We narrowed to 771 results for: LIS
-
TypeProtocol...Antibody Validation Using the Indirect ELISA Method Run an indirect ELISA against a purified antigen to demonstrate...
-
CRISPR Guide
TypeGuide...Bartolomei, M. S., Ferguson-Smith, A., Li, D., Yang, L., Wu, Y., & Li, J. (2015). CRISPR-Cas9-Mediated Genetic...: 25494202 Liu, X., Zhang, Y., Chen, Y., Li, M., Zhou, F., Li, K., Cao, H., Ni, M., Liu, Y., Gu, Z., Dickerson...: 23287718 Cui, Z., Tian, R., Huang, Z., Jin, Z., Li, L., Liu, J., Huang, Z., Xie, H., Liu, D., Mo, H...., J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., Cradick, T....V., Nguyen, N. T., Zheng, Z., Gonzales, A. P. W., Li, Z., Peterson, R. T., Yeh, J. J., Aryee, M. J., &...29160308 Hess, G. T., Frésard, L., Han, K., Lee, C. H., Li, A., Cimprich, K. A., Montgomery, S. B., & Bassik...., Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt, R., Solley, S. ... -
Protocol - pLKO.1 – TRC Cloning Vector
TypeProtocol...infection and selection G. Safety H. References H.1 Published articles H.2 Web resources I. Appendix I.1 Sequence...Biosafety Guide . Back to Top H. References H.1. Published Articles Functional siRNAs and miRNAs exhibit ... -
Protocol - How to Create a Bacterial Glycerol Stock
TypeProtocol... bacteria for a longer time, you will need to establish glycerol stocks. The addition of glycerol stabilizes... -
Protocol - Over-Agar Antibiotic Plating
TypeProtocol...above result represents a single experiment. For publishable data, the experiment would need to be repeated... -
Protocol - How to Purify DNA from an Agarose Gel
TypeProtocol... in other lanes contaminating your sample. To accomplish this, it is best to skip lanes between samples... -
Protocol - How to Perform a Diagnostic Digest
TypeProtocol...restriction sites are present in a given sequence. For a list of the commonly used commercially available restriction... -
Ligation Independent Cloning
TypeProtocol...of T4 Pol in the following step. This may be accomplished by gel purification . Step 5: Create Insert ... -
Video Library
TypeProtocol...community. Jane Hannon Our Marketing & Outreach Specialist Jane Hannon discusses her career path, current... -
Centrifugation
TypeProtocol...speed and time (and temperature, if applicable) listed in your protocol. Pro-Tip Spin speed is often given... -
27 Hot Plasmids from 2016
TypeBlog Post... linker (see Figure 1). The key to the biosensor lies in pH: DsRed is relatively pH-insensitive, while...plasmids in a future blog post. No time to read? Listen to our hot plasmids segment on the Addgene Podcast...constructed. The authors then used GMAP assembly to establish a collection of over 30 promoters and 140 genes...characterize positive hits. This work has been published in Cell. The Moffat laboratory maintains a website...Carlson RCIscript-GoldyTALEN, pC-GoldyTALEN Tom Ellis pTAL5-BB, pTAL6-BB David Grunwald pCS2TAL3-DD,...Center has kindly constructed and deposited an unpublished set of Zika gene plasmids to Addgene’s repository... prototrophy, can also aid in designing self-establishing metabolically cooperating (SeMeCo) communities... -
Molecular Biology Protocol - Restriction Digest of Plasmid DNA
TypeProtocol...target a wide variety of recognition sequences. For a list of many commonly used restriction enzymes, visit... -
Protocol - How to Ligate Plasmid DNA
TypeProtocol...compatibly digested vector backbone. This is accomplished by covalently connecting the sugar backbone ... -
Immunocytochemistry
TypeProtocol...reproducibility and accelerates science. Here, we list the specific equipment, reagents, and methods that... -
Transfection for Recombinant Antibodies
TypeProtocol...reproducibility and accelerates science. Here, we list the specific equipment, reagents, and methods that... -
AAV Purification by Iodixanol Gradient Ultracentrifugation
TypeProtocol...Right image adapted by permission from Macmillan Publishers Ltd: Nature Protocols Grieger, Joshua C., Vivian... -
What is Polymerase Chain Reaction (PCR)
TypeProtocol...ends of the newly synthesized strands. Materials List Reagents for each 50µL PCR: Thin-walled PCR tube... -
Virus Protocol - Generating Stable Cell Lines
TypeProtocol...deliver antibiotic resistance. This protocol was established using 293T cells but can be adapted to alternative... -
Coomassie Purity Stain of Recombinant Antibodies
TypeProtocol...reproducibility and accelerates science. Here, we list the specific equipment, reagents, and methods that... -
Affinity Purification of Recombinant Antibodies with Protein A or Protein G
TypeProtocol...reproducibility and accelerates science. Here, we list the specific equipment, reagents, and methods that...