Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 134 results for: plasmid transfection

Showing: 121 - 134 of 134 results

  1. Immunocytochemistry

    Type
    Protocol
    ... expresses the protein, such as cells transfected with a plasmid to express the protein of interest, and...Immunocytochemistry You may also like... Antibody Plasmid Collection Antibody Blog Posts Antibody Guide Western...

  2. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...NeuroMab/Trimmer Lab Plasmid Collection R-mAb Plasmids scFv Plasmids Nanobody Plasmids Protocols References...following plasmids can be used to produce R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target...More scFv Plasmid Collection The following plasmids can be used to produce scFvs from transfected mammalian...Nanobody Plasmid Collection The following plasmids can be used to produce nanobodies from transfected bacterial... Antibody Plasmid Collection NeuroMab Plasmid Collection...Protocols for the generation R-mAbs plasmids, production of R-mAbs in transfected cells and their validation can...generate R-mAbs by transiently transfecting the plasmids into mammalian tissue culture cells (e.g., HEK293, ...

  3. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...different plasmid. In order to produce rAAV vectors, three plasmids are needed: Transfer plasmid (also known...the packaging plasmid. For a summary of all AAV plasmid components, see the AAV Plasmid Elements table... three production plasmids (transfer, packaging, and helper plasmid) are transfected into cells which ...as cis plasmid) — containing the transgene of interest between the two ITRs. Packaging plasmid (also known...chosen plasmid is compatible with cloning methods available in your lab. When cloning your plasmids, be ...possibility that recombination between the transfected plasmids could lead to the production of replication-competent...all of Addgene’s transfer plasmids are replication deficient. These plasmids can be used to create viral...

  4. Tetracycline Inducible Expression

    Type
    Collection
    ...Mammalian shRNA Plasmids Collection Cre-lox Plasmids Collection Stem Cell Research Plasmids Collection Tetracycline.... ID Plasmid Description Transactivator Promoter PI 21915 Tet-pLKO-puro Lentiviral Tet-On plasmid for ...with puromycin selection. See Plasmid #85973 for blasticidin and Plasmid #85972 for hygromycin selection...search our collection for all dox-regulated plasmids . ID Plasmid Description PI 63800 PB-TRE-dCas9-VPR Tet-inducible...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...expression and find plasmids for your experiments using tet- or dox-inducible systems. Plasmid... Plasmid Collections Tetracycline...

  5. Adenovirus Guide

    Type
    Guide
    ...you generally need two plasmids: Shuttle plasmid (also known as transfer plasmid) — containing the transgene...shuttle plasmid. For a summary of all adenoviral plasmid components, see the Adenoviral Plasmid Elements...for recombination with the adenoviral plasmid. Adenoviral plasmid — containing the rest of the adenoviral...that recombination between the transfected adenoviral transfer plasmid and the E1 region integrated in...chosen plasmid is compatible with cloning methods available in your lab. When cloning your plasmids, be ...consists of two plasmids that eventually recombine to form one: Shuttle/transfer plasmid (e.g. pAdTrack...backbone plasmid containing the adenoviral genes necessary for virus production. The shuttle plasmid and the...

  6. Lentiviral Vector Guide

    Type
    Guide
    ...second-generation transfer plasmids as well. Third-generation plasmids include (Figure 4): Transfer plasmid — contains...Packaging plasmid 1 — contains gag and pol Packaging plasmid 2 — contains rev Envelope plasmid — contains...systems Packaging Plasmid One plasmid encoding gag , pol , tat , and rev Two plasmids, one encoding gag...lentiviral plasmids (three or four) are transfected into HEK293T cells using your preferred transfection method...Lentiviral Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose Transfer plasmid LTR ...separated into distinct plasmids for safety. For a summary of all lentiviral plasmid components, see the ...four, for third generation) plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest ...

