We narrowed to 520 results for: pes

...cell types. A thorough understanding of the genotypic and phenotypic changes in the various types of cancer...incessant chemical activity, and consists of other cell types, especially immune cells and cells of epithelial...antitumor treatment strategies can be enhanced. Types of immunotherapeutics In lieu of the cancer-immune...use whole cell therapeutics consisting of various types of immune cells. Dendritic cells (DCs) are particularly...heterogeneity Tumors are composed of a wide variety of cell types. This cell heterogeneity is an important element...

...request the specific serotypes you need instead of receiving a kit with all the serotypes, since these 20 ...samples of EGFP-expressing AAV packaged in various serotypes. This collection is now complete! pAAV-CAG-GFP...expanding the inventory of these vectors to more serotypes. We currently package four variants of these biosensors...recently released several recombinases in various serotypes, and will continue to grow this collection. Of...

...identify and/or facilitate the evolution of new phenotypes, and forward genetics harnesses this at a large...grown in a turbidostat. Only mutate particular types of nucleotides. Both the Bassik Lab and the Chang... The Dueber Lab used EvolvR to discover novel genotypes that result in a spectinomycin resistant phenotype...These results not only uncovered novel resistance genotypes, but also provide a better understanding of the...system and the flexibility to address many different types of research questions. Are you ready to start EvolvR-ing...

...visualization of an enzymatic reaction. Types of ELISA There are four different types of ELISAs, which differ in the...and the detection antibody recognize different epitopes on the target protein, so they do not compete ...which multiple antibodies that recognize different epitopes are available; this type of ELISA is extremely...antibody available for your antigen as the other types of ELISAs require two different antibodies. As mentioned...

...handful of natural serotypes that can cross the BBB. However, these natural serotypes (AAV9, AAVrh10, and...sites can be used to prevent expression in cell types not of interest. Systemic capsids packaging Cre-...transgene expression to specific tissues and cell types. If desired, spatiotemporal control of transgene...cells, which is available in both AAV9 and AAV1 serotypes, allowing sequential administration (Chen et al... encompasses more molecularly-defined neuronal subtypes and with less bias among them than AAV-PHP.eB ...systemic AAVs for targeting distinct brain cell types. Nature Methods, 17(5), 541–550. https://doi.org...

... carry out genomic editing. Specifically, these types of CRISPR systems work using a multi-Cas complex.... Class 1 is further broken down into different types, with Cas3 systems making up type I, the most abundant... These type Is can then be further broken into subtypes of A–G, which come from different species and ...complexity. Figure 1: Schematic of Class 1 CRISPR types. Class 1 includes type I with a Cas3 effector, type...and type IV with an unknown effector. Type I has subtypes A through G, with different compositions of the...

...techniques that make tissues transparent are enabling microscopes to visualize the cellular architecture of whole...foundation for all subsequent versions of LSFM microscopes (3,4). After this initial application, Stelzer...microscopy vs. confocal microscopy In confocal microscopes, optical sectioning of a specimen is based on...photomultipliers used to detect light in confocal microscopes are less efficient than the modern cameras used...along the illumination path. The shadows appear as stripes in the acquired image (5).  A way to get around...

... under-informed with respect to how different serotypes, promoters, and injection methods are best suited...norepinephrine expressing neurons. We tested four serotypes containing an expression cassette with an ubiquitous... Fig. 1: Testing of three different AAV serotypes (AAV1, 8, 9) injected into the A2 noradrenergic...conditions (3,5,8), injection titers (1,2), and cell types used for packaging viral particles (6), can influence...vector performance in various brain regions and cell types. Power in numbers A Pubmed search for “AAV” and ...

...Advantages of ICE Determine Editing Efficiency and Types of Edits with Low-Cost Sanger Sequencing   ICE uses...and determine the profiles of all the different types of edits that are present and their relative abundances...ability to analyze and interpret data about these types of edits is critical to the CRISPR workflow. For...includes visual representations of all detected edit types in the sample. The visualization helps the researcher...sequencing data. Furthermore, ICE can analyze more types of editing experiments than other Sanger sequencing-based...

