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Showing: 121 - 140 of 772 results
  1. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...Primers for reference). Many sequencing cores have a list of common primers that can be requested for no additional...used by Addgene during quality control? Addgene lists the primers used to obtain each result above the...
  2. Chemogenetics Guide

    Type
    Guide
    ...17360345 (Link opens in a new window) Berglund K, Clissold K, Li HE, Wen L, Park SY, Gleixner J, Klein ME, Lu...29351511 (Link opens in a new window) Armbruster BN, Li X, Pausch MH, Herlitze S, Roth BL (2007). Evolving...) Guettier JM, Gautam D, Scarselli M, de Azua IR, Li JH, et al. (2009). A chemical-genetic approach to...Link opens in a new window) Vardy E, Robinson JE, Li C, Olsen RHJ, DiBerto JF, et al. (2015). A New DREADD...experiments in this species may be entirely due to back-metabolism of CNO to clozapine, demonstrating the need for...activity, and do not seem to have the same back-metabolism issues as CNO and are attractive alternatives...controlled with cell-type specific promoters. Table 4 lists some common promoters found in chemogenetic receptor...
  3. CRISPR Guide

    Type
    Guide
    ...Bartolomei, M. S., Ferguson-Smith, A., Li, D., Yang, L., Wu, Y., & Li, J. (2015). CRISPR-Cas9-Mediated Genetic...: 25494202 Liu, X., Zhang, Y., Chen, Y., Li, M., Zhou, F., Li, K., Cao, H., Ni, M., Liu, Y., Gu, Z., Dickerson...: 23287718 Cui, Z., Tian, R., Huang, Z., Jin, Z., Li, L., Liu, J., Huang, Z., Xie, H., Liu, D., Mo, H...., J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., Cradick, T....V., Nguyen, N. T., Zheng, Z., Gonzales, A. P. W., Li, Z., Peterson, R. T., Yeh, J. J., Aryee, M. J., &...29160308 Hess, G. T., Frésard, L., Han, K., Lee, C. H., Li, A., Cimprich, K. A., Montgomery, S. B., & Bassik...., Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt, R., Solley, S. ...
  4. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...infection and selection G. Safety H. References H.1 Published articles H.2 Web resources I. Appendix I.1 Sequence...Biosafety Guide . Back to Top H. References H.1. Published Articles Functional siRNAs and miRNAs exhibit ...
  5. Ligation Independent Cloning

    Type
    Protocol
    ...of T4 Pol in the following step. This may be accomplished by gel purification . Step 5: Create Insert ...
  6. Video Library

    Type
    Protocol
    ...community. Jane Hannon Our Marketing & Outreach Specialist Jane Hannon discusses her career path, current...
  7. Centrifugation

    Type
    Protocol
    ...speed and time (and temperature, if applicable) listed in your protocol. Pro-Tip Spin speed is often given...
  8. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... linker (see Figure 1). The key to the biosensor lies in pH: DsRed is relatively pH-insensitive, while...plasmids in a future blog post. No time to read? Listen to our hot plasmids segment on the Addgene Podcast...constructed. The authors then used GMAP assembly to establish a collection of over 30 promoters and 140 genes...characterize positive hits. This work has been published in Cell. The Moffat laboratory maintains a website...Carlson RCIscript-GoldyTALEN, pC-GoldyTALEN Tom Ellis pTAL5-BB, pTAL6-BB David Grunwald pCS2TAL3-DD,...Center has kindly constructed and deposited an unpublished set of Zika gene plasmids to Addgene’s repository... prototrophy, can also aid in designing self-establishing metabolically cooperating (SeMeCo) communities...
  9. Immunocytochemistry

    Type
    Protocol
    ...reproducibility and accelerates science. Here, we list the specific equipment, reagents, and methods that...
Showing: 121 - 140 of 772 results