...Nickase mutants can also be used with a repair template to introduce specific edits via homology-directed...

...Esposito Lab for use with the Gateway® Cloning Platform (Life Technologies™) to permit construction of...

... Special Collections EXPRESs Plasmids Human Platelet EXPRESs Plasmids for Human Erythrocyte Receptors...

...possibly resulting in frameshifts. If a repair template with high homology to the DNA surrounding the ...

...most commonly used is the Illumina NGS platform. This is the platform used by Addgene’s sequencing partner...of DNA, or templates, are spatially separated from each other. These attached DNA templates are then amplified... again producing ~1,000 copies of each template. Each template is then replicated using the modified bases...antibiotic(s) to add to your LB media or LB agar plates. Antibiotic Recommended Stock Concentration Recommended...the 4 nucleotides, a DNA polymerase enzyme, the template DNA to be copied, and a primer. A primer is a ...is a high-throughput, multi parallel sequencing platform that can generate sequencing data for up to 600...normal” bases in the reaction. All the sequenced templates are then aligned to each other to assemble the...

...phenotype. Incubating the phage display library with plate-bound antigen helps select for high affinity scFvs...bacteriophages. This library is incubating with plate-bound antigen to select for scFvs with high affinity...antibodies  Read about another reagent sharing platform for antibodies, the Developmental Studies Hybridoma...

... into DNA. This also requires an exogenous DNA template. HDR, however, is a very low-efficiency process... is >30nt from the proximal ends of the repair template (Yang et al., 2013). This means that, for gene...the Director of R&D in the Genetic Perturbation Platform at the Broad Institute and has worked with many...

...repaired by homologous recombination with a repair template.  Each zinc finger recognizes a nucleotide triplet...advertisement of the technology on various non-scientific platforms, CRISPR has also transcended into the world of... and reverse transcriptase. Using an elongated template + guide RNA called pegRNA (prime editing RNA),...

...charged as they pass through a metal deflection plate. Each droplet contains a single cell that is assessed...for a desired parameter is collected in a tube or plate, while a droplet containing a negative cell is discarded.... Many FACS sorters are compatible with 96-well plates as collection vessels and can sort single cells...

...Jaramillo Lab Riboregulation Keasling Lab BglBrick Platform , Terpenoid Production , and Propionate Induction...

... to utilize HDR for gene editing, a DNA repair template containing the desired sequence must be delivered...the gRNA(s) and Cas9 or Cas9 nickase. The repair template must contain the desired edit as well as additional...arms. Depending on the application, the repair template may be a single-stranded oligonucleotide, double-stranded...double-stranded DNA plasmid. When designing the repair template, do not include the PAM sequence present in the...the genomic DNA. This step prevents the repair template from being a suitable target for Cas9 cleavage..., you could alter the PAM sequence in your HDR template with a silent mutation that does not change the...this feature, a Cas13a-based molecular detection platform, termed SHERLOCK, has been used to differentiate...

...set of plasmids for building homologous repair templates that incorporate a self-excising drug selection...

...bind adenenine with lower specificity). With the template for studying this type of technology already laid...

...isolating genomic DNA. This DNA is then used as a template to amplify a portion of the intended lentiviral...

... Enzyme-linked immunosorbent assay (ELISA) is a plate-based assay that uses standard curves to quantify...Overview of application Coat antigens to the well of a plate, either by charge interactions (direct) or by a ...several wash steps. Add primary antibody to the plate. After incubation, wash to remove unbound antibody...Activate the conjugate and read the output via a plate reader. Special considerations The two most common...of interest is attached to the well of a 96-well plate. A conjugated primary antibody is used to bind to...be sorted (separated and deposited into tubes or plate wells) by fluorescence activated cell sorting (FACS...

...Associate Director of the Genetic Perturbation Platform at the Broad Institute, John Doench. A genetic...Associate Director of the Genetic Perturbation Platform at the Broad. His mom always told him that he ...

...asked the individuals behind some of these new platforms what their motivations were for developing these...research in their field including datasets and platforms for discussing new developments, and more. As ...

...high-volume hybridoma sequencing on the NeuroMabSeq platform. Mitchell et al. Sci Rep. 2023 Sep 27. See More...

...by an inexpensive and widely available imaging platform: ultrasound. Challenges of in vivo imaging with...ultrasound imaging. Ultrasound is an attractive imaging platform for in vivo imaging experiments because  ultrasound...

...system with your homologous recombination (HR) template, you could then: Confirm your HR by doing Restriction...screen for positive ones.  -- OR -- If your HR template has a selection marker such as puromycin, you ...