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We narrowed to 327 results for: phy

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Showing: 121 - 140 of 327 results

  1. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...culture At Addgene we organically developed the philosophy of  “work hard, play hard.” Part of the reason...department) right in your breakroom. 5. Throw some physical activity into the mix It’s easy to sit around ...

  2. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...living cells, enabling the study of dynamic, physiological processes. Re-engineering GFP to increase its...GFP through mutagenesis in order to improve its physical and biochemical properties. In 1995, Roger Y. ...

  3. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...started in 2005 in Kansas by Bobby Henderson, a physics graduate from Oregon State University. Find the...cutting-edge imaging technologies such as array tomography, super-resolution STORM imaging, and electron...

  4. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...’ve turned the available into the accessible. Physical access Addgene is located in the USA, but our ...increasing the accessibility of our repository, from physical materials to useful information, but we’re not...

  5. How to Be an Excellent Trainee

    Type
    Blog Post
    ...to do? Walk through the protocol mentally (or physically where relevant) and identify any gaps or questions...bring them to your mentor. Pro tip! Consider physically walking through the protocol, by going to each...

  6. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...1972) Protein a from Staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent...

  7. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...immediate-early”) and consequently regulates neuronal physiology by promoting the development of neurons and synapses...contributions of transcript isoforms to neuronal physiology. The Day Lab’s neuron-optimized lentiviral vectors...

  8. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...Syndrome was first described in 1966 by Austrian physician, Andreas Rett, who tried to raise awareness and...amount of protein to each brain cell to restore physiological levels. Much research is now focused on therapeutic...

  9. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...size. From the pool of shorter orthologs, only Staphylococcus aureus Cas9 (SaCas9, 1053 amino acids) displayed...Zhang F (2015) In vivo genome editing using Staphylococcus aureus Cas9. Nature 520:186–191 . https://doi.org...

  10. Hot Plasmids - August 2020

    Type
    Blog Post
    ...by one-step immobilized metal ion adsorption chromatography (IMAC) using Ni-NTA magnetic beads. Workflow...CRISPR Plasmids and Resources page.  PAC-MAN (prophylactic antiviral CRISPR in human cells) is a CRISPR-Cas13...

  14. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...components of the cytoskeleton. They provide cells with physical structure, separate chromosomes during mitosis...RhoGEF is a great way to stimulate and study these physical processes. Joachim Goedhart and colleagues in ...

  15. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...Matlashov et al., 2020) derived from bacterial phytochrome (BphPs) photoreceptors have higher apparent cellular...fluorescent proteins engineered from bacterial phytochromes. Curr Opin Chem Biol 27, 52-63 (2015). Shcherbakova...

  16. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...General Hospital where he is now an attending physician. He performs post-doctoral research in the lab...of the Belarusian State University Series of Physiological, Biochemical and Molecular Biology Sciences....

  17. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...for planthopper and cereal genomic studies. New Phytol 223:2120–2133 . https://doi.org/10.1111/nph.15889... Editing and Transcriptional Regulation. Plant Physiol 169:971–985 . https://doi.org/10.1104/pp.15.00636...

  18. Evolution of Lab Techniques

    Type
    Blog Post
    ...labeling it. The label can then be visualized by autoradiography and X-ray film for radioactively labeled probes...fragments of a DNA sequence of interest to create a physical map of the DNA. Starting in the 1970s, restriction...

  19. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...binds to endogenously expressed protein at physiologically relevant levels. Some vendors will also test...tissues that are known to express the protein at physiologically relevant levels (not overexpression systems...

  20. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ... refractory to enzymatic digestion, however, a physical fragmentation strategy is preferable. Nebulization...tank with a pressure regulator. Other methods for physical shearing exist as well, such as sonication and...
Showing: 121 - 140 of 327 results