We narrowed to 273 results for: aav
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TypeBlog Post...it easier to package for therapeutic delivery by AAV, which has strict capacity limitations. New efforts...Science, 362(6416): 839-842 (2018). 10.1126/science.aav4294 Ozcan, Ahsen, Krajeski, Rohan, Ioannidi, Eleonara...
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Fluorescent Protein Guide: Empty Backbones
TypeCollection...mGreenLantern - Mammalian Expression pAAV-CAG-mGreenLantern - Mammalian AAV Expression pBAD-mGreenLantern -...Crimson 611 646 29 4.5 24 min Tetramer pAAV-CAG-E2-Crimson - Mammalian AAV Expression pEB2-E2-Crimson - Bacterial...pcDNA-miRFP670nano3 - Mammalian Expression pAAV-hSYN-miRFP670nano3-WPRE-hGHpA - Mammalian AAV Expression pBAD/His-miRFP670nano3...pmiRFP680-N1 - Mammalian Expression pAAV-CAG-miRFP680 - Mammalian AAV Expression pDRF1 ymiRFP680 - Yeast...Expression miRFP713 690 713 7 3.5 Monomer pAAV-CAG-miRFP713 - Mammalian AAV Expression pmiRFP713-N1 - Bacterial...pmiRFP720-N1 - Mammalian Expression pAAV-CAG-miRFP720 - Mammalian AAV Expression Return to top Long Stokes... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...plasmids were developed to replace helper viruses in AAV production systems. We’re just skimming the surface... -
Plasmids 101: Terminators and PolyA signals
TypeBlog Post...effects of various terminators in the context of AAV transgene expression levels. Transcription Termination... -
Adenovirus Guide
TypeGuide...for replication. For more information about AAVs, read our AAV guide . Recombinant Adenoviral Vectors Wild-type...double-stranded DNA genome. Adeno-associated viruses (AAV), however, are small single-stranded DNA viruses ...viruses that belong to the Parvoviridae family. AAVs were discovered as a contaminant of adenovirus preparations...how they got their name, but they are not related. AAVs require the presence of adenoviral genes E1, E4,...Vectors Although recombinant adeno-associated viral (rAAV) vectors are often the preferred choice among researchers...expression cassettes are often too big to be packaged in rAAV vectors. This is the case of some commonly used ...first-generation rAdV vectors) surpasses that of rAAV vectors (~4.7 kb) and is further increased in second... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post...acids) means they can be delivered in vivo using AAV vectors. Having a way to shut off CRISPR-Cas systems... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog Post...AdVs have already a packaging capacity larger than AAV vectors. This capacity is large enough to carry the... -
PCR Applications for Copy Number Variation Assays
TypeBlog Post...Resources on the Addgene blog Droplet Digital PCR for AAