..., for example, can also be printed quickly in Madagascar. Platforms that allow for on-going creativity...

...Additionally, align a custom nucleotide sequence against a given sequence using BLAST2. See this blog post...

...for variants that infect the CNS while selecting against those that also infect the liver.    Fig...

...on our Viral Service (and then we crunched them again) to find the most requested lentivirus and AAV of...

...to methylation if the enzyme's recognition site (TCTAGA) is preceded by GA or followed by TC. As shown ...

... rules that I try to follow in my own research. Again, some of this may not apply or may not work for ...

...proteins inside a cell have a commercial antibody against them. There’s also a question about how specific...

...PTGs containing 8 gRNAs targeting 4 MAPK genes, again using rice protoplasts. Each pair of gRNAs targeted...

...like those pioneered by Ewen Mullins of Ireland’s Teagasc.  Plant biotechnology is full of potential, but...

...really helpful, perhaps you wouldn’t mind doing it again next month?” Some mentoring relationships grow naturally...

...3-100 per cell, a stabilized promoter buffered against the effects of copy number on reporter gene expression...

...constantly communicating with a tumor, proving once again that a tumor is always interacting with its surroundings...

...here at our new location to grow. We are hiring again and getting ready to embark on new exciting projects...

...full-length protein that you want to generate antibodies against, or a unique peptide sequence derived from this...

...localization of the hM4Di, we used primary antibodies against mCherry, and secondary antibodies coupled to nanogold...

...from a complex mix using affinity chromatography against an amino acid sequence, or tag, on the protein....

...found (typically RNA that the RNAi is designed against), RISC can repress translation and/or degrade target...

... a kinases in a body part of choice. Zhou et al again used the Raf-MEK-ERK signaling pathway to demonstrate...

...in r/molecularbiology.   Oh, to be young again! Addgenie Marcy (now our Sr. Director of Production...

...you!)  Amount of information and level of detail Again, this is an obvious one on the surface, but it’s...

...check the fragments size of your digestion on an agarose gel. If the digestion does not provide the fragments...

...of “Gender, Science, and Technology” in 2011 and again in 2014. Resources on the Addgene Blog Learn How...

...enable you to quickly BLAST your sequencing result against the expected sequence for quick verification, which...

...tested T-DDOGs with two mouse GFP reporter lines; again, TdTomato was seen only in cells with GFP, at a ...

...the parent cell population using the library, propagated it, separated it into roughly identical replicate...

...acquisition, storage, and utilization - and on self-propagation by reproduction. Both physiological processes...

...systems that bacteria and archaea use to protect against these foreign nucleic acids. Acr family members...3' NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional Cas9s..., Weinstein, J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., ...: 27984730 Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott, D. A., & Zhang, F. (2013). Genome ...

...ceases relatively quickly due to immune response against AdV. Importantly, recombination-competent adenovirus...

...and selectable markers that allow students to propagate the plasmids in either E. coli (ori and ampR) ...

...with an antibody. This can artificially select against PPIs that aren't stable outside the native cell...

...the 22nd International C. elegans meeting, I was again reminded of the extent that C. elegans researchers...

...multiple genes to generate a cellular phenotype. Again ABE8.20-m was the winner. It could simultaneously...

...deschloroclozapine (DCZ) shows promise as a CNO alternative (Nagai et al., 2019). Regardless of which ligand you choose...

...., Mimee, M., Chang, E. B., Ruscheweyh, H.-J., Sunagawa, S., Mclellan, S. L., Willis, A. D., Comstock,...

...from your membrane, allowing you to probe the blot again, almost like new. This approach preserves your sample...

... is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat...

...of ensuring viral production is to perform PCR against components of the viral vector using the harvested...

... of the PAM may give Cpf1 the ability to cleave again and possibly mediate HDR in a “second chance” mechanism...

...desribed how P. furiosus has systems to defend against both DNA and RNA viruses - 2 cas systems regulate...

...fluorescent units and so lowers the FRET signal back again. The Ting lab constructed 2 versions of this reporter...