...use in plants. These resources include tools for research using plant systems such as Arabidopsis, wheat...MoClo Plant Parts II and Infrastructure Kit for hierarchical assembly of plant multigene constructs - Johannes...gene of interest for expression in plants. You can search the table for features you are looking for, such...luminescent proteins for expression in plants. You can search the table for features you are looking for, such...molecular biology data for Arabidopsis thaliana research. BAR The Bio-Analytic Resource for Plant Biology...expression in the grasses. OpenPlant Synthetic Biology Research Centre (Link opens in a new window) - The initiative...

...used for research, it is the lentiviral genome that encodes genetic material that the researcher wants delivered...most common and useful types of viruses used in research. Lentivirus can transduce both dividing and non-dividing...available at Addgene have been designed for increased researcher safety. Read our Lentiviral Guide for more information...Plasmids One safety feature of lentivirus used in research is that the components necessary to produce an...Please see our CRISPR Empty gRNA plasmids table and search "lenti". ID Plasmid Generation Description PI 8453...

...Shared Research Tools Labs affiliated with MJFF have also generated a number of Shared Research Tools ...Foundation Plasmid Resource MJFF Plasmids Shared Research Tools Addgene is working with The Michael J. Fox...'s disease plasmid resource for the scientific research community. Michael J. Fox Foundation Plasmids ... SNCA and other proteins of interest. Browse or search the full list below. ID Plasmid Gene/Insert Mutations...

....2022.11.026 . TF ORF barcode PCR for NGS QC and bulk screening (85 KB) TF ORF barcode dial out PCR for 10X...transcription factor isoform has a unique 24 bp barcode that simplifies identification of the transcription... KR, Kirchgatterer PC, Verdine VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh OO... open reading frames. Each isoform is uniquely barcoded with a 24 bp sequence, to simplify identification...

...Disease Research Find deposited plasmids for specific disease research areas. Cancer Research Plasmids...Collection Neuroendocrine Tumor Research Foundation Neurodegeneration Research Collection Parkinson's Disease...

...intruders, antibodies are also extremely useful to researchers in a lab. When an antibody binds tightly to a...name a few. Traditional sources of antibodies for research purposes include animals or hybridoma cells, but...antibodies extensively validated for neuroscience research applications from the NeuroMab/Trimmer Lab Recombinant...expression of antibodies, nanobodies or ScFvs. Use the search bar or sort buttons to find antibody plasmids for...Nanobodies, Sybodies, or ScFVs Secondary antibodies (search for "anti-mouse" or "anti-rabbit") ID Plasmid Description...

...with fairly high specificity. Fifteen years of research has shown that when fused to a nuclease or activation...interview with Adam Bogdanove ), progress on TALe research has been swift. The incentives are high. Since...potential targetable sites approximately every 35bp. Researchers are still determining the importance of context...matter of days. In an open access Nucleic Acids Research article that came out in the spring of 2011, the...labs that continue to hone these tools and the researchers who are learning to wield them. Learn more about...

...gene of interest, please search our full plasmid collection using the search bar at the top of this page...pCS2plus-TDP-43 TARDBP CMV ALS Marco Morsch 162609 pCS2plus-TDP-43 M337V TARDBP CMV ALS Marco Morsch 162611 pCS2plus-mVenus1...CMV ALS Marco Morsch 162612 pCS2plus-mVenus1-155-GGGS-TDP43 M337V TARDBP mVenus CMV ALS Marco Morsch 162614... ALS Marco Morsch 162615 pCS2plus-mVenus1-155-I152L-GGGS-TDP43 M337V TARDBP mVenus CMV ALS Marco Morsch...Browse neurodegeneration plasmids for research related to Alzheimer's Disease, ALS, Frontotemporal Dementia...large collection of neurodegeneration plasmids for research related to Alzheimer's Disease (AD), Amyotrophic...John Gearhart 44333 pMX_mTBP TBP LTR Parkinson's Marc Timmers 44978 GST-FUS-WT FUS GST tac ALS Robin Reed...

...Protein Tagging Plasmids for Stem Cell Research Neurodegeneration Research Collection The iPSC Neurodegenerative...from this effort will be broadly shared with the research community. To this end, all plasmids that result...the project efforts will be made available for research purposes in the iNDI plasmid collection at Addgene...

