...polyclonal antibodies contain a mix of different isotypes and subclasses. They each have their own advantages...secondary antibodies are broad and recognize multiple isotypes from a species, like a general anti-mouse antibody...antibody. Others recognize specific isotopes within that species, like an anti-mouse IgG antibody. Some ...polyclonal primary antibody contains a mix of different isotypes and subclasses, use a broadly reactive secondary... this post has explained when and why antibody isotypes matter and helps you choose the right antibody...from Addgene The Antibody Guide Antibodies 101: Isotypes Antibodies 101: Choosing the Right Antibody   ...

...isolate individual cells with unique spatiotemporal phenotypes from large heterogeneous cultures.  HeLa...SPOTlight was used to image over 3 million cell types expressing variants of Yellow Fluorescent Protein...new viral preps from recent months: Expanded serotypes are now available for Gordon Fishell Lab constructs...constructs targeting GABA neurons. Serotypes include AAV1, and AAV9. Find viral preps here pAAV-hSyn-Cre-P2A-dTomato...Deisseroth viral preps here Coming soon! New AAV serotypes from the Sanford Boye lab with enhanced retinal...retinal tropism. Find the upcoming serotypes here  ...

... cutting ninjas that deliver CRISPR to multiple types of targets: primary cells, embryos, and animals....animals. Nanoblades can also carry different types of CRISPR cargo including RNPs for indel formation via NHEJ...of mice, with the potential to be used for other types of CRISPR applications. Let’s take a look at the...BaEVRless) had high rates of transduction for most cell types they tested. Interested in using Nanoblades? Plasmids...efficiencies were between ~50-70% for these four cell types (Table 1). Additionally, hiPSCs and mouse BM cells...DNA-cutting ninjas which can stealthily delivery many types of CRISPR editing cargo to difficult to target cells...

... easy to buy, cultivate, and have interesting phenotypes to study. In addition, the Sánchez lab has made...plasmid deposit. These plasmids enable two main types of experiments: Whole mount in Situ Hybridization...tissue. This is useful for labeling specific cell types and for viewing how gene expression changes through...plasmids can result in  the labeling specific cell types or entire organs in planarians. For example, the...paste, and feed the mixture to the worms. The phenotypes of RNAi knockdown treatment should be apparent...apparent after three dsRNA feedings. Some of the phenotypes are not evident until after amputation. For example...

..., Vibrio cholerae, Xanthomonas campestris pv. campestris, X. campestris pv. malvacearum, Zymomonas mobilis...Shewanella spp., Thiobacillus spp., Xanthomonas campestris IncQ RSF1010, R300B, R1162 Gram-negative...salmonicida, Yersinia enterocolitica, Xanthomonas campestris, X. maltophilia IncW pSa, pR388 Gram-...Salmonella typhimurium, Vibrio cholerae, Xanthomonas campestris   In addition to incorporating broad host...

...cell types involved in neurodegenerative diseases, the Cole Lab selected promoters for cell types directly...understanding the function of cell types in the nervous system. While certain cell types, such as principal excitatory...neuronal subtypes was not available until now. In order to target specific neuronal subtypes such as inhibitory...its genes are poorly characterized. The Gross lab hopes these libraries will be used to advance our collective...

...cell types. A thorough understanding of the genotypic and phenotypic changes in the various types of cancer...incessant chemical activity, and consists of other cell types, especially immune cells and cells of epithelial...antitumor treatment strategies can be enhanced. Types of immunotherapeutics In lieu of the cancer-immune...use whole cell therapeutics consisting of various types of immune cells. Dendritic cells (DCs) are particularly...heterogeneity Tumors are composed of a wide variety of cell types. This cell heterogeneity is an important element...

...request the specific serotypes you need instead of receiving a kit with all the serotypes, since these 20 ...samples of EGFP-expressing AAV packaged in various serotypes. This collection is now complete! pAAV-CAG-GFP...expanding the inventory of these vectors to more serotypes. We currently package four variants of these biosensors...recently released several recombinases in various serotypes, and will continue to grow this collection. Of...

...identify and/or facilitate the evolution of new phenotypes, and forward genetics harnesses this at a large...grown in a turbidostat. Only mutate particular types of nucleotides. Both the Bassik Lab and the Chang... The Dueber Lab used EvolvR to discover novel genotypes that result in a spectinomycin resistant phenotype...These results not only uncovered novel resistance genotypes, but also provide a better understanding of the...system and the flexibility to address many different types of research questions. Are you ready to start EvolvR-ing...

