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Showing: 121 - 140 of 144 results

  1. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...pyogenes URA3 Wyrick pCRISPomyces-2 61737 Bacteria BbsI yes, cut S. pyogenes Apramycin Zhao pRB1017 59936...none S. pyogenes Zon pCRISPomyces-1 61736 Bacteria BbsI yes, cut S. pyogenes Apramycin Zhao pMB70 47943 Worm...activate, interfere, or nick. Selection , such as Puromycin or EGFP Cloning enzyme used for insertion of your...

  2. Microbiology Resources

    Type
    Collection
    ... Helicobacter pylori Listeria monocytogenes Mycobacterium sp. Neisseria sp. Pseudomonas sp. Ralstonia ...application and by color. External Resources European Saccharomyces Cerevisiae Archive for Functional Analysis (...Reagent Program (Link opens in a new window) Saccharomyces Genome Database (SGD) (Link opens in a new window...

  3. Tetracycline Inducible Expression

    Type
    Collection
    ...inducible expression of shRNA; neomycin selection; plasmid 21915 has puromycin selection TetR On Wiederschain...from pCW57.1. rtTA was replaced with tTA, and Puromycin was replaced with Blasticidin selection. Please...

  4. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...marker such as puromycin, you can (also) select for the positive colonies by puromycin selection. You ...transfected cells (for example, plasmid with a puromycin resistance cassette, such as PX459 ). After you...

  5. Botman-Teusink Yeast FP Collection

    Type
    Collection
    ...cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Yeast. 2004 Jun;21(8):661-70. doi...antibiotic selection markers for engineering in Saccharomyces cerevisiae. Microb Cell Fact. 2013 Oct 25;12...

  6. Control AAV Preps

    Type
    Collection
    ...dependent 8 Deisseroth 98927 pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 CAG GFPsm_myc (does not fluoresce) Cre ...

  7. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...Addgene plasmid ID 48139), which include GFP and puromycin as selectable markers, respectively, or constructs...consists of DMEM supplemented with 2% penicillin/ streptomycin and 1% L-glutamine, which is used for MEL cells...

  8. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Loren Looger AV-1-PV3661 98927-AAV1 pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 Control Loren Looger AV-10-PV0101...Boyden AV-1-PV3511 98926-AAV1 pAAV.CAG.GFPsm-myc.WPRE.SV40 Loren Looger AV-1-ALL856 100048-AAV1 pAAV.CAG.LSL.tdTomato...

  10. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...reporter vector expressing firefly luciferase with neomycin resistance. Other Resources Protocols Bloom lab...

  12. Retrovirus Plasmids

    Type
    Collection
    ... For cloning and gene expression; select with puromycin or screen for GFP Bartel 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE...

  13. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Port 49330 pAc-sgRNA-Cas9 dU6 BspQI Transfection Puromycin yes, cut Ji-Long Liu 45946 pU6-BbsI-chiRNA dU6...

  14. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...-Puro Mammalian, piggyBac hU6 pegRNA BsmBI No Puromycin Jacob Giehm Mikkelsen 173222 pPBT-PE2-PuroTK-pegRNA_GG...

  15. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...NGG) 42875 pCRISPR BsaI E. coli, S. pneumoniae Kanamycin none, need Cas9 plasmid Marraffini 42876 pCas9...

  16. CRISPR Plasmids - Plants

    Type
    Collection
    ... As Cpf1 Qi 91715 pKEE401 yes, cut S. pyogenes Neomycin Chen Do you have suggestions for other plasmids...

  17. Ras Pathway

    Type
    Collection
    ...protein, LST8 homolog MTOR Mechanistic target of rapamycin NF1 Neurofibromin 1 NFE2L2 Nuclear factor, erythroid...

  18. CRISPR References and Information

    Type
    Collection
    ...cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2...

  19. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TGGAAGCGGACGCGAGTCaagtcacctctggaagCCTGAACGATTCGCAACA mycn TAL3124 & TAL3125 TGCAGCCGTGTTTCTACCcggacgaggatgacttCTACTTCTGCAGACCAGA...
Showing: 121 - 140 of 144 results