Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 322 results for: virus

Showing: 121 - 140 of 322 results

  1. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    ...full-fledged pandemic. Containing the spread of this virus has become an utmost priority for countries around...method has been optimized to detect the SARS-CoV-2 virus directly using saliva without having to perform ... not give a positive result for highly similar viruses. Unlike Cas12a, Cas13a does not require strict ... methods.   Rapid detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR based DETECTR assay...in the saliva (Lucia et al., 2020). Detecting coronavirus using CRISPR-based SHERLOCK (Specific ​H​igh ​...ultrasensitive and visual detection of novel coronavirus SARS-CoV-2 Sensitivity: 4.6-11 copies/microliter...2020). An ultrasensitive, rapid, and portable coronavirus SARS-CoV-2 sequence detection method based on...

  3. AAV Q&A with Tim Miles

    Type
    Blog Post
    ... transfection efficiency when delivering these viruses via retro-orbital vs. tail vein systemic injections...Additional Resources on the Addgene Blog Using virus in your research - a primer for beginners Adenoviral...user Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page Subscribe to Viral Vectors...

  4. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    ...mammal called alpaca could neutralize the SARS-CoV-2 virus. But while the early results were promising, the...projects to a halt. None of us works directly with viruses or transmissible diseases, so we were left with...origins trace back to March 2020, when the first coronavirus lockdown forced us to put our research projects...

  5. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...familiar, ready-to-use adeno associated virus (AAV) and lentivirus are now available from Addgene. While...repository, you can instead have Addgene make the virus for you. Neuroscientists are particularly interested...

  6. AAV Viral Preps

    Type
    Collection
    ...Ready-to-use adeno-associated virus (AAV) available from Addgene's viral vectory packaging service. Browse...Service AAV Browse In-Stock AAV Adeno-associated virus (AAV) vectors are safe and useful gene delivery ...Ablation, CRISPR Monosynaptic Neuronal Tracing Rabies Virus-Based Serotype Serotype Testing Samples Small samples...

  7. COVID-19 Resources

    Type
    Collection
    ...Background The Coronavirus Disease-2019 (COVID-19) pandemic is caused by a novel virus strain from the... elucidate as much as they can about this novel virus. It is now known that SARS-CoV-2 entry, like that...suitable for generating SARS-CoV-2 spike pseudotyped virus. It also lists several luciferase and fluorescent... Spike protein constructs for making VSV pseudo virus Mammalian constructs Due to the size and complexity...mediating the entry of SARS-CoV and SARS-CoV-2 viruses. ( 1 ) TMPRSS2 - a serine protease that primes ...primes the SARS-CoV-2 S protein and is involved in virus entry into cells. ( 2 ) FURIN - an enzyme that cleaves... to the SARS-CoV-2 S protein and is involved in virus entry into cells. ( 4 ) Cathepsin L - a cysteine...

  8. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...catalog items. Virus-associated DNA Each viral vector service request will also include virus-associated ... the original plasmid that was used to make the virus. In order for Addgene to provide viral vector services... libraries. Viral preparation options include lentivirus and AAV. Viral...Order a high-quality and ready-to-ship AAV or lentivirus prep for your next experiment today! Browse AAV...

  9. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ...now important for accelerating research into this virus. One such example is cell lines. The American Type...Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-...Infected with Severe Acute Respiratory Syndrome Coronavirus. JVI 81:813–821 . https://doi.org/10.1128/jvi...

  10. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...this is likely to shear the envelope of your virus. o. Virus may be stored at 4°C for a few days, but should...decrease the efficiency of the virus, so Addgene recommends that you use the virus immediately or aliquot the...0.05-1 mL virus (add 0.5 mL for a high MOI, and 0.1 mL for a low MOI). Scale the amount of virus added depending....g. BL2+) suitable for handling HIV-derivative viruses. For transient knockdown of protein expression,...protocol can be scaled to produce different amounts of virus as needed. Day 1: a. For each plasmid to be transfected...infection time to between 4 – 20 hours. Remove the virus-containing media and replace with fresh media. Do...DS et. al. Cell . 2003. 115:199-208. PubMed . Lentivirus-delivered stable gene silencing by RNAi in primary...

  11. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...Santana et al., 2020) and plants viruses (barley yellow striate mosaic virus (Gao et al., 2019) or sonchus... the ubiquitously expressing cauliflower mosaic virus 35S promoter (CaMV 35S) or ubiquitin promoters (...sonchus yellow net rhabdovirus (Ma et al., 2020) have already been shown to be efficient ways to deliver CRISPR-Cas9...Z, Wang Y, Wang X (2019) Rescue of a plant cytorhabdovirus as versatile expression platforms for planthopper...

  12. Addgene: The First Twenty Years

    Type
    Blog Post
    ...have a resource like Addgene to all get the same virus.” — Grad student visiting Addgene’s booth at the...Addgene pivoted to support researchers combatting the virus. Our COVID-19 collection, comprised of 1302 plasmids...

  13. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...for improvement of optogenetic adeno-associated virus (AAV) vectors. Listed below are a few additional...Sabatini BL. 2012. Novel recombinant adeno-associated viruses for Cre activated and inactivated transgene expression...

  14. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...neural pathways was 8.5 x 10e10 GC/mL. The lot of viruses used (100798-AAV, lot #v15287, titer 1.7x10e13 ... (2018) An Alternate Route for Adeno-associated Virus (AAV) Entry Independent of AAV Receptor. J Virol...Monosynaptic Tracing Success Depends Critically on Helper Virus Concentrations. Front Synaptic Neurosci 12. https...

  15. Open Enzyme Collection

    Type
    Collection
    ...165510 pOpen-DNA Topoisomerase 1B Vaccinia Virus Vaccinia Virus DNA Topoisomerase 1B 165515 pOpen-EcoRIM...165546 pOpen-MMLV_RT (mut H) Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated... pOpen-MMLV_RT (lack H) Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH...

  17. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...Bogner, et al. 2015. Capsid Mutated Adeno-Associated Virus Delivered to the Anterior Chamber Results in Efficient...rAAV2tYF-CB-hRS1, a Recombinant Adeno-Associated Virus Vector Expressing Retinoschisin. Hum Gene Ther Clin...Transduction of Retina by Recombinant Adeno-Associated Virus Vectors. J Virol . Mar 28;90(8):4215-4231. PMID:...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Return...

  19. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ...proteins are a key component of lentiviruses. These proteins coat the virus and help determine the types ... of 2017 is the lentivirus envelope plasmid pMD2.G from Didier Trono’s lab! Lentivirus envelope vector... producing our ready-to-use lentivirus preps. You can find our lentivirus production protocol on our website...titering your lentivirus in another blog post. If you need a quick primer on how lentiviruses work, check...widely used and requested because it enables lentiviruses to infect many different types of cells. This...check out our lentivirus guide page. For more protocols and information, be sure to check out the Trono Lab...Additional Resources Quick Guide to All Things Lentivirus 5 Tips for Troubleshooting Viral Transductions...

  20. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ... enable the virus to enter host cells. To do this, they injected the spike protein from two different ...spike proteins in cell culture, and inhibit these viruses.  But llama immunization has its drawbacks: it...bind to coronavirus spike proteins (Wrapp et al., 2020), which sit on the surface of coronaviruses and enable...different coronaviruses into a llama over several days and isolated special camelid antibodies that bind to the...Structural Basis for Potent Neutralization of Betacoronaviruses by Single-Domain Camelid Antibodies. Cell ... carriers collection Find other COVID-19 and Coronavirus Plasmids & Resources ...
Showing: 121 - 140 of 322 results