We narrowed to 322 results for: phy
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TypeBlog Post...degradation. Despite how easy these experiments are to physically set up in the lab, RNAi is often completely ineffective...can be used to carry out any biochemical and biophysical study involving protein stability or binding ...
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Viral Vectors 101: Biosensors
TypeBlog Post...of applications within chemistry, biology, and physics, in this blog we will focus on those which can ...monitor the fundamental cellular building blocks. Physiological factors Unlike the previously described sensors... -
3 Challenges in Plant Synthetic Biology
TypeBlog Post...biology at Virginia Tech, and earned a Ph.D. in Biophysics from UC Davis in 2007. Nikolai trained as a post-doc...ocean. After receiving her bachelors in Molecular Biophysics and Biochemistry from the Illinois Institute ... -
CRISPRainbow and Genome Visualization
TypeBlog Post... The technique successfully resolved loci with physical map distances of 2 and 75 Mbp, with the calculated...distances correlating with the previously established physical map. In comparing pairs of targets ~2 Mbp apart... -
RaPID Detection of RNA-protein Interactions
TypeBlog Post...present in the cell, it can also introduce non-physiological RNA-protein interactions due to sample processing...t be used to study endogenous RNAs at their physiological expression levels; however, knock-in of BoxB... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post... ligase, is the basis of TRIM-away. In normal physiology, TRIM21 recruits ubiquitin and the proteasome...enable future researchers to better simulate physiological gene expression regimes that cannot be easily... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog Post...low-throughput methods such as patch-clamp electrophysiology, a technique that’s used to measure electric...selected for characterization with patch clamp electrophysiology. All thirty were functional and their measured... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog Post...art with colorful microbes? Try your hand at photography and participate in a science photo competition...Indonesia. Since then, I have tried many things: photography, making videos, presenting in pubs and at science... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post... tension microscopy (MTM), which could monitor physical stresses or even apply forces (Gayrard and Borghi... Biosensors and Intravital FRET Microscopy." Biophysical Journal 111.6 (2016): 1103-1111. PubMed PMID:... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog Post...Biosciences (UCIBIO) focused on understanding the physiological and pathological roles of human cell glycans.... Her main work focuses on unraveling the pathophysiological role of glycans. Follow her on Facebook: ... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog Post...bioproduction of therapeutic methylxanthine drugs like theophylline. To facilitate future work with this goal, the...peak of 519 nm. These proteins have improved photophysical properties and can be used in quenching, FRET... -
Hot Plasmids - October 2020
TypeBlog Post... brain. But studying synaptic connections and physiology is particularly challenging as they are great...target the synaptic scaffolding proteins PSD95 and gephyrin to label excitatory and inhibitory synapses, respectively... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...fluorescence complementation analysis under physiological conditions. Shyu YJ, Liu H, Deng X, Hu CD. Biotechniques... -
Viral Vectors 101: Virus Safety
TypeBlog Post...encoded in the transfer plasmid, the component that physically integrates into the host genome, the virus is...caution as they are integrating viruses – they physically deposit their cargo into the host’s DNA. These... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog Post...biology at Virginia Tech, and earned a Ph.D. in Biophysics from UC Davis in 2007. Nikolai trained as a post-doc...ocean. After receiving her bachelors in Molecular Biophysics and Biochemistry from the Illinois Institute ... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog Post... molecular biology-grade agarose containing physiological concentrations of salts and glucose. If we circled... terms of growth rates, morphology or other physiological properties. An advantage of this technique is... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog Post...biological microscopy." American Journal of Physiology-Cell Physiology 300.4 (2011): C723-C742. PubMed PMID:... -
Viral Vectors 101: Voltage Indicators
TypeBlog Post... do things like translate perceived signals to physical actions. Understanding neuronal pathways and the...membrane, and this voltage is particularly physiologically relevant in neurons, myocytes, and endocrine... -
Google Forums Round Up: First Impressions of NgAgo
TypeBlog Post...requires incubation at 55 °C - a dangerous, non-physiological temperature for mammalian cells and one which...things (101 lessons on Spanish, C++, guitar, photography) and often dreams of a sunny day in Edinburgh... -
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy
TypeBlog Post...example), as this can also be perturbed by supraphysiological expression levels. BiFC quantification This... amenable to studying PPIs under relatively physiological conditions when compared to FRET. However, it...