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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 356 results for: plat

Showing: 121 - 140 of 356 results

  1. TALEN Guide

    Type
    Collection
    ...bind adenenine with lower specificity). With the template for studying this type of technology already laid...

  2. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ... connect the imaging community and to create a platform for discussions and potential collaborations. ...loads of opportunities to create communities, platforms, and to be a part of projects that connect people...realize. Being at a university provides the perfect platform for practicing initiative and for training in ...

  3. Bioinformatics at Addgene

    Type
    Blog Post
    ...help us work in large formats, such as 96-well microplates, and thanks to our dedicated product and development...pipeline. After reviewing and testing several platforms for engineering pipelines, we selected Nextflow...workflow would handle plasmid sequences on a 96-well plate basis, but for rapid testing and refinements, we...

  4. Hot Plasmids - November 2023

    Type
    Blog Post
    ...PE6b was very efficient for short, unstructured templates and significantly reduced undesired edits (indels...complex edits, such as long or highly structured templates. Cas9 variants PE6e–g often had even higher editing...potential to reduce negative side effects. This platform offers a promising approach for experimental therapies...

  5. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    .../L yeast extract, and 5 g/L NaCl) or on LB-agar plates containing glucose and L-arabinose at 37oC for ... arabinose and colonies on arabinose-containing plates will be visibly white, and gas vesicles in cells...from left to right: colony whiteness on inducer plates, white inclusions in cells with GVs via phase contrast...

  6. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...general, technical replicates can be thought of as "plate controls". They are NOT independent and are typically...transfection of multiple separate wells (within the same plate) with purified plasmid from the same aliquot/preparation... the same source and were incubated on the same plate at the same time. Although the replicate control...

  7. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...phenotype. Incubating the phage display library with plate-bound antigen helps select for high affinity scFvs...bacteriophages. This library is incubating with plate-bound antigen to select for scFvs with high affinity...antibodies  Read about another reagent sharing platform for antibodies, the Developmental Studies Hybridoma...

  8. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ... into DNA. This also requires an exogenous DNA template. HDR, however, is a very low-efficiency process... is >30nt from the proximal ends of the repair template (Yang et al., 2013). This means that, for gene...the Director of R&D in the Genetic Perturbation Platform at the Broad Institute and has worked with many...

  9. Viral Production

    Type
    Collection
    ...isolating genomic DNA. This DNA is then used as a template to amplify a portion of the intended lentiviral...

  10. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...repaired by homologous recombination with a repair template.  Each zinc finger recognizes a nucleotide triplet...advertisement of the technology on various non-scientific platforms, CRISPR has also transcended into the world of... and reverse transcriptase. Using an elongated template + guide RNA called pegRNA (prime editing RNA),...

  11. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...charged as they pass through a metal deflection plate. Each droplet contains a single cell that is assessed...for a desired parameter is collected in a tube or plate, while a droplet containing a negative cell is discarded.... Many FACS sorters are compatible with 96-well plates as collection vessels and can sort single cells...

  12. COVID-19 Resources

    Type
    Collection
    ...coronaviruses. Addgene assisted this effort by providing a platform for the distribution of plasmid-based tools that...

  17. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...directed repair templates (see the supplementary info from the paper for details on repair template design). ... Methods 2012. Mar 11;9(5):493-8. MXS cloning platform for imaging and flow cytometry applications Designing...relatively easy to design constructs and repair templates to insert TAP tag fusions directly into the genome...

  19. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...months. Ampicillin breaks down especially fast and plates should be used witin 1 month for optimal efficiency... them to media hotter than about 55oC and store plates/stocks wrapped in foil if a light-sensitive antibiotic...

  20. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...creatively to deliver a highly specific, programmable platform well-suited for various innovative biological ...enabling access to multiple plasmids used for various platform applications. For more information on CRISPR/Cas...
Showing: 121 - 140 of 356 results