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TypeCollection...zebrafish, mouse, rat, and human. Developed by the Stanley Qi Lab . CCTop - CRISPR/Cas9 target online predictor...Link opens in a new window) A Google groups forum started by the Feng Zhang Lab . Protocols Lab(s) Description...
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Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog Post...replicate while maintaining the undifferentiated “stem” state and, Their potency or capability to differentiate...cells found in the zygote through to the morula stage are totipotent [2-4]. Pluripotency: When a cell ... -
Welcome to the Lab Bench! New Protocol Series Helps Trainees Get Started in the Lab
TypeBlog Post...informative introduction for those who are just starting their careers in the lab and even those who might... -
Retrograde AAV viral preps
TypeCollection...transgenes in Cre transgenic lines. As part of our standard viral production , these viral vectors undergo... plasmid with PCR, purity assessment by silver staining, in vitro and (when possible) in vivo testing,... -
Allen Institute for Brain Science AAV Enhancer Collection
TypeCollection...AiE0410m Cre Oligodendrocytes Whole Brain * = Substantial non-specific labeling observed Publications Ben-Simon...Daigle, T. L., Dwivedi, D., Way, S. W., Oster, A., Stafford, D. A., Mich, J. K., Taormina, M. J., Martinez... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post... panels) equipped with destabilized GFP calcineurin reporter (blue) and stained with PI (pink) to indicate...Image from Isaacman-Beck et al., 2019. Get started with sparse labeling Isaacman-Beck et al., bioRxiv...bioRxiv 2019. https://doi.org/10.1101/788679 A destabilized GFP reporter for calcineurin activity in yeast...transcriptional reporter consisting of a quickly-degraded destabilized GFP variant fused to a 4X CDRE repeat. In addition... that this method is compatible with dead cell staining using propidium iodine (PI), allowing for calcineurin...Truncated TALE-fluorescent protein fusions as a DNA staining dye Article contributed by Jennifer Tsang ...generated fluorescent protein fusions with tTALE to “stain” DNA. TALE proteins can bind specific DNA sequences... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog Post...their promising research projects. Understanding drug resistance mechanisms of Mycobacterium tuberculosis...interested in understanding the mechanisms behind Mycobacterium tuberculosis resistance to pyrazinamide...targets. An early and accurate detection of PZA-resistance is necessary to improve tuberculosis care and...Sheen aims to identify new genes related to PZA-resistance by using different approaches, including CRISPR...Her research should provide us with a better understanding of PZA action and could potentially stimulate...diagnostic methods and improved therapies for PZA-resistant Mycobacteria tuberculosis. Investigation of multispecies...this condition are urgently needed. Therefore, understanding neuronal networks, their interactions and information... -
Synthetic Biology - Browse Plasmids
TypeCollection...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse the list... -
Synthetic Biology - Algal
TypeCollection...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene'... -
Synthetic Biology - Bacterial
TypeCollection...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene'... -
Synthetic Biology - Fungal
TypeCollection...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene'... -
Synthetic Biology - Mammalian
TypeCollection...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene'... -
Synthetic Biology - Plant
TypeCollection...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene'... -
Synthetic Biology - Worm
TypeCollection...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene'... -
Synthetic Biology - Yeast
TypeCollection...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene'... -
Synthetic Biology - Sensing and Signaling
TypeCollection...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic... -
Synthetic Biology - Metabolism
TypeCollection...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic... -
Synthetic Biology - Networks and Gene Regulation
TypeCollection...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic... -
Zinc Finger Consortium Reagents
TypeCollection... Lab plasmids The Zinc Finger Consortium was established to promote continued research and development... -
Synthetic Biology - Cloning and Genomic Tools
TypeCollection...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic...