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We narrowed to 533 results for: try

Showing: 121 - 140 of 533 results

  1. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...GATC) Preparing unmethylated DNA, important when trying to cut with certain restriction enzymes (ex: ClaI...CCWGG) Preparing unmethylated DNA, important when trying to cut with certain restriction enzymes that are...

  2. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ...for career skills. Download a summary PDF here or try out the online Researcher Development Framework Planner...lab that has multiple collaborations, even with Industry labs Work on a big project that relies on a division...inviting others (be on a speaker committee) Follow industry publications like FierceBiotech to “learn the ...

  4. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...freezer. No more issues deciphering handwriting or trying to find to where the binder was moved. You’ll have...and you can’t remember where you put your flow cytometry protocol. Or maybe you can’t find the molecular...

  5. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...growth after overnight incubation. What went wrong? Try growing the culture for more time. Some bacterial...following into a 500 mL glass bottle: 4 g NaCl 4 g Tryptone 2 g Yeast Extract and dH 2 O to 400 mL Note: If...

  6. Validated gRNA Sequences

    Type
    Collection
    ...GAGTCCGAGCAGAAGAAGAA 47508 cut S. pyogenes 23792628 Joung ETC2, TRY, and CPC A. thaliana 71288 cut S. pyogenes 26193878...

  7. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...secondary antibody may be binding non-specifically – try optimizing your blocking and washing conditions or...are always new and improved variations you could try. We hope this post helped you start planning for ...referred to as immunocytochemistry (ICC) when the sample is cultured cells or immunohistochemistry (IHC) with....3001161 McLaughlin, K. (2019). Multiplexing Immunohistochemistry. Materials and Methods, 9. https://doi.org...antibodies available at Addgene Protocols for Immunocytochemistry Protocols for other antibody applications...

  8. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...unpublished evidence shows otherwise. Others will go on to try to apply these findings in their own work ultimately...likely that we’ll find a fluke when we’re simply trying to get our experiments to work, but part of good... sciences and featured speakers from academia, industry, nonprofits, and publishing. The livestream video...publishing community views reproducibility, how industry relies on reproducibility, and how you can push...

  9. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...or two hanging out around the lab and especially try to attend a lab meeting. Observe how the lab members...scientific meetings (and present)? Making the decision Try to rotate or interview in more than one lab when ...(topic and/or technical aspects) applicable to industry if this path interests you  (e.g. human disease...drug/device development? Does the advisor have industry connections such as serving on Boards? Does the...the publication record show evidence of industry collaboration? If you are considering a career at a small...

  10. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...one you use, of course, depends on what you are trying to do – there’s no “best” tool among them. While...best” gRNA depends an awful lot on what you are trying to do: gene knockout, a specific base edit, or ...Rees HA, Liu DR (2018) Base editing: precision chemistry on the genome and transcriptome of living cells...

  11. Antibodies 101: Validation

    Type
    Blog Post
    ...sensitivity, which can be problematic if you are trying to show that your antibody can distinguish between...Additional resources on the Addgene blog:  Still trying to choose an antibody? Check out our Selecting ...This is a good option for applications like immunohistochemistry on human tissues, when (1) other strategies...correspond to protein levels, so you perform immunohistochemistry with your antibody on these tissues (B)....recognized by your antibody. You perform immunocytochemistry on these mutant cells as well as a non-mutant...epitopes of this protein.   You perform flow cytometry using both antibodies, as well as a third unrelated...sample using the antibody and then uses mass spectrometry to identify the captured proteins. If the antibody...

  13. Science as Art

    Type
    Blog Post
    ...in an Open Source Lab Resources on Addgene.org Try the Plasmid Land Game ...

  17. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ...with high marker expression level. Labs have been trying different approaches to improve CTC capture, but... is small, highly stable and has this amazing chemistry locking the domain together - no one knows what.... Many other Gram-positive bacteria have this chemistry in their surface proteins. It is best studied ...

  18. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...fewer and fewer people use plastic bags.  We also try to recycle our discards according to the Cambridge...plastic materials are fairly inexpensive in this country and we are quick to throw out chipped or imperfect...
Showing: 121 - 140 of 533 results