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We narrowed to 952 results for: OMP;

Showing: 101 - 120 of 952 results

  2. Open Enzyme Collection

    Type
    Collection
    ...opens in a new window) . Open Enzymes are also compatible with the E. coli Protein Expression Toolkit (...

  3. Viral Production

    Type
    Collection
    ...Purity Purity of AAV preparations is assayed by comparing the relative stoichiometric ratios of the viral...

  4. Control AAV Preps

    Type
    Collection
    ...under general promoters. These AAV can be used to compare the activity of different serotypes. Serotype AAV1...

  8. CRISPR References and Information

    Type
    Collection
    ...CRISPResso CRISPResso2 is a robust and easy-to-use computational pipeline for the analysis of genome editing ...

  9. Deisseroth INTRSECT Collection

    Type
    Collection
    ...sub-populations has generally been limited to single-component cellular definitions (e.g. neurons defined by ...

  10. Plasmids for Stem Cell Research

    Type
    Collection
    ...and Xeno-Free Culture Conditions: A Clinically Compliant Approach. Stem Cells Transl Med. 2015 Mar 5. pii...

  11. Genetic Code Expansion

    Type
    Collection
    ...can bind to the mRNA (hence, the anticodon is complementary to the codon), the amino acid from the tRNA ...

  12. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ... PCR products are fused to a joining fragment comprising a κ light chain constant domain (C L ) and the...

  13. Protocol - Bacterial Transformation

    Type
    Protocol
    ...are commonly referred to as 'competent cells.' Many companies sell competent cells, which come frozen and...instructions that came with your competent cells. Pro-Tips Commercial competent cells range significantly in... LB or SOC media Competent cells DNA you'd like to transform Procedure Take competent cells out of -80...efficiency is needed follow the complete protocol. Thaw the competent cells in your hand instead of on...positive control (many companies include a positive control plasmid with their competent cells) to ensure that... To save money, many labs also make their own competent cells. This is a relatively simple procedure and...DNA (usually 10 pg - 100 ng) into 20-50 μL of competent cells in a microcentrifuge or falcon tube. GENTLY...

  14. CRISPR Guide

    Type
    Guide
    ...type I Cas effector, composed of several separate Cas enzymes that form a complex called Cascade (see ...transposase components. Type I (generally) includes tnsA , tnsB , tnsC , and tniQ . In comparison, type V-...Cascade ( C RISPR- as sociated c omplex for a ntiviral de fense) complex to initiate DNA degradation. Cascade...Cascade C RISPR- as sociated c omplex for a ntiviral de fense; a complex of multiple Cas enzymes including...nuclease pair for every genomic target. Due to its comparative simplicity and adaptability, CRISPR rapidly became...approach. Engineered CRISPR systems contain two components: a guide RNA ( gRNA or sgRNA ) and a CRISPR-associated...Cas enzyme). The gRNA is a short synthetic RNA composed of a scaffold sequence necessary for Cas-binding...

  15. Antibody Guide

    Type
    Guide
    ...capture protein:DNA complexes. After capture, the DNA is released from the complex and can be sequenced...is licensed to multiple companies for sale. Before purchasing, directly compare the product information... size comparison of peptides. Independent antibody validation This method validates by comparing your ...configuration of a protein or a protein:protein complex. Due to their increased manufacturing requirements...from antibody fragments (shown in Figure 2) to completely unrelated molecules. Examples include: scFv -...nanostructures. Figure 2: An antibody (IgG, far left) compared to alternative affinity reagents (left to right...conjugating and signaling molecules, as some buffer components can interfere with the conjugate or conjugation...

  16. Modular Cloning Guide

    Type
    Guide
    ...genomics, and more complex metabolic engineering and synthetic biology, compatible with PhytoBrick parts...from different combinations of a common set of components (Weber et al., 2011). This method, based on Golden...restriction cut site, multiple modules with complementary overhangs can be digested and ligated in a single...standardized modules. In the first assembly step, compatible Level 0 parts are directionally assembled into...Level 2 vectors permits the creation of even more complex constructs constrained only by the ability of E...transformation. Figure 1: Overview of the MoClo Toolkit components and assembly. Image from Weber et al., 2011. ...(Lohmann) with additional Level 0 modules and components to create plasmids with multiple transcriptional...

  17. Lentiviral Vector Guide

    Type
    Guide
    ...laboratory, the non-required components have been removed, and the required components have been separated into...our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and common...hence the use of “retro”. Lentiviruses have more complex genomes, containing packaging genes and accessory...require different isoforms of these packaging components. Therefore, lentivirus may not be efficiently...) necessary for integration. Not all of these components are necessary for lentiviral production in the...safety. For a summary of all lentiviral plasmid components, see the Lentiviral Plasmid Elements table . ...improvements and the potential for replication-competent lentivirus production — that is, creation of viral...

  18. CRISPR Library Amplification

    Type
    Protocol
    ... Thaw 4 tubes of electrocompetent cells on ice for 15-20 minutes or until completely thawed. Chill a box...obtained. Ensure that electrocompetent cells are being used. Chemically competent cells will not provide... to the inherent possibility of altering the composition of the library. Bottlenecks, fitness differences... in the morning to ensure that outgrowth and competition are limited. Equipment Table top centrifuge BioRad...MicroPulser TM , Bio-Rad 1652100) Reagents 200 µL electrocompetent cells (Default: 4 tubes of Endura Duos, Lucigen...Stbl4 cells or other ultra-high efficiency electrocompetent cells that are suitable for unstable or recombination-prone...recombination-prone DNA. The use of electrocompetent cells is essential to ensure high efficiency uptake...

  19. Molecular Cloning Techniques

    Type
    Guide
    ...overhang (blunt ends). Two pieces of DNA that have complementary overhangs, or which are both blunt-ended, can...This can make it difficult at times to identify compatible restriction enzymes that cut your insert or backbone... in the DNA sequence and can be time-consuming compared to other cloning methods. Read more in our Restriction... into a Gateway donor plasmid, which contains compatible attP sites via a proprietary BP clonase, creating...Addgene), it can be rapidly shuttled into any compatible Gateway destination vector, which contains attR...restriction cloning, once your project has been completed via Gateway cloning. Addgene's collection contains...method that relies on the hybridization of the complementary base pairs adenine (A) and thymine (T). TOPO...

  20. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...formation of replication-competent viruses. While AAV themselves are replication incompetent, there is a rare ... viral vector guide to learn about AAV vector components, production and common uses. Science...bilayer surrounding the viral particle and are only composed of a protein capsid and the viral genetic material...simplex virus to replicate in the host cell and complete the lytic cycle. AAVs are therefore non-pathogenic...packaging plasmid. For a summary of all AAV plasmid components, see the AAV Plasmid Elements table . Figure ...have limited restriction sites or may only be compatible with certain cloning methods (such as a Gateway... so be sure to confirm your chosen plasmid is compatible with cloning methods available in your lab. When...
Showing: 101 - 120 of 952 results