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... efficient when the gRNA targets your gene of interest’s promoter, enhancer, or the beginning of the coding...

...you to create fusion proteins with your gene of interest. Most of these plasmids are available to industry...

...promoter or regulatory regions of your gene of interest. If the plasmid that you choose does not also ...

...specific promoter or enhancer for your gene of interest. These modifications persist over time and are...

...Category Empty Backbones Study your protein of interest by fusing it to a fluorescent protein. Michael...

...a component to find plasmids with your gene of interest. Browse Cancer Research Plasmids by Pathway mTOR...

... and place them in the public domain without restriction. Many SGC plasmids from the Toronto, Oxford and...

...tables, refer to Addgene's Optogenetics Guide . Interested in more news about optogenetics research? Find...

...simultaneously. By fusing sfGFP and/or mCherry to genes of interest, it is thus possible to study the effect of the...

... of the MCC community. They support educators interested in integrating MDH-related research into their...

... as pooled AAVs either directly to a region of interest or systemically using a capsid capable of crossing...

...genome are every 500 bp. TAL effectors have some restrictions (for example, the target must start with a T...

...containing the transgene or genetic elements of interest to introduce in cells. A packaging plasmid (or...

... novel AAV vector targets ON bipolar cells and restores visual function in a mouse model of complete congenital...

...used as empty backbones to express your gene of interest in plants. You can search the table for features...

...cell lines. Features include (i) Esp3I (BsmBI) restriction sites for full compatibility with the Golden ...

... or by RT-PCR. Isolate your PCR product from the rest of the PCR reaction using a kit, such as the (Link...assist with restriction enzyme digestion (usually 3-6bp). Restriction Site: Your chosen restriction site for...fusing the upstream restriction site to the forward primer and the downstream restriction site to the reverse...product is now ready for restriction digestion. Digest Your DNA Set up restriction digests for your PCR product... Plasmid Cloning by PCR You may also like... Restriction Digest of Plasmid DNA DNA Ligation Bacterial ...of a piece of DNA and at the same time adding restriction sites to the ends of that piece of DNA so that...that it can be easily cloned into a plasmid of interest. For this example, we will describe how to copy...

...reads from PCR amplicons that cover your locus of interest and correspond to edited and non-edited cell populations...

...method, mouse strain, target cell type or tissue of interest, and model organism under study. *Many engineered...

...very low or nonexistent. Scientists continue to wrestle with the possibility of postnatal and germline ...