...visualization of an enzymatic reaction. Types of ELISA There are four different types of ELISAs, which differ in the...and the detection antibody recognize different epitopes on the target protein, so they do not compete ...which multiple antibodies that recognize different epitopes are available; this type of ELISA is extremely...antibody available for your antigen as the other types of ELISAs require two different antibodies. As mentioned...

...handful of natural serotypes that can cross the BBB. However, these natural serotypes (AAV9, AAVrh10, and...sites can be used to prevent expression in cell types not of interest. Systemic capsids packaging Cre-...transgene expression to specific tissues and cell types. If desired, spatiotemporal control of transgene...cells, which is available in both AAV9 and AAV1 serotypes, allowing sequential administration (Chen et al... encompasses more molecularly-defined neuronal subtypes and with less bias among them than AAV-PHP.eB ...systemic AAVs for targeting distinct brain cell types. Nature Methods, 17(5), 541–550. https://doi.org...

... carry out genomic editing. Specifically, these types of CRISPR systems work using a multi-Cas complex.... Class 1 is further broken down into different types, with Cas3 systems making up type I, the most abundant... These type Is can then be further broken into subtypes of A–G, which come from different species and ...complexity. Figure 1: Schematic of Class 1 CRISPR types. Class 1 includes type I with a Cas3 effector, type...and type IV with an unknown effector. Type I has subtypes A through G, with different compositions of the...

...techniques that make tissues transparent are enabling microscopes to visualize the cellular architecture of whole...foundation for all subsequent versions of LSFM microscopes (3,4). After this initial application, Stelzer...microscopy vs. confocal microscopy In confocal microscopes, optical sectioning of a specimen is based on...photomultipliers used to detect light in confocal microscopes are less efficient than the modern cameras used...along the illumination path. The shadows appear as stripes in the acquired image (5).  A way to get around...

... under-informed with respect to how different serotypes, promoters, and injection methods are best suited...norepinephrine expressing neurons. We tested four serotypes containing an expression cassette with an ubiquitous... Fig. 1: Testing of three different AAV serotypes (AAV1, 8, 9) injected into the A2 noradrenergic...conditions (3,5,8), injection titers (1,2), and cell types used for packaging viral particles (6), can influence...vector performance in various brain regions and cell types. Power in numbers A Pubmed search for “AAV” and ...

... Plans to develop tools to study their genotypes and phenotypes were presented in a series of quick (10...and effect hypotheses between gene function and phenotypes in diverse plants, animals, microbes, viruses...model for plant adaptation and speciation. Their phenotypes are diverse and they inhabit all habitats - even...plants share RNA, proteins, and DNA. Euprymna scolopes (Joshua Rosenthal) The Hawaiian bobtail squid ...

...Advantages of ICE Determine Editing Efficiency and Types of Edits with Low-Cost Sanger Sequencing   ICE uses...and determine the profiles of all the different types of edits that are present and their relative abundances...ability to analyze and interpret data about these types of edits is critical to the CRISPR workflow. For...includes visual representations of all detected edit types in the sample. The visualization helps the researcher...sequencing data. Furthermore, ICE can analyze more types of editing experiments than other Sanger sequencing-based...

...includes small epitopes fused to each XFP. Secondary antibodies that target these epitopes and fluoresce...Brainbow, now exist for a variety of species, cell types, and research applications. Labeling neurons with...ubiquitous promoters, meaning that not all cell types can be labeled, and that while multiple copies of...systems have been developed for tracking various cell types and organisms and for improving the level of detail...

...available commercially, and most common buffers have recipes available online if you prefer the DIY method. ...re interested in. Table 1 lists the most common types of gels for SDS-PAGE running conditions and will...chemistry and/or specialty gel options. Table 1: Types of gels  Gel Type Protein sizes Running ... large, look for a larger pore size (0.45 µm).  Types of transfers Your transfer options are wet, semi-dry...read fairly soon after the reaction has started. Types of chemiluminescence enzymes The two most common...proteins, and their differing amounts of available epitopes for that particular antibody, this truly does ...

...primary antibodies are all of different species/isotypes and that each secondary has a different color ...Lab, using recombinant antibodies with different isotypes. (A) Neocortex labeled with anti-pan-Nav (magenta...using primary antibodies from different species or isotypes, so that the secondary antibodies don’t cross-... You could also consider strategies using other types of affinity reagents like fluorescently-labeled ... IF. Here, we’ll walk you through the necessary types of controls for a multiplex experiment.  Check for...

... for further improvements. Types of teams There are, of course, many types of teams. Some teams may be... existing. Here are a few types of teams that exist at Addgene - team types that could exist anywhere ... cause ever since. Unlike the previous two team types, the skills are not cherry-picked. We take what ...