...genomics, and more complex metabolic engineering and synthetic biology, compatible with PhytoBrick parts...from different combinations of a common set of components (Weber et al., 2011). This method, based on Golden...restriction cut site, multiple modules with complementary overhangs can be digested and ligated in a single...standardized modules. In the first assembly step, compatible Level 0 parts are directionally assembled into...Level 2 vectors permits the creation of even more complex constructs constrained only by the ability of E...transformation. Figure 1: Overview of the MoClo Toolkit components and assembly. Image from Weber et al., 2011. ...(Lohmann) with additional Level 0 modules and components to create plasmids with multiple transcriptional...