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We narrowed to 733 results for: son

Showing: 101 - 120 of 733 results

  3. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...Anti-Copper ATPase 2 (Wilson's disease protein) [L62/29] Copper ATPase 2 (Wilson's disease protein) Human...

  4. CRISPR Guide

    Type
    Guide
    ... A., Chan, M. M., Bauer, D. E., Marson, A., Parsons, L. R., & Adamson, B. (2024). Improving prime editing...including transposons, integrases, and recombinases, with Cas enzymes. CRISPR Transposases Transposon systems...I., Yoon, Y., Song, C., Cao, Y., Gallant, J., Xue, W., Rivera-Pérez, J. A., & Sontheimer, E. J. (2019)...S., Cofsky, J. C., Kranzusch, P. J., Sontheimer, E. J., Davidson, A. R., Maxwell, K. L., & Doudna, J. ..., J., Edraki, A., Shah, M., Sontheimer, E. J., Maxwell, K. L., & Davidson, A. R. (2016). Naturally occurring... Chen, C., Nelson, J. W., Newby, G. A., Sahin, M., Osborn, M. J., Weissman, J. S., Adamson, B., & Liu,...Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., Pogson, A. N., Kim, J. Y., Chung, A., Leonetti...

  5. Molecular Cloning Techniques

    Type
    Guide
    ...exonuclease. Read more in our Gibson Assembly blog post . Figure 4: Summary of Gibson assembly. Created with ...Cloning Techniques Restriction Enzyme Gateway TOPO Gibson Golden Gate & MoClo Ligation Independent Cloning...cost, availability of starting materials, or just personal preference. Check out our blog on choosing the... = topoisomerase I. Created with BioRender.com. Gibson Assembly (Isothermal Assembly Reaction) Isothermal...Isothermal cloning, more commonly known as Gibson assembly, takes advantage of the properties of three common...enzymes: 5' exonuclease, polymerase, and ligase. In Gibson assembly, DNA fragments with 20–40 base-pair homology...to create one piece of dsDNA. A major benefit of Gibson cloning is that it allows for the assembly of multiple...

  6. Optogenetics Guide

    Type
    Guide
    ...t/t), ChrimsonR Red-shifted peak action spectra Examples: VChR1, C1V1(t/t), Chrimson, ChrimsonR, Chronos...helveticum 500 Chrimson, ChrimsonR Red-light drivable channel from Chlamydomonas noctigama . ChrimsonR carries...research, helping scientists to better understand Parkinson’s disease, depression, drug addiction, and more...Ramakrishnan C, Mattis J, Prakash R, Diester I, Goshen I, Thompson KR, Deisseroth K. 2010 Molecular and cellular ...Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK, Morimoto TK, Chuong AS, Carpenter EJ...Methods. 9(2):159-72. PMID 22179551 Saunders A, Johnson CA, Sabatini BL 2012. Novel recombinant adeno-associated...21363959 Yizhar O, Fenno LE, Prigge M, Schneider F, Davidson TJ, O'Shea DJ, Sohal VS, Goshen I, Finkelstein...

  7. Antibody Guide

    Type
    Guide
    ...using sonication to break DNA up into fragments of 300-1000 bps in length. Note: This sonication process...may need additional processing steps, such as sonication. Denature proteins, using heat and/or chemicals...housekeeping genes). This allows for relative comparison of expression between different samples, by normalizing...normalizing protein expression to the controls before comparison. Alternatively, the samples can be normalized...ChIP protocols use enzyme digestion instead of sonication. This approach is gentler but results in non-...isolated and analyzed. Validation relies on size comparison of peptides. Independent antibody validation ...

  8. Chemogenetics Guide

    Type
    Guide
    ...: References and Further Reading Atasoy, D., & Sternson, S. M. (2018). Chemogenetic Tools for Causal Cellular...P. H., Atasoy, D., Su, H. H., Looger, L. L., & Sternson, S. M. (2011). Chemical and genetic engineering...Ramirez MH, Hu X, Galvan A, Basu J, Michaelides M, Sternson SM (2019). Ultrapotent chemogenetics for research...doi.org/10.1117/1.NPh.11.2.024208 PMID: 38559366 Sternson, S.M., Roth, B.L. (2014). Chemogenetic tools to...doi.org/10.1038/srep14366 PMID: 26399324 Vardy, E., Robinson, J. E., Li, C., Olsen, R. H. J., DiBerto, J. F...A., Pleil, K. E., Mazzone, C. M., Mosier, P. D., Song, J., Kash, T. L., Malanga, C. J., Krashes, M. J....

