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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 322 results for: phy

Showing: 121 - 140 of 322 results

  2. An Introduction to Adenovirus

    Type
    Blog Post
    ...In April 2022, physicians in Scotland began noticing unusual cases of acute hepatitis in the pediatric...culprit for these unusual cases was either 1) a physiological effect of SARS-CoV-2 toward destabilizing one...

  4. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...culture At Addgene we organically developed the philosophy of  “work hard, play hard.” Part of the reason...department) right in your breakroom. 5. Throw some physical activity into the mix It’s easy to sit around ...

  5. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...living cells, enabling the study of dynamic, physiological processes. Re-engineering GFP to increase its...GFP through mutagenesis in order to improve its physical and biochemical properties. In 1995, Roger Y. ...

  6. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...Syndrome was first described in 1966 by Austrian physician, Andreas Rett, who tried to raise awareness and...amount of protein to each brain cell to restore physiological levels. Much research is now focused on therapeutic...

  7. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...size. From the pool of shorter orthologs, only Staphylococcus aureus Cas9 (SaCas9, 1053 amino acids) displayed...Zhang F (2015) In vivo genome editing using Staphylococcus aureus Cas9. Nature 520:186–191 . https://doi.org...

  8. Hot Plasmids - August 2020

    Type
    Blog Post
    ...by one-step immobilized metal ion adsorption chromatography (IMAC) using Ni-NTA magnetic beads. Workflow...CRISPR Plasmids and Resources page.  PAC-MAN (prophylactic antiviral CRISPR in human cells) is a CRISPR-Cas13...

  9. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...started in 2005 in Kansas by Bobby Henderson, a physics graduate from Oregon State University. Find the...cutting-edge imaging technologies such as array tomography, super-resolution STORM imaging, and electron...

  10. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...immediate-early”) and consequently regulates neuronal physiology by promoting the development of neurons and synapses...contributions of transcript isoforms to neuronal physiology. The Day Lab’s neuron-optimized lentiviral vectors...

  11. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...1972) Protein a from Staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent...

  12. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...’ve turned the available into the accessible. Physical access Addgene is located in the USA, but our ...increasing the accessibility of our repository, from physical materials to useful information, but we’re not...

  13. How to Be an Excellent Trainee

    Type
    Blog Post
    ...to do? Walk through the protocol mentally (or physically where relevant) and identify any gaps or questions...bring them to your mentor. Pro tip! Consider physically walking through the protocol, by going to each...

  17. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...General Hospital where he is now an attending physician. He performs post-doctoral research in the lab...of the Belarusian State University Series of Physiological, Biochemical and Molecular Biology Sciences....

  18. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...Matlashov et al., 2020) derived from bacterial phytochrome (BphPs) photoreceptors have higher apparent cellular...fluorescent proteins engineered from bacterial phytochromes. Curr Opin Chem Biol 27, 52-63 (2015). Shcherbakova...

  19. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...components of the cytoskeleton. They provide cells with physical structure, separate chromosomes during mitosis...RhoGEF is a great way to stimulate and study these physical processes. Joachim Goedhart and colleagues in ...

  20. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...for planthopper and cereal genomic studies. New Phytol 223:2120–2133 . https://doi.org/10.1111/nph.15889... Editing and Transcriptional Regulation. Plant Physiol 169:971–985 . https://doi.org/10.1104/pp.15.00636...
Showing: 121 - 140 of 322 results