Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 992 results for: ran

Showing: 121 - 140 of 992 results

  1. TALEN Plasmids and Kits

    Type
    Collection
    ...in vitro transcription as an alternative. Based on transient double-selection of transfected cells, the...new tools for genome engineering. Fusions of transcription activator-like (TAL) effectors of plant pathogenic... RVD domains designed for rapid TALEN assembly. Kiran Musunuru and Chad Cowan Ekker Lab TALEN Kit All ...strong nuclear localisation tags and repress transcription initiation when targeted to DNA sequences within...Gate-assembled TALEN, both in microinjected embryos and transfected cells. TALEN expression is driven by the strong...reporter vector pGL4-SSA can be used for single-strand annealing assay, which enables the evaluation of...destination vectors can be used to create TALE-transcription factors and are used at the final assembly step...

  2. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ...Browse the Zhang lab’s comprehensive human transcription factor collection, available at Addgene. The...The collection can be used for screening transcription factors for cellular programming or cancer drug ...Library The MORF library is a comprehensive human transcription factor collection that contains 1,836 genes ...collection, can be purchased at Addgene. Each transcription factor isoform has a unique 24 bp barcode that...that simplifies identification of the transcription factor isoform in pooled screens. This collection ...collection and library can be used for screening transcription factors for applications such as cellular programming...cancer drug resistance. Protocols & References A transcription factor atlas of directed differentiation. Joung...

  3. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...(see paper) Transfection none, need Cas9 plasmid Calarco 47943 pMB70 cU6 BsaI Transfection none, need ...Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...functional CRISPR/Cas enzymes will introduce a double-strand break (DSB) at a specific location based on a gRNA-defined... cut Goldstein 42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid Joung 46170 PU6::klp... plasmid Boxem 47941 pMB60 T7 BsaI In vitro transcription none, need Cas9 plasmid Boxem 47912 SP6-sgRNA-scaffold...sgRNA-scaffold SP6 AflII In vitro transcription none, need Cas9 plasmid Sternberg 177783 L4440_BioBrick-...

  4. Addgene Packaged on Request: Scope of Service

    Type
    Collection
    ...reasons: Transfer plasmids with sequence from the 5′ to the 3′ ITR exceeding 4.7 kb Transfer plasmids ... Terms of Purchase and the applicable material transfer agreements (MTAs) and limited use label licenses...through our Packaged on Request service, Addgene guarantees a titer of at least 4 x 10 12 GC/mL for the AAV1... you know that we don’t expect to produce our guaranteed titer, and will give you the option to proceed...proceed with your order, or not, without a guaranteed titer. Any titer expectations communicated by email ...email will supersede the general guarantees above. We will have our expert viral vector production team carefully...our viral production does not yield the titer guaranteed here or by email, Addgene will contact you to...

  5. Genetic Code Expansion

    Type
    Collection
    ...protein translation machinery. To expand the genetic code, 4 major changes to the standard translation machinery...Bacterial Ahmed Badran 209739 pAB228i24 G1 PylRS Methanogenic archaeon Bacterial Ahmed Badran 209740 pAB228q12...jannaschii Bacterial Ahmed Badran 209741 pAB228r3 Mlum1RS Bacterial Ahmed Badran 209742 pAB228p PylRS M. ...mazei Bacterial Ahmed Badran 209743 pAB228p4 PylRS M. mazei Bacterial Ahmed Badran 209744 pAB228v TrpRS...cerevisiae Bacterial Ahmed Badran 209745 pAB228v6 TrpRS S. cerevisiae Bacterial Ahmed Badran 209746 pAMC070n1a12...acids. Expanding the Genetic Code In protein translation, an aminoacyl-tRNA synthetase (aaRS) loads its...Barrick 122650 Mm-PylRS-AF/Pyl-tRNACUA PylRS M. mazei trans-cyclooct- 2-ene-lysine (TCOK) Mammalian TAG Howard...

  6. p53 Pathway

    Type
    Collection
    ... bind transcriptional co-activators and modulate transcription. The list of p53 transcriptional targets...collection of plasmids for the p53 pathway. p53 is a transcription factor and tumor suppressor activated by cellular...p53 Gene List References Background p53 is a transcription factor and tumor suppressor activated by cellular...mutations interfere with p53’s ability to activate transcription, and they also have a dominant negative effect... receptor superfamily, member 10b E2F-1 E2F transcription factor 1 Fas Fas cell surface death receptor... Tyner SD, Venkatachalam S, Choi J, Jones S, Ghebranious N, Igelmann H, Lu X, Soron G, Cooper B, Brayton... p53 mutations in human cancers. Hollstein M, Sidransky D, Vogelstein B, Harris CC. Science. 1991 Jul ...

