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We narrowed to 1,047 results for: sta

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Showing: 101 - 120 of 1047 results

  1. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...into two stages: Dedifferentiation - Conversion of adult somatic cells into the pluripotent state. Redifferentiation...differentiated somatic cell reverts back to a pluripotent state, which then proliferates and is redifferentiated...lineage, without dedifferentiating into a pluripotent stage. Cellular reprogramming The reversal of a differentiated...differentiated cell type to an undifferentiated state and then redifferentiation into the cell type of choice...differentiated cells of choice. The dedifferentiation stage involves overexpression of four reprogramming factors...differentiated somatic cell to revert back to a pluripotent stage (iPSC formation) [2, 3]. The iPSCs then proliferate...reprogrammed to iPSCs successfully [10-12]. The next stage of reprogramming consists of redifferentiation of...

  4. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    ... panels) equipped with destabilized GFP calcineurin reporter (blue) and stained with PI (pink) to indicate...Image from Isaacman-Beck et al., 2019. Get started with sparse labeling Isaacman-Beck et al., bioRxiv...bioRxiv 2019. https://doi.org/10.1101/788679 A destabilized GFP reporter for calcineurin activity in yeast...transcriptional reporter consisting of a quickly-degraded destabilized GFP variant fused to a 4X CDRE repeat. In addition... that this method is compatible with dead cell staining using propidium iodine (PI), allowing for calcineurin...Truncated TALE-fluorescent protein fusions as a DNA staining dye Article contributed by Jennifer Tsang ...generated fluorescent protein fusions with tTALE to “stain” DNA. TALE proteins can bind specific DNA sequences...

  5. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...their promising research projects.   Understanding drug resistance mechanisms of Mycobacterium tuberculosis...interested in understanding the mechanisms behind Mycobacterium tuberculosis resistance to pyrazinamide...targets. An early and accurate detection of PZA-resistance is necessary to improve tuberculosis care and...Sheen aims to identify new genes related to PZA-resistance by using different approaches, including CRISPR...Her research should provide us with a better understanding of PZA action and could potentially stimulate...diagnostic methods and improved therapies for PZA-resistant Mycobacteria tuberculosis. Investigation of multispecies...this condition are urgently needed. Therefore, understanding neuronal networks, their interactions and information...

  7. Synthetic Biology - Algal

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    Collection
    ...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene'...

  8. Synthetic Biology - Bacterial

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    ...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene'...

  9. Synthetic Biology - Fungal

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    ...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene'...

  10. Synthetic Biology - Mammalian

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    ...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene'...

  11. Synthetic Biology - Plant

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    ...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene'...

  12. Synthetic Biology - Worm

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    Collection
    ...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene'...

  13. Synthetic Biology - Yeast

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    Collection
    ...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Browse Addgene'...

  15. Synthetic Biology - Metabolism

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    Collection
    ...Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic...

  17. Zinc Finger Consortium Reagents

    Type
    Collection
    ... Lab plasmids The Zinc Finger Consortium was established to promote continued research and development...

  19. NETRF

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    Collection
    ... Broad Institute Identifying Altered Epigenetic States and Drivers in Intestinal Carcinoid and Pancreatic...
Showing: 101 - 120 of 1047 results