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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 435 results for: erg

Showing: 101 - 120 of 435 results

  3. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ...their viruses; food allergy labs use standard curves with ELISAs to measure allergen concentrations in ...What do a viral vector production facility, food allergy testing lab, and the grad student down the hall...

  4. An Introduction to Adenovirus

    Type
    Blog Post
    ... zoonotic? A systematic review of the evidence. Emerg Microbes Infect, 8(1), 1679–1687. https://doi.org...of a Cytopathogenic Agent from Human Adenoids Undergoing Spontaneous Degeneration in Tissue Culture. Proc...M., Foresythe, A., Huh, H. J., Bazinet, A. L., Bergman, N. H., Bull, R. L., Garcia, K. Y., Goodrich, J...

  5. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...Pigmentosa. I quickly became fascinated by this emerging technology and its potential for the treatment...cost of clinical development. As a result, Luk Vandenberghe and colleagues at MEEI co-founded a non-profit...approves gene therapy for blindness 2. Gene Therapy emerges from disgrace to be the next big thing, again 3...

  6. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...third chromosome, ten base pairs in length. An emerging family of sequencing techniques function as a ...coordinates into meaningful experiments. Table 1: An emerging family of "GPS for the genome" techniques Technique...going. Appendix DNAse Hypersensitive Sites and Intergenic CRISPRs Yours truly generated the DHS data set...

  7. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...the earliest uses of GFP. Fluorescence resonance energy transfer (FRET): This is used to study the interactions...proteins or between two domains of a protein that undergoes conformational change. Typically, two fluorescent...come into close proximity, the two halves of EGFP undergo folding, maturation, and fluorescence. Biosensors...

  8. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...sensitivity and detection - since they frequently undergo conformational changes after target binding, aptamers... biosensors. Aptabeacons Fluorescence resonance energy transfer (FRET) technology has been studied and... L. “Aptamer based electrochemical sensors for emerging environmental pollutants.” Front Chem. 2014; 2...

  9. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ... was designed in response to requirements that emerged from intensive engagement with a wide range of ...research computing. The two guiding principles emerging from the engagement with the Wisconsin research...fundamental driver of these principles and has emerged as a byproduct of connectivity. Between 2012 and...

  10. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...molecules of an RNA species with high specificity (Gootenberg et al., 2017). This system, dubbed SHERLOCK (...Find plasmids from this paper at Addgene.  3. Gootenberg, Jonathan S., et al. "Nucleic acid detection ...plasmids from this paper at Addgene. 6. Shmakov, Sergey, et al. "Discovery and functional characterization...

  11. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...fashion while allowing for creative solutions to emerge. They enjoy the fact that each new challenge will...amount of time can sometimes become cauldrons on the verge of boiling. On other occasions, clients can be excessively...thoughtful planning and engagement, leaving mental energy to enjoy the more rewarding and educational parts...

  12. All Antibodies

    Type
    Collection
    ...recombinant antibodies. These monoclonal antibodies undergo application-specific validation and quality control...

  13. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...approach, Zhang et al. turned to the CRISPR/Cas9 Synergistic Activation Mediators (SAM) system available from...Young WB, Khalili K, Hu W. CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent...Wang Z, Pan Q, Gendron P, Zhu W, Guo F, Cen S, Wainberg MA, Liang C. CRISPR/Cas9-Derived Mutations Both...

  14. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...In vitro transcription none, need Cas9 plasmid Sternberg 177783 L4440_BioBrick-sgRNA T7 BbsI, BsaI Ingestion...

  15. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...experiments (Rolland et al., 2016; Su et al., 2004; Taschenberger et al., 2017).     Figure 3: Comparison...C. M., Cheng, F., Qiu, J., Carette, J. E., & Vandenberghe, L. H. (2018). An Alternate Route for Adeno-.... https://doi.org/10.1007/s11064-004-6881-1 Taschenberger, G., Tereshchenko, J., & Kügler, S. (2017). ...

  16. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...there is a lack of expert mentorship during undergraduate and graduate studies. Students also need more... a rapidly changing, globalized environment re-emerging from international financial struggle, our new...mentorship in the field of Biomedicine to 78 undergraduate/graduate students from 5 independent universities...

  17. Synthetic Biology - Overview

    Type
    Collection
    ...of community-created BioBricks JBEI - Joint Bio-Energy Institute Synberc - Synthetic Biology Engineering...

  18. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...delivery methods, but since TEs insert mostly in intergenic regions, gene disruption is less of a concern...to a single location in bacterial genomes, the Sternberg lab developed the INTEGRATE (Insertion of Transposable...transposons-the-jumping-genes-518/   Sandoval-Villegas N, Nurieva W, Amberger M, Ivics Z (2021) Contemporary Transposon Tools...

  19. Important Considerations When Using AAVs

    Type
    Blog Post
    ...has been demonstrated that certain serotypes can undergo axoplasmic transport. These AAVs may move toward...Examining serotypes 1, 8 and 9, not only do they undergo retrograde and anterograde transport, but they ...avoid this rate limiting step and can immediately undergo replication and transcription. However, with scAAV...

  20. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...both methods did a more comprehensive gene list emerge that accurately reflected the scope of genes known... straightforward positive selection of plants undergoing RNAi, and LIIbeta F 1-2 RNAi, which allows assembly...microscopic plants must be visually identified as undergoing RNAi through the fluorescent reporter and manually...
Showing: 101 - 120 of 435 results