  7. Typing CRISPR Systems

    Type
    Blog Post
    ...6105. https://doi.org/10.1093/nar/gku241 Cong, L., Ran, F. A., Cox, D., Lin, S., Barretto, R., Habib, N..... Type I systems are also employed as CRISPR transposases, by leaving out Cas3. Type I is further subdivided...complex (blue subunits) with a guide RNA (green and orange) bound to the target DNA with Cas3 (pink). Complex...promoter, allowing for easy multiplexing. The double-stranded breaks caused by Cas12 create short 3’ overhangs...paradoxically called V-K, is a CRISPR-associated transposase (CAST) that can insert large fragments of DNA...use a Cas14 enzyme, and uniquely target single-stranded DNA non-specifically. Type VI Type VI is defined...interference, as it can target and degrade select RNA transcripts, or be engineered to conduct direct RNA editing...

  8. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...10.1146/annurev-biophys-062215-010822 Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I.... NUC HNH Cut DNA target strand RuvC Cut DNA non-target strand CTD (or PI) Recognize PAM...information on these regions.   Cas9’s transition from weak, random binding to precision gene editing requires...left) and transparent (right). The RNA guide sequence is extended as a single unpaired strand in the center...10 of the guide RNA, prearranged for base pairing with a complementary DNA strand. These 10 nucleotides...the non-target strand for the PAM sequence and doesn’t mind if the PAM complementary strand is mismatched...of the PAM in the non-target DNA strand (dark gray). Target DNA strand, light gray.   Once it has found...

  9. Viral Production

    Type
    Collection
    ...of Pennsylvania Vector Core. Transfections are performed using the transfer plasmid, a plasmid encoding...cell cultures are inspected for sterility. Transducibility Some viral vectors are tested in vitro and ...section for images). Figure 2: AAV Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634...filtration through a 0.45 µm polyethersulfone (PES) membrane. The preparations are then aliquoted, frozen, ...opens in a new window) ). Briefly, 293T cells are transduced with serial dilutions of a lentiviral vector,... taken off-line and discarded. Confirmation of Transfer Plasmid Addgene uses a rigorous barcode matching...integrated provirus using PCR. This assay involves transducing cells with serial dilutions of the lentiviral...

  10. Bacterial Expression Systems

    Type
    Collection
    ...periplasmic space between the inner and outer membranes. Targeting proteins to the periplasm reduces bacterial... Protein Interactions Förster resonance energy transfer (FRET) and bimolecular fluorescence complementation...that when a donor fluorophore is excited, it can transfer its energy to an acceptor fluorophore if they ...allows you to fuse proteins of interest to transcriptional regulatory elements that will turn on gene ...selection of plasmids using tetracycline-regulated transcriptional activation or repression. The BIOFAB plasmid...that element. When this activation requires a transcription factor to bind to a small molecule, the reporter...DNA-binding specificity of proteins such as transcription factors. Addgene Blog Luciferase Technologies...

  11. Resolute Plasmid Collection

    Type
    Collection
    ...CRISPR knockout library targeting human transmembrane transporters. Feedback Please send your feedback to...carrier proteins (SLCs) are the largest family of transporters in the human genome with approximately 450 identified... Medicines Initiative 2 Joint Undertaking under grant agreement No 777372. This Joint Undertaking receives...allow a fast and efficient sub-cloning into a wide range of Gateway-compatible pDEST vectors. ID Plasmid ...overexpression library of human SLC proteins. Human Transporter Knockout Libraries 213695 221409 221410 Superti-Furga...

  12. Brain Initiative Collection

    Type
    Collection
    ...identified by depositors and/or when BRAIN Initiative grants are noted in publications associated with Addgene...-GfaABC1D-mCherry-hPMCA2w/b Expresses a plasma membrane calcium pump hPMCA2w/b Baljit Khakh 112000-AAV1...flip-excision switch, for AAV production or direct transfection, slow variant Kaspar Podgorski 135421-AAV1 pAAV-syn-FLEX-axon-jYCaMP1s...flip-excision switch, for AAV production or direct transfection, slow variant Kaspar Podgorski 135424-AAV1 pAAV-syn-jYCaMP1s...Synapsin1 promoter, for AAV production or direct transfection, slow variant Kaspar Podgorski 135630-AAV1 pAAV-S5E2...interneurons. For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent... expressed under the mammalian promoter (EF1a) Francois St-Pierre 179460-AAV1 pAAV-EF1a-DIO-JEDI-2P-WPRE...

  13. Antibody Production

    Type
    Collection
    ...extracted from cells, separated by SDS-PAGE, transferred to a membrane, and labeled with the antibody and a species-specific...expression vectors, human embryonic kidney cells are transfected with the plasmid(s) encoding the heavy and light...anti-GFP antibody), the target antigen is first transiently expressed in mammalian cells. To learn which ... The fluorescence pattern is compared to an untransfected control. Immunohistochemistry (IHC) Tissue sections...antibody. Protein expression is compared to an untransfected control and the protein size is confirmed. Residual...contain low levels of co-purified plasmid from transfection. This residual plasmid is extracted and amplified...

