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TypeBlog Post...Northeastern University in Boston, MA. He obtained his BSc and MSc in Plant Biotechnology from Leibniz Universität... Universität Hannover (Germany), before starting his work in the Lee-Parsons Lab. References D'Aoust...below. The GUS gene (β-glucuronidase) with histochemical GUS-staining is a very useful visual reporter...
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PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog Post...editing experiments come into play. Nicolas Mathis received his PhD from Gerald Schwank's lab at the University...developed a strong passion for computational biology. His expertise includes applying machine learning techniques...efficiencies, we could train our prediction model PRIDICT (Mathis & Allam et al., 2023). We trained our models in...training led to the recent development of PRIDICT2.0 (Mathis et al., 2024). The additional datasets used in ...pegRNA editing efficiencies for specific targets (Mathis & Allam et al., 2023, 2024; Yu et al., 2023; Koeppel...variations in chromatin context. Our recent studies (Mathis et al., 2024) and concurrent research by the Shendure...Cell. https://doi.org/10.1016/j.cell.2024.03.020 Mathis, N., Allam, A., Kissling, L., Marquart, K. F., ... -
New Podcast Segment: Hot Plasmids
TypeBlog Post...from John Chodera's Lab - Get high expression of His-tagged human kinases in bactera. Listen to Our Segment... -
Streaking for Single Colonies: The Streak Plate Challenge
TypeBlog Post.... So when Dave Westenberg taught this concept in his microbiology lab course, he decided to add a bit ... -
Congratulations, Deck The Lab winners!
TypeBlog Post...Matthijs Dorst’s Deck the Lab Nativity scene below his Christmas tree! It is that time of the year again... -
A Scientist's Guide to Ebola
TypeBlog Post...coverage on the subject in the New York Times and on his blog, where he has offered to answer readers' questions... -
CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog Post...in Molecular Plant Sciences since December 2020. His research focuses on the efficiency of transposon-... with low efficiency. Sheng Yang obtained his Ph.D. from Shanghai Institute of Biochemistry, Chinese... -
CRISPR Activation: A Practical Guide
TypeBlog Post...member of the Addgene Scientific Advisory Board. His laboratory at Columbia University is focused advancing...the creation of innovative technologies. To date his group has develop several popular CRISPR-based tools... -
MassBioEd Career Exploration Day at Addgene
TypeBlog Post...Scientist Jessica Welch showed some highlights of his travels from 2015, including a great European photo... -
Science Communication for Everyone
TypeBlog Post...minorities to study STEM subjects. Here, Sadangi and his team answer a few questions about The Addictive Brain... -
Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies
TypeBlog Post...school, I came across Joseph Campbell, and read about his "follow your bliss' mantra and never forgot it. Usually... -
Cre-lox system
TypeCollection...Mammalian Sato 123128 pET-His-Cre 6xHis-Cre T7 Bacterial Liu 123131 pET-His-VCre 6xHis-VCre T7 Bacterial Liu...9xGGS-Cre-6xHis Cre fused to highly cationic GFP variant T7 Bacterial Liu 62939 pET-Cre-6xHis Cre-6xHis T7 Bacterial...Nxph4 Mammalian Zeng 62372 pET-(-30)GFP-9xGGS-Cre-6xHis Cre fused to supernegatively charged GFP variant...; Targeting vector for Pax7 Tanaka 111187 pBADZ-HisCre Cre; arabinose inducible. PBAD promoter Bacterial... -
Microbes: Look, Listen, and Tell
TypeBlog Post...methods for cellular imaging with ultrasound. In his research he applies naturally occurring bacterial... -
Editor's Choice, September 2016
TypeBlog Post...out our interview with Michael Koeris to learn how his company uses phage to test for Listeria in food products...surpassed 60,000 views for the month of September! Historically we do better in September than in the summer... -
CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats
TypeBlog Post... the Genetic Perturbation Platform at the Broad. His mom always told him that he has the best words and... -
Editor's Choice, August 2016
TypeBlog Post...techniques researchers are using. The detailed results of his survey can be found in the full post but TLDR: many... -
IDP and your PI: A Roadmap for Career Planning and Personal Development
TypeBlog Post...supplement of this paper), help each person organize his or her thoughts to facilitate honest conversation... -
"What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring
TypeBlog Post...not just to share your riches but to reveal to him his own." -Benjamin Disraeli Mentor, Sponsor, Advisor... -
Embracing Serendipity: A Crucial Element in the PhD Journey
TypeBlog Post..." He successfully transitioned to industry after his PhD and a couple of postdocs focusing on molecular... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog Post...the National Academy of Sciences in March, he and his colleagues took another important step forward by...