...Other/Marchantia polymorpha MpU6 none S. pyogenes Hara-Nishimura pMpGE_En03 71535 Other/Marchantia polymorpha...To find the gRNA vector for your system, you can search or sort this table based on: Your particular expression...sgRNA 46170 Worm PCR (see paper) none S. pyogenes Calarco SP6-sgRNA-scaffold 47912 Worm AflII none S. pyogenes...U6 yes, cut S. pyogenes mCherry Savage pBLO1808_arC9_2xNLS_human 74491 Mammalian U6 yes, cut S. pyogenes...Bacteria J23119 none S. pyogenes Wang pBLO1811_arC9_noNLS_human 74494 Mammalian U6 yes, cut S. pyogenes...

...nickase plasmids fused to reverse transcriptase for search and replace gene editing....Design Tools CRISPR Blog Posts Prime editing is a “search and replace” gene editing method in which Moloney...the opposite strand is added . Browse, sort, or search the tables below for CRISPR prime editing plasmids...editing are highlighted in the table below. Use the search bar to find a gRNA vector based on expression system...

...replicate plasmids of all types, but beyond that, researchers also use bacteria like E. coli and their relatively...to help you harness bacterial genomes for your research. Plasmid ID Technique PI Purpose pCas9 42876 CRISPR...Purpose pRU Series Various Plasmids Cloned by researcher Fluorescent protein, GUS, and LacZ Derivatives... One-Hybrid Series Various Plasmids Cloned by researcher Growth of strain USO hisB- pyrF- rpoZ- on plates...Reagents Kit Various Plasmids Sequence chosen by researcher Beta-galactosidase Keith Joung Transcription ...

...website containing a module database where researchers can search for available modules, as well as find ...database with links to resources and information. Researchers can obtain the desired modules to perform their...sgRNA-PBS. Since Casilio modules are orthogonal, researchers can use any combinations of modules as is, allowing...

...alleles. To introduce specific genomic changes, researchers use ssDNA or dsDNA repair templates with homology...Epigenetics To make targeted epigenetic modifications, researchers have fused catalytically dead dCas9 to epigenetic... tables on Addgene's Empty gRNA Vectors page to search based on factors such as selectable marker or cloning...mammalian vectors, simply type "Mammalian" into the search bar. When using CRISPR, you will need to express...

... pAAV-nEF-Con/Fon-Arch3.3-p2a-EYFP Cre AND Flp 137149 pAAV-nEF-Con/Foff 2.0-Arch3.3-EYFP Cre AND NOT Flp... Flp FRT/F5 137150 pAAV-nEF-Coff/Fon-Arch3.3-p2a-EYFP Flp AND NOT Cre 137151 pAAV-nEF-NpHR3.3-EYFP None...Nucleus. Neuron 102(3):636-652. PMID:30905392 Marcinkiewcz CA, Mazzone CM, D'Agostino G, Halladay LR, Hardaway...

...recombinant antibodies to help facilitate scientific research. We are building a collection of affinity reagents...Addgene values transparency and accessibility in research and therefore makes the sequences of these affinity...of antibodies developed and validated for many research applications. Browse Antibodies Production Interested...

...Optogenetics Channelrhodopsins Halorhodopsins Archaerhodopsins Recombinases Cre Flp Dre Biosensors Calcium...Function" pages to the left or can be found using a Search of Viral Preps and filtering by the serotype needed...provide high-quality AAV vectors to the academic research community. The entire Penn Vector Core catalog...

...Addgene values transparency and accessibility in research. For each antibody, we provide important details...their use, visit our Antibody Guide . Browse or search the table below or visit the following pages to...Antibodies targeting proteins for neuroscience research. Institute for Protein Innovation Collection :...

...resources relevant to these organisms, or use our Search page to look for specific genes or applications...Browse Plasmids by Microbe System Plasmids for Archaea Species Haloferax sp. Methanococcus maripaludis...External Resources European Saccharomyces Cerevisiae Archive for Functional Analysis (EUROSCARF) (Link opens...

...of posttranslational modifications in cells and research into its many roles in protein function and signaling...Söll labs at Yale University changed the way researchers can explore important questions surrounding serine...for testing phosphoproteomics methods, proteomics search algorithms, proteomics phosphosite localization...