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We narrowed to 616 results for: GAN

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Showing: 211 - 240 of 616 results

  1. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...celebrate! As we enter this new growth phase of the organization and the expansion of our impact, I’d like to...service and contribution! Leadership On an organizational level, we keep passing our audits with flying...even via Twitter. We love working with local organizations like Just-a-Start, the Northeastern co-op program...

  3. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ...CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page . Find a ...

  6. CRISPR in the Clinic

    Type
    Blog Post
    ...functional correction of the gene in clonally expanded organoids. This work, and many others, pushed the field ... post was originally written in 2014 by Kendall Morgan and updated in 2023 by Susanna Stroik.  References...of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients." Cell Stem Cell. ...

  7. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...concentrations and transients in living cells and organisms. Unlike chemical indicators, these DNA constructs...constructs can be delivered to intact tissues and organs noninvasively and target a defined population of cells...Genetically encoded fluorescent indicators for organellar calcium imaging. Biophysical journal. 2016 Sep...

  8. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...tech transfer team will communicate with your organization’s technology transfer office or other authorized.... rerio (zebrafish), D. melanogaster (fly), C. elegans (nematode), S. cerevisiae (budding yeast), S. pombe.... The name of the Principal Investigator and Organization where your plasmids were first created. From...

  15. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...of NHEJ, HDR, and MMEJ repair varies from organism to organism. MMEJ doesn’t yield a “perfect repair” like...researchers can exploit for CRISPR gene editing. In organisms where HDR is downregulated, MMEJ represents another...

  17. Plasmid Collections

    Type
    Collection
    ...Collections A modular set of plasmids from a depositor organized by a common use. Click here to view all collections...

  18. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...CRISPR functions along with plasmids categorized by organism by visiting our CRISPR plasmids page . Find a ...

  19. TALEN Engineering

    Type
    Collection
    ...yielded TALENs which function efficiently in: C. elegans ( Wood et al., Science 2011 ) Zebrafish ( Sander...

  20. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...Chromatography columns containing appropriate immobilized ligands are incubated with cell culture supernatant containing...antibodies. The antibodies bind the immobilized ligands and can be eluted once the other materials have...incubated with the cell suspension. Antibodies bind ligands on the beads and a magnet is used to separate the...

  21. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...slices, which are easier to expand than, say, a C. elegans (Damstra, et al. 2022; Yu, et al. 2020). With any...//doi.org/10.7554/elife.73775  Dani A, Huang B, Bergan J, et al (2010) Superresolution Imaging of Chemical...Wassie AT, et al (2020) Expansion microscopy of C. elegans. Elife 9:e46249. https://doi.org/10.7554/elife.46249...

  22. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...generated using random genomic fragments from the organism of interest, or a defined cDNA library (e.g. the...number of two-hybrid systems available in model organisms, including bacteria, alternative fungi (C. albicans...Enzymol. 1983;100:293-308. PubMed PMID: 6312261. 2. Keegan, L., Gill, G., and Ptashne, M. Separation of DNA...

  23. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

    Type
    Blog Post
    ...partnerships with like-minded individuals and organizations, like the Allen Institute, to achieve greater...together. The Allen Institute is a research organization that is committed to understanding some of the...reliable tools to help carry out that research. Organizations like the Allen Institute are committed to just...

  24. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...(CNS) orchestrates complex processes enabling organisms to control their movements and behavior. These...to study connectomics (how neural circuits are organized and how neurons connect to each other). Combined.... 2. Shepherd, G.M. (2004). In "The synaptic organization of the brain". Oxford University Press, New ...

  25. Lab to Office Culture Shock

    Type
    Blog Post
    ... number of career paths--industry, nonprofit organization, law office, journal editor, etc.--so everyone...around the world, such as BosLab here in Boston. Organizations like this one are always looking for scientists... own independent research!). There are also organizations like Science from Scientists, which try to bring...

  26. How to Write a Scientific Review Article

    Type
    Blog Post
    ...one either. Choose the topic and outline the organization of the review Once you start reading, there ...references) and it’s better to keep your references organized from the beginning. I also recommend using the...Manuscripts: Literature Searching, Reading, & Organizing Tips for Improving Your Next Manuscript 6 Tips...

  27. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ... that the drug prevents the growth of a living organism. As for multi-drug resistance: definitions vary...vary in the literature, but generally organisms with multi-drug resistance are resistant to three or more...is an intense amount of competition between microorganisms for available resources. This competition drives...

  28. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...over-express coding sequences from a given cell type or organism, have been used in a similar manner to Cas9 activators...lines (HEK293T, MCF7, U2-OS, Hela, N2A, 3T3) and organisms (Chavez et al., 2016). To learn more about the...Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden...

  29. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...distinct subcellular environments, including the organelles that compose the secretory pathway, endocytic...formation, and proteolytic cleavage. FPs localized to organelles are equally susceptible to these non-native modifications... Lee Snapp. "Fluorescent proteins in cellular organelles: serious pitfalls and some solutions." DNA and...

  30. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...non-human primates [7, 8], zebrafish [9], and c. elegans [10]. If used properly, optogenetics is sure to...Cellular and Molecular Biology from the University of Michigan and he is currently a Postdoctoral Fellow in the...neural activity in freely moving Caenorhabditis elegans." Nature methods 8.2 (2011): 147-152. PubMed PMID...
Showing: 211 - 240 of 616 results