  9. Adenovirus Guide

    Type
    Guide
    ... cloning methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may... Bullard, B. L., Corder, B. N., Gordon, D. N., Pierson, T. C., & Weaver, E. A. (2020). Characterization...Asiedu, C., Geisbert, J., Stanley, D., Honko, A., Johnson, J., Mulangu, S., Pau, M. G., Custers, J., Vellinga...gene and cell-based therapies in the new era of personalized medicine . Genes & Diseases, 4 (2), 43–63. https...window) Luo, J., Deng, Z. L., Luo, X., Tang, N., Song, W. X., Chen, J., Sharff, K. A., Luu, H. H., Haydon...

  10. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...Transposagen What is the piggyBac™ transposon? The piggyBac™ transposon was originally identified in moths...until 2005 [1]. Like other transposons, piggyBac™ has two components, a transposon and a transposase. The ...titrating the transposase to transposon ratios. High ratios of transposase to transposon result in a greater number...ability to distribute plasmids with the piggyBac™ transposon. These plasmids, when combined with a source ...you to quickly transfer a DNA sequence from the transposon vector to one of many TTAA sequences distributed...genome. We encourage you to deposit your piggyBac™ transposon vectors with us to help us expand this useful...transposase facilitates the integration of the transposon specifically at ‘TTAA’ sites randomly dispersed...

  11. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...important for social and regulatory reasons. 1.) The transposon consists of a mirrored set of inverted....   The Sleeping Beauty transposon system The Sleeping Beauty (SB) transposon system is one such system...Transposons are sequences of DNA that can move around in a genome. In a laboratory setting, transposons...the site of insertion. In both of these cases, transposons combine the advantages of viruses and naked DNA...mechanisms. The use of non-viral vectors, like transposons, avoids many, though not all, of these defenses...reaction occurs which cuts the DNA.3.) The released transposon can now bind a strand of DNA with a TA dinucleotide...plasmid is empty following the removal of the transposon; the plasmid is then degraded by the cell.4.)...

  12. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...9d0855a1b533" %}{% module_attribute "button_text" is_json="true" %}{% raw %}"Visit Addgene's Data Hub"{% ...end_module_attribute %}{% module_attribute "child_css" is_json="true" %}{% raw %}{}{% endraw %}{% end_module_attribute..._attribute %}{% module_attribute "css" is_json="true" %}{% raw %}{"border-radius":"10px","padding-left...end_module_attribute %}{% module_attribute "definition_id" is_json="true" %}{% raw %}null{% endraw %}{% end_module_attribute..._attribute %}{% module_attribute "field_types" is_json="true" %}{% raw %}{"button_text":"text","link":...end_module_attribute %}{% module_attribute "label" is_json="true" %}{% raw %}null{% endraw %}{% end_module_attribute...end_module_attribute %}{% module_attribute "link" is_json="true" %}{% raw %}{"no_follow":false,"open_in_new_tab...

  13. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...recruits other silencing proteins. Figure 1: Comparison of shRNA and miRNA processing and silencing pathways...genomic sequences (Figure 2B).   Figure 2: A comparison of an shRNA plasmid sequence and an miRNA plasmid...14305620e565" %}{% module_attribute "button_text" is_json="true" %}{% raw %}"Browse Addgene's shRNA plasmid...end_module_attribute %}{% module_attribute "child_css" is_json="true" %}{% raw %}{}{% endraw %}{% end_module_attribute..._attribute %}{% module_attribute "css" is_json="true" %}{% raw %}{"border-radius":"10px","padding-left...end_module_attribute %}{% module_attribute "definition_id" is_json="true" %}{% raw %}null{% endraw %}{% end_module_attribute..._attribute %}{% module_attribute "field_types" is_json="true" %}{% raw %}{"button_text":"text","link":...