  14. New England Biolabs Cell-Imaging Plasmid Collection

    Type
    Collection
    ...with a recognizable sub-cellular localization (membrane, nucleus, etc.) are also available Non-toxic to... is based on the human O6-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein, and is suitable...Vector SNAP-tag Empty backbone for stable and transient mammalian expression 101133 pSNAP-CaaX Control...Control Plasmid SNAP-tag Control for endomembrane (CaaX box) localization 101129 pSNAPf-Cox8A Control Plasmid...Vector CLIP-tag Empty backbone for stable and transient mammalian expression 101134 pCLIPf-H2B Control...pMCP-tag (m) Vector ACP/MCP-tag Empty backbone for transient mammalian expression 101126 pACP-tag (m)- 2 Vector...Vector ACP/MCP-tag Empty backbone for stable and transient mammalian expression 101132 pMCP-GPI Control Plasmid...

  15. AAV Packaged on Request

    Type
    Collection
    ...tools that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments, both...of the applicable transfer plasmid. We will help you gain legal usage of the transfer plasmid from the ...service lets scientists choose from over 4,000 transfer plasmids to be packaged in their choice of seven...chemogenetic, optogenetic, recombinase, and biosensor transfer plasmids in our repository are eligible for this...several important steps: MTA facilitation for the transfer plasmid, including legal approval and an implementation...

  16. Deisseroth INTRSECT Collection

    Type
    Collection
    ...including dual-transgenic recombinase-expressing mouse lines 2–9 and combinations of transgenic recombinase-expressing... Cre AND Flp, here expressed through a double transgenic animal strategy (PV-2a-Cre; SOM-IRES-Flp). Image...in a new window) of Flp-recombinase-expressing transgenic mouse and rat lines. The Fenno Lab maintains ... Rayport S. 2018. Dopamine neuron glutamate cotransmission evokes a delayed excitation in lateral dorsal...Neurons in Barrel Cortex Reveals Local and Long-Range Circuit Motifs. Cell Rep. 28(13):3450-3461. PubMed...Krook-Magnuson E. 2019. A novel population of long-range inhibitory neurons. Preprint (Link opens in a new...PubMed (Link opens in a new window) Mandelbaum G, Taranda J, Haynes TM, Hochbaum DR, Huang KW, Hyun M, Umadevi...

  17. Caltech Systemic Capsids

    Type
    Collection
    ....AX with GRE or transgenic animal Oligodendrocytes - AAV-PHP.eB with GRE or transgenic animal Brain vascular....AX with GRE or transgenic animal L5 & Inhibitory - AAV-PHP.eB with GRE or transgenic animal L2/3 & L4...AAV.CAP-B10 with GRE or transgenic animal Excitatory - AAV.PHP.N with GRE or transgenic animal For PNS applications... pAAV-S5E2-GFP-fGFP E2 regulatory element GFP, membrane-targeted GFP Control Dimidschstein 192552 pAAV-CAG... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

  18. Zebrafish Plasmid Collection

    Type
    Collection
    ... (~3 months), ease of care and breeding, and transparency of the embryo and larvae, it is easy to culture...standard toolbox of genetic methods, such as Tol2 transposons, Cre-mediated site-specific recombination, morpholinos...emerged as a model system for human disease and translational research, including the study of cancer, inflammation...-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference (CRISPRi...zebrafish - Julian Lewis Lab PhOTO Zebrafish: A Transgenic Resource for In Vivo Lineage Tracing during Development...examples of popular tools to use in zebrafish, ranging from optogenetics to tools for studying diseases...vectors designed to streamline the production of transgenic zebrafish for studying neurodegenerative disorders...

  19. COVID-19 Resources

    Type
    Collection
    ...envelope protein, E; nucleocapsid protein, N; and membrane protein, M), including plasmids for protein production...Anderson et al., 2020). BSG plasmids - (CD147), transmembrane glycoprotein of the immunoglobulin superfamily...proteolysis of SARS-CoV Spike protein, activating its membrane fusion function (Bosch et al., 2008). TACE plasmids... protease (Mpro) activity reporters Reverse transcriptase, protease, and polymerase constructs for SARS-CoV...Llamas Required - Synthetic Nanobodies Against Membrane Proteins Return to top Pooled Libraries Pooled... SARS-CoV-2 infection to host cells . Signal transduction and targeted therapy, 5 (1), 283. https://doi.org...

  20. CRISPR References and Information

    Type
    Collection
    ...sequence and checks for off-target binding. Supports a range of Cas enzymes (Cas9 and Cas12) and organisms. Developed...gene inactivation, NHEJ, and HDR. Includes a wide range of reference genomes, including animals, plants,...and cytosine (CBE) base editors. Includes a wide range of reference genomes, including animals, plants,...Ui-Tei Lab . CRISPick (Link opens in a new window) Ranks and picks candidate CRISPRko/a/i sgRNA sequences...for off-target binding. Currently supports a wide range of organims. Developed by the Michael Boutros Lab...and Wente Zebrafish: gRNA cloning, in vitro transcription, injection gRNA core ; Cas9 ; optimized Cas9...105 KB Jaenisch CRISPR plasmid summary for transcriptional activation dCas9 activators sgRNA dual expression...
Showing: 121 - 140 of 992 results