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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 363 results for: gne

Showing: 101 - 120 of 363 results

  1. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...created the sensor that you need. FRET biosensors designed to measure specific small biomolecules or gene...biosensor, Cameleon (Miyawaki et al., 1997), was designed to measure intracellular calcium and published...

  2. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...forever. microPublications are peer-reviewed and assigned a DOI that can be referenced. In addition the ...continuously curated as we continue to receive plasmids designed for the worm so check back frequently for what...

  3. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ... Fragmid is a modular vector assembly process designed for a quick, plug-and-play approach to cloning...sequence. This means that each component can be designed with an overhang specific to the type of module...

  4. Design Tips for Prime Editing

    Type
    Blog Post
    ...and PE5b: In this approach, the nicking sgRNA is designed to bind only after the edit is installed. This...approach. Figure 7: Nicking sgRNAs can be designed to target edited DNA, so that nicking only occurs...

  6. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...individual sequencing reads (~150 bp each) are aligned to the reference sequence of the plasmid used to...nucleotide collection database, and it may also be aligned to plasmids in our inventory containing the suspected...

  8. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...that’s suitable for PCR amplification using pre-designed primers. By using this library to infect an animal...isolate DNA from the infected animal and use the pre-designed primers to determine which version(s) of the capsid...

  11. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ... main requirement for IVA cloning is carefully designed primers. The majority of mistakes are due to incorrect... bind at either side of the undesired portion, designed to amplify the rest of the vector sequence. Encode...

  12. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...-specific primers: Insert-specific primers are designed to anneal to an insert-specific sequence. This...design backbone-specific primers. These primers are designed to anneal to sites that flank the insert site....

  14. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...such techniques can be problematic, however. Well designed aptamers can help reduce such background, thereby...temperature than its Spinach ancestors, and has a lower magnesium dependence. Though initially still unstable in...

  15. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...from Streptococcus thermophilus and a rationally-designed truncated Cas9. Unfortunately, certain drawbacks...J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brötzmann J, Staahl BT, Taylor KL, Desmarais...

  16. Bioinformatics at Addgene

    Type
    Blog Post
    ...each week. Our internal software was originally designed to help us work in large formats, such as 96-well...alone! The benefits of containers are they are designed to ‘contain,’ in a complete package, all of the...

  17. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...microbial survival despite exposure to antibiotics designed to kill them or to impede their growth. When we...equipment, etc. Virtually any preventive measure designed to help keep you from getting sick can be helpful...

  18. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...CRISPR/Cas9 system floating around, and many pre-designed plasmids containing these variants ready for purchase...desired edit. Good news! Remember the primers you designed to generate your pCAG-EGXXFP plasmid? They are...

  20. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...and some of the most popular commercial strains designed to avoid them. Why do I need an expression strain...Proteins: The most common purification protocols are designed for soluble, cystosolic protein products, but ...
Showing: 101 - 120 of 363 results