  7. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Gamma-Retroviral Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose Transfer plasmid LTR ... of all gamma-retroviral plasmid components, see the Gamma-Retroviral Plasmid Elements table . In order...gamma-retroviral vectors, you need three plasmids (Figure 2): Transfer plasmid — contains transgene, sgRNA, or ...capacity Packaging plasmid — contains packaging genes gag and pol Envelope plasmid — contains packaging...chosen plasmid is compatible with cloning methods available in your lab. When cloning your plasmids, be ... The three plasmids described above (envelope, packaging, and transfer) are co-transfected into the HEK293T...on separate plasmids ( in trans ). This method, therefore, reduces the number of plasmids that are required...

  9. Western Blot

    Type
    Protocol
    ...Blot Western Blot You may also like... Antibody Plasmid Collection Antibody Blog Posts Antibody Guide Immunocytochemistry...you know expresses the protein, such as cells transfected with the protein of interest, and a negative ...

  10. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...Guide Pages Find CRISPR Plasmids for Your Experiments Deposit Your New Plasmid Tools with Us  ...binding sites. This PITCh plasmic is cotransfected with a plasmid carrying Cas9, the PITCh-gRNA, and the...Integration into Target Chromosomes). Using their PITCh plasmids, GFP knock-in cell lines can be created in about... knock-ins, some labs have used NHEJ for whole plasmid integration; however, since NHEJ is error-prone...cloning. An overview of PITCh. The PITCh plasmid contains a GFP-Puro cassette flanked by 5' and .... The PITCh-gRNA creates two DSBs in the PITCh plasmid to release the cassette, and an insertion-locus-specific...insertion. PITCh can also be adapted for whole-plasmid integration if you’d like to integrate a larger...

  11. Antibody Guide

    Type
    Collection
    ... specific hybridoma cells into plasmids. Plasmids are then transfected into cells and induced to express...little variation. Recombinant plasmids - Antibodies can be produced from plasmids, with an antibody’s genetic...immune cells, or through the use of recombinant plasmids. Animals - Antibodies produced in animals make...genetic code inserted into the plasmid. This produces the least amount of variation between antibodies, allows...should produce the same antibody clone, or from plasmid-based expression. Monoclonal antibodies are necessary... ready-to-use recombinant antibodies made from plasmids in our collection. View Antibodies...

  12. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...amazing plasmid technologies developed by our community of depositors. With over 40,000 plasmids avaliable...amazing new plasmid tools that have come through our doors in 2015. Do you have a favorite new plasmid tool ...system plasmids are available through Addgene and are already very popular. Use these plasmids to increase... can visualize: Cell Structure Plasmids: Cell Structure Plasmids: ER mCherry-Sec61 beta BFP-Sec61...luciferase reporter gene plasmid pGL3 pGL3 luciferase reporter gene plasmids have been used extensively...find all of our Hot Plasmid articles on our website. We update the our Hot Plasmids articles every quarter...Read Our CRISPR 101 Posts Read Our Plasmids 101 Posts Read All of Plasmid Technology Posts Resources at ...

  13. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    ...23953120. PubMed Central PMCID: PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan...2015): 1334-1341. PubMed PMID: 26258682. Find plasmids from this publication at Addgene. 3. Kirchhofer... in their 293 cell culture system. In cells cotransfected with GFP and the GBPs, GFP linked the two GBPs...

  14. Antibody Guide

    Type
    Guide
    ... specific hybridoma cells into plasmids. Plasmids are then transfected into cells and induced to express...little variation. Recombinant plasmids - Antibodies can be produced from plasmids, with an antibody’s genetic...immune cells, or through the use of recombinant plasmids. Animals - Antibodies produced in animals make...genetic code inserted into the plasmid. This produces the least amount of variation between antibodies, allows...should produce the same antibody clone, or from plasmid-based expression. Monoclonal antibodies are necessary... ready-to-use recombinant antibodies made from plasmids in our collection. View Antibodies...
Showing: 121 - 134 of 134 results