...Filaments Utrophin (aa# 1-261) GFP William Bement 26740 mCherry-UtrCH Actin Filaments Utrophin mCherry ...cycle) Cep170 EGFP Erich Nigg 41163 pEGFP PICH Centromeres (dependent on cell cycle) PICH EGFP Erich Nigg...EGFP Ken-Ichi Takemaru 54234 mEmerald-PLK1-N-16 Centrosome Plk1 mEmerald* Michael Davidson 54491 mCherry-Lifeact...

...plasmids are in a lentiviral backbone, with some retroviral and AAV backbone options. Plasmids for constitutive...protocols Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors Toolbox Optimized shRNA design ... in a new window) . Wiznerowicz, M., Szulc, J., Trono, D. (2006). Tuning silence: conditional systems ...

...apamycin (mTOR) is a key metabolic regulator controlling cell growth and proliferation. mTOR, a serine...in cancer promotes the conditions needed for uncontrolled cell growth and proliferation, including an ...Kris Wood) References mTOR signaling in growth control and disease. Laplante M, Sabatini DM. Cell. 2012...

...CRISPR Read Addgene's CRISPR Guide for a general introduction to CRISPR technology. Addgene's worm CRISPR ...silencing in the germline. A "FLP-Out" system for controlled gene expression in Caenorhabditis elegans. - ...Hubbard Lab. Plasmids for the temporal and spatial control of gene expression in C. elegans by combining expression...

...the location and type of MECP2 mutation can be a strong predictor of symptom severity. Broadly, early gene... in a new window) RSRT iPSC lines and isogenic controls , patient-specific Infinity BiologiX - RSRT cell...refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology or the mammalian CRISPR...

...mind: CRISPR screening experiments require electroporation to amplify the pooled library and the use of...GBC) 85968 Barcode Human Weissman 3rd N/A N/A Retroviral Mouse Genome-wide CRISPR Knockout Library 104861...104861 Knockout Mouse Teichmann N/A (retroviral) 5 90,230 RNA-Binding Protein Pooled CRISPR Knockout Library...

...pHIV-Zsgreen - Mammalian Expression (this is a bicistronic vector rather than a fusion protein vector) ZsGreen1...- Mammalian Expression pMXs-mRFP1 - Mammalian Retroviral Expression mCherry 587 610 16 4.5 15 min Monomer...proteins Insect Sutherland Lab Plasmids - For multicistronic expression of GFP and/or mCherry, with neomycin...

...application-specific validation and consistent quality control measures. We work with trusted partners and experts... by sharing our protocols and quality control methods. Learn More Guide New to using antibodies...

... CRISPR Blog Posts CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in DNA instead...mutants can also be used with a repair template to introduce specific edits via homology-directed repair (HDR...

... Cut Fully functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location...frameshifts, creating loss of function alleles. To introduce specific genomic changes, researchers use ssDNA...

... These AAV encode tetracycline-inducible tools/controls and tetracycline transactivators that can be used...TEVp EF1a-driven, Cre-dependent Cre-dependent, bicistronic expression of designer pro-taCasp3 and TEVp for...

...100% efficiency. Unlike lentiviruses or other retroviruses , adenoviruses do not integrate into the host...pShuttle-CMV-F2A-T2A-Venus Shuttle For production of viruses with multicistronic expression + Venus tag; See plasmid 62622 for...

...undergo application-specific validation and quality control by Addgene as well as by our trusted partner labs...Acknowledgement (RSA). This form can be completed electronically by the scientist who will be using the materials...

...stress granules 114411 TNNI1-mEGFP AICSDP-34 mEGFP Troponin I, slow skeletal type Sarcomeric thin filaments...Nuclear speckles 159744 DMD-mEGFP AICSDP-55 mEGFP Dystrophin Costameres 159745 TFAM-mEGFP AICSDP-72 mEGFP ...

...fluorescent protein) in striatal Pvalb+ neurons. Note strong cortical Pvalb and L5 ET neuron expression. Jonathan...3xCore version of DLX enhancer). AAV vector for strong & rapid SYFP2 expression in forebrain GABAergic...

...construction of RAS pathway gene expression vectors for in vitro and in vivo research. The RAS Initiative - at the...colorectal cancer. Zenonos K, Kyprianou K. World J Gastrointest Oncol. 2013 May 15; 5(5): 97–101. doi: 10.4251...

...available and more are currently undergoing quality control analysis in our lab and will be made available ... Plasmid Description Industry PI Due to export control regulations, MERS and SARS-CoV materials are available...

...undergo application-specific validation and quality control by Addgene as well as by our trusted partner labs...

...undergo application-specific validation and quality control by Addgene as well as by our trusted partner labs...

...Biofuels Natural Product Pathways Efflux Pumps Flux Control Metabolism Plasmids Search the table by keyword...