipulation Application Cas9 gRNA Additional Considerations Knockout Permanently disrupt gene function ...ribonucleoprotein (RNP) complexes are advantageous due to the transient Cas9 expression. The table below summarizes the...details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or gRNA in a ...limit (only compatible with smaller Cas enzymes) Transient or stable expression of SaCas9 and/or gRNA Infects...cells through microinjection or electroporation Transient expression of CRISPR components Expression decreases...which is delivered to cells using cationic lipids Transient expression of CRISPR components Short window of...

  17. Optogenetics Guide

    Type
    Guide
    ... the following enhancements: Increased light sensitivity Example: ArchT Increased photocurrent amplitude... Volvox carteri 570 Chronos High-speed, light-sensitive channel from Stigeoclonium helveticum 500 Chrimson...from Halorubrum strain TP009. Improved light sensitivity over Arch. 566 eArchT 3.0 ArchT with addition... delivery system. Here are some key factors to consider: Optogenetic excitation or optogenetic inhibition...neural populations at distinct times. Temporal considerations. Temporal precision is key in optogenetic experiments...Rhodopseudomonas palustris bacteria. Key feature is the sensitivity to 740- to 780-nm near-infrared light. 740–780...488 LOV domains LOV-based Light-oxygen-voltage-sensing (LOV) domains are protein sensors used by many ...

  18. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...the viral genetic material inside. The AAV genome is ~4.7 kb long and consists of two open reading frames...ITRs is limited by the physical space available inside of the small AAV capsid and directly correlates...Genome Integration Recombinant AAVs are generally considered to not integrate into the host genome. When wild-type...during production, as it carries the Cap region responsible for the production of capsid proteins. AAV2 is...it was the first to be well-characterized and extensively studied, and has a broad tissue tropism. Table...information. Self-complementary AAV (scAAV) One downside of the use of AAVs as viral vectors is their single-stranded...common points of entry. Biosafety should always be considered with respect to the precise nature of experiments...

  19. Chemogenetics Guide

    Type
    Guide
    ...molecule ligand. The ideal chemogenetic tools are unresponsive to native ligands and are engineered to respond... development of mutated receptors that while unresponsive to endogenous ligands, could be activated by... coupled receptors, but unlike RASSLs, show insensitivity to endogenous ligands, have low constitutive...proteins of a light-emitting luciferase and a light-sensing optogenetic element. When the luciferase substrate...luciferase-fluorescent protein fusions, to more-sensitive opsins has led to improved LMOs with increased...chemogenetics experiment, here are some things to consider: Chemogenetic activation or inhibition. Do you... a study in rats using an hM4Di receptor might consider an alternative DREADD ligand such as DCZ versus...

  20. CRISPR Guide

    Type
    Guide
    ...fusing the anti-CRISPR protein AcrIIA4 with the photosensitive LOV2 domain . Several labs have also controlled...There are two main types of base edits: Base transitions - edit to same classification of nucleotide base...pyrimidine-to-pyrimidine), which catalyze base transitions. Cytosine base editors are created by fusing ...integrated along with the sequence, so the edit is not considered “scarless”. Figure 7: Overview of CRISPR transposases... target gene function. It has also been used extensively to screen for novel genes that regulate known...simplest dCas9-based activators and repressors consist of dCas9 fused directly to a single transcriptional...silencing across much of the human genome following transient expression of the dCas9 fusion, with the epigenetic...
Showing: 101 - 120 of 876 results