We narrowed to 819 results for: cell
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TypeCollection...R-mAbs plasmids, production of R-mAbs in transfected cells and their validation can be found below. With these...transfecting the plasmids into mammalian tissue culture cells (e.g., HEK293, COS, CHO), from which the R-mAbs ...level expression we recommend using the HEK-2936E cell line engineered by Durocher et al., 2002 (Link opens...used to produce R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target Target Species Host Species...Pink1 Human M. musculus (mouse) IgG2b 227033 SynCAM1 cell adhesion molecule (Mus musculus) recombinant mouse...used to produce scFvs from transfected mammalian cells. Addgene ID Plasmid Hybridoma Target Target Species...to produce nanobodies from transfected bacterial cells. Addgene ID Plasmid Hybridoma Target Target Species...
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Magnetic Control of Proteins: More than a Dream
TypeBlog Post...visible whether expressed in E. coli, in mammalian cells, or in purified form, and didn’t need any added ... -
Droplet Digital PCR for AAV Quantitation
TypeBlog Post...used in the detection of rare sequences and single cell analysis. It is also being used in the detection... -
Transferable Skills Guide: Career Planning Resources
TypeBlog Post...attend. Societies, such as the American Society for Cell Biology (ASCB) or the American Chemical Society ... -
Career Insights: Technical Support Specialist
TypeBlog Post...issue was with a custom-made reagent to manipulate cells (manufactured by one of the larger biotech companies...Specifically, roles in technical support make excellent use of the skills developed by life science PhDs...about novel developments in the fields of gene and cellular therapies. Additional Resources on the Addgene... -
Deciphering the Mysteries of Behavior with Viral Vectors
TypeBlog Post...the mesocorticolimbic system in addiction. Front Cell Neurosci. 2014;8:466. PubMed PMID: 25653591. PubMed... -
Light Sheet Fluorescence Microscopy
TypeBlog Post... Weber, M. Mickoleit, J. Huisken. (2014) Methods Cell Biol.123, 193-215. Pubmed. W. Spalteholz. (1914)...transparent are enabling microscopes to visualize the cellular architecture of whole tissues in 3D with unprecedented... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...Cas protein and a target-specific gRNA in the same cell at the same time. Single plasmids containing both...Alternatively, gRNA-only plasmids can be used in cells or organisms in which Cas9 has been integrated into... -
QC Sequencing Technologies at Addgene
TypeBlog Post...named) “nanopores” that are attached to the flow cell membrane and have an electrical current running ... -
Antibodies 101: Normalization and Loading Controls for Western Blots
TypeBlog Post...Most housekeeping proteins are highly expressed in cells. This means that at sample concentrations appropriate...