  14. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type
    Blog Post
    ...fcd590559fc0" %}{% module_attribute "button_text" is_json="true" %}{% raw %}"Browse the Allen Institute's...end_module_attribute %}{% module_attribute "child_css" is_json="true" %}{% raw %}{}{% endraw %}{% end_module_attribute..._attribute %}{% module_attribute "css" is_json="true" %}{% raw %}{"border-radius":"10px","border-style...end_module_attribute %}{% module_attribute "definition_id" is_json="true" %}{% raw %}null{% endraw %}{% end_module_attribute..._attribute %}{% module_attribute "field_types" is_json="true" %}{% raw %}{"button_text":"text","link":...end_module_attribute %}{% module_attribute "label" is_json="true" %}{% raw %}null{% endraw %}{% end_module_attribute...end_module_attribute %}{% module_attribute "link" is_json="true" %}{% raw %}{"no_follow":false,"open_in_new_tab...

  15. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ...a7049139e11d" %}{% module_attribute "button_text" is_json="true" %}{% raw %}"Find OCaMP plasmids available...end_module_attribute %}{% module_attribute "child_css" is_json="true" %}{% raw %}{}{% endraw %}{% end_module_attribute..._attribute %}{% module_attribute "css" is_json="true" %}{% raw %}{"padding-left":"200px","padding-right...end_module_attribute %}{% module_attribute "definition_id" is_json="true" %}{% raw %}null{% endraw %}{% end_module_attribute..._attribute %}{% module_attribute "field_types" is_json="true" %}{% raw %}{"button_text":"text","link":...end_module_attribute %}{% module_attribute "label" is_json="true" %}{% raw %}null{% endraw %}{% end_module_attribute...end_module_attribute %}{% module_attribute "link" is_json="true" %}{% raw %}{"no_follow":false,"open_in_new_tab...

  16. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    ...but he or she probably has reasons for it. Do not take criticism personally or get defensive. Look at ...important issues in person or over the telephone instead of email. As you cannot see a person’s body language...get nods from nearly every participant. Robert Bramson, author “Coping with Difficult People,” identified...specific strategies to deal with each of these personality types is beyond the scope of this article, there...resolve conflicts What Is Assertiveness? An assertive person is able to communicate their ideas confidently ...your supervisor, even if he or she is a difficult person: 1) Understand your supervisor’s expectations ...or she would like a written report or to meet in person.   Regardless of your supervisor’s management style...

  17. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...were usually the products of teams: Watson, Crick, & Franklin, Meselson-Stahl, Hershey-Chase...Who was I ...intelligent, motivated person really could know a little bit about everything or in Jefferson’s case, even a lot...work of more than one person. A selfish part of you might not want another person’s name on your next paper...clear why that person is there. If it becomes clear after a few meetings that a person is neither adding...all our externally facing kit pages. No single person could possibly understand every nuance of how kits...should have a goal, or at the very least, a clear reason for existing. Here are a few types of teams that... - with the possible exception of when Thomas Jefferson dined alone.” There was a time in human history...

  18. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...to the conclusion that a conflict is personal (”Person A and Person B just don’t get along”) when in fact...conflict are substantive (“Person C told Person A one thing and inadvertently told Person B something else.")...also be categorized as either “personal” or “substantive”. A personality-based conflict occurs when two...multiple channels is not reasonable or sustainable. That said, when you know that a person or team should be ...large organization, because confirming that every person has read and understood every communication that...assign the responsibility in question to a specific person or team. What Makes You a Good Conflict Resolver... for a fight. But you also can’t be the type of person who avoids conflict at all costs or actively runs...

  19. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ... result of the necessary restrictions on close personal contacts. However, that should not deter any student... letter can be found on the Addgene blog.   3. Personal statement brilliance In addition to sending in... cover letter, you are always asked to draft a personal statement, which is the perfect opportunity to...questions, thus devoting some time to writing a solid personal statement will pay dividends. This is the only...and forward-looking. Embrace it, and let your personality leap out of the page. Finally, as previously ...find the tools that can help you craft a solid personal statement.  4. Letters of recommendation wisdom...to vouch for the character you painted in your personal statement. Make certain to choose your recommenders...

  20. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... cloning methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may...Ravin, S. S., Su, L., Theobald, N., Choi, U., Macpherson, J. L., Poidinger, M., Symonds, G., Pond, S. ...Ridder, J., Allahyar, A., Wang, W., Carpenter, E., Mason, A., Wong, G. K., Cameron, E. R., Kilbey, A., & ...Srivastava, S., Hussain, R., Banani, M. A., Day, W., Stevenson, E., Madigan, M., Chen, J., Khinder, R., . . ....
Showing: 101 - 120 of 733 results