... Plans to develop tools to study their genotypes and phenotypes were presented in a series of quick (10...and effect hypotheses between gene function and phenotypes in diverse plants, animals, microbes, viruses...model for plant adaptation and speciation. Their phenotypes are diverse and they inhabit all habitats - even...plants share RNA, proteins, and DNA. Euprymna scolopes (Joshua Rosenthal) The Hawaiian bobtail squid ...

...includes small epitopes fused to each XFP. Secondary antibodies that target these epitopes and fluoresce...Brainbow, now exist for a variety of species, cell types, and research applications. Labeling neurons with...ubiquitous promoters, meaning that not all cell types can be labeled, and that while multiple copies of...systems have been developed for tracking various cell types and organisms and for improving the level of detail...

...available commercially, and most common buffers have recipes available online if you prefer the DIY method. ...re interested in. Table 1 lists the most common types of gels for SDS-PAGE running conditions and will...chemistry and/or specialty gel options. Table 1: Types of gels  Gel Type Protein sizes Running ... large, look for a larger pore size (0.45 µm).  Types of transfers Your transfer options are wet, semi-dry...read fairly soon after the reaction has started. Types of chemiluminescence enzymes The two most common...proteins, and their differing amounts of available epitopes for that particular antibody, this truly does ...

...primary antibodies are all of different species/isotypes and that each secondary has a different color ...Lab, using recombinant antibodies with different isotypes. (A) Neocortex labeled with anti-pan-Nav (magenta...using primary antibodies from different species or isotypes, so that the secondary antibodies don’t cross-... You could also consider strategies using other types of affinity reagents like fluorescently-labeled ... IF. Here, we’ll walk you through the necessary types of controls for a multiplex experiment.  Check for...

...to delete sequences and introduce mutations and epitopes with unprecedented ease. These new C. elegans ...(GFP; Tzur et al., 2013), or rescue of mutant phenotypes (unc-119) (Dickinson et al., 2013). These approaches... by isolating animals with particular visible phenotypes. These approaches may become even more powerful...30-60 basepair homology arms to knock in large epitopes, such as GFP (Paix et al., 2014). A further development...significantly enriches for knock-in of 2x and 3xFLAG epitopes into other, non-linked loci; pha-1(e2123) mutant...

...exhibit profound differences in tropism between cell types (astrocytes, microglia, neurons, etc.) and even ...interest. We recommend testing several different serotypes to determine which one is the best for your cells...Efficiency, Tropism and Axonal Transport of AAV Serotypes 1, 2, 5, 6, 8 and 9 in the Mouse Brain. PLoS ONE...Viral Vectors Pseudotyped with Viral Capsids from Serotypes 1, 2, and 5 Display Differential Efficiency and... WH, Young D (2021) AAV Targeting of Glial Cell Types in the Central and Peripheral Nervous System and...

... for further improvements. Types of teams There are, of course, many types of teams. Some teams may be... existing. Here are a few types of teams that exist at Addgene - team types that could exist anywhere ... cause ever since. Unlike the previous two team types, the skills are not cherry-picked. We take what ...

.... grouped the systems into three distinct types and subtypes using polythetic classification based on ...total of eight evolutionarily conserved CRISPR subtypes comprised of more than 40 cas gene families have...system and are not translatable to the other CRISPR subtypes. Still, the large and dynamic range of CRISPR ...

...antibodies bind different protein sequences called epitopes and scientists can use these to tag their proteins...of an antibody that recognize and bind specific epitopes (Ewert et al., 2004). This scFv hybrid provides... HA frankenbody is that it remains bound to HA epitopes for minutes in living cells, meaning that scientists... stitching technique could be applied to other epitopes and scaffolds to build a more robust set of frankenbodies...

...adeno-associated viral vectors (AAV) not all serotypes are equal; while some serotypes are typically high yielding, for...improve AAV yields. Although we only investigated 3 serotypes it’s possible that this approach will be beneficial...beneficial for a host of other AAV serotypes; it will be exciting to test this approach with different capsids...

...both types of HMO biosensors and displayed her findings at the Spring 2017 ACS meeting. She hopes these...advantage over other inducible enzyme technologies. She hopes that other researchers will be able to use similar.... In addition to her research endeavors, Fatima hopes to practice her science communication skills throughout...