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Showing: 101 - 120 of 320 results

  2. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...biomolecules of interest e.g. DNA, RNA, and lipids. Still, these molecules can be indirectly visualized by...facilitates the choice of the proper site, although it still is worthwhile to generate several variants and check...

  5. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ... TALENs, and ZFNs). However, several challenges still remain, such as making gene edits completely seamless...researcher may subsequently kill off any cells that still contain the donor plasmid after excision by PBx,...

  6. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...ve reduced the chance of off-target effects, you still need to do your due diligence to ensure they are...here. For some applications, this is ok, but it still must be controlled for and acknowledged as a potential...

  7. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ...cells in the population, so some of the cells will still appear green despite having active Cas9 (see tip...site in the middle of the PCR product, you would still be able to identify the cleavage product. 3. The...

  8. CRISPR Guide

    Type
    Guide
    ...mismatches toward the 5′ end distal to the PAM often still permit target cleavage. Cas9 undergoes a second ...desired edit, as a large percentage of DSBs are still repaired by NHEJ. How do you determine that your...

  9. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...dependent on the promoter of the cre recombinase while still taking advantage of the robust expression via the...likely be less than that of viral expression but may still be sufficient for successful stimulation. Testing...

  11. Promoters

    Type
    Guide
    ... simpler than eukaryotic transcription bacteria still have complex systems of gene regulation, like operons...

  12. Sequencing Primers

    Type
    Guide
    ... sequencing primer" and "3' sequencing primer". Still not sure what primer you need? Email us at help@...

  13. Cloning

    Type
    Guide
    ...which pieces of DNA that share no end homology can still be fused together in a seamless manner. To accomplish...

  14. Optogenetics Guide

    Type
    Guide
    ...domains from two different ChRs. These ChR variants still function as light-gated, cation channels resulting...

  19. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ...holidays may be over, but signs of holiday spirit still linger on around the Addgene office as we peruse...
Showing: 101 - 120 of 320 results