We narrowed to 210 results for: cycs

1. Viral Vectors 101: Virus Safety

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   Blog Post

   ...meaning it can’t replicate and continue the infection cycle once inside the host. While the virus can still ...

2. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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   ...nourseothricin (clonNat), spectinomycin, streptomycin, tetracycline, combinations of the above, or other. High...

3. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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   ...Using the Tet-on system, his lab has created a tetracycline cas9 inducible knock-out system. His lab is ...

4. 6 Tips for Grant Writing

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   Blog Post

   ... to improve your application for the next grant cycle.  Many thanks to our Guest Blogger, Sean Mac Fhearraigh...

5. Academic vs. Industry Postdocs

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   ...industry postdoc will free you of the never-ending cycle of grant-writing that is academic life. Of course...

6. Hot Plasmids and Viral Preps - May 2021

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   ...where gRNA expression is induced by anhydrous tetracycline. Three new anti-CRISPRs were identified that...

7. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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   Blog Post

   ...stop emerging at night. An incubator with a light cycle makes for very predictable work! When flies first...

8. Simplify Cloning with in vivo Assembly

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   Blog Post

   ... different templates. All PCRs are run as an 18 cycle, 25 μl single-tube reaction. If you are synthesizing...

9. Course-Based Undergraduate Research Experiences (CUREs)

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   Blog Post

   ...work on malate dehydrogenase (MDH), a citric acid cycle enzyme he studied during graduate school. As a stable...

10. Better Dyeing Through Chemistry & Small Molecule Fluorophores

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    Blog Post

    ... tag (Figure 1c). Click chemistry (e.g., transcyclooctene–tetrazine)– Nonnatural amino acids can be ...

11. Plasmids 101: Colony PCR

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    ...dNTPs and then incubate with a standard PCR thermocycling program. One key difference is the plasmid DNA...

12. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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    ...researchers to study aspects of the EBOV replication cycle under BSL-2 conditions (4). What is a minigenome...

13. Hot Plasmids: Fall 2024

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    ...with Cas9 on an integrative plasmid containing tetracycline repressor TetR and resistance selection marker...

14. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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    ...hydra have asexual and sexual reproductive life cycles. One can inject plasmid DNA in the eggs to get ...

15. Bioinformatics at Addgene

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    Blog Post

    ...Now, we could iterate our development and testing cycle faster due to the ease of jumping back and forth...

16. CRISPR-based Genome Editing Systems For Editing in E. coli

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    Blog Post

    ... plasmids for multicopy integration. (B) Anhydrotetracycline (aTc) induces expression transposases leading...

17. Hot Plasmids - August 2020

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    Blog Post

    ...dCas9-VPR repressor in hPSCs is also driven by a doxycycline inducible promoter. The gRNA piggyBac plasmids...

18. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    Blog Post

    ... in the 80s and 90s, Joe was that kid who used recycled notebook paper, despite the fact that it was gray...

19. Fluorescent Proteins 101: Aptamer Fluorophores

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    Blog Post

    ...while aptamers are more stable and can go through cycles of denaturation/renaturation. In vivo assays ...

20. How to Write a Scientific Review Article

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    Blog Post

    ...is probably “a normal day.” The way I broke this cycle was to just start writing. A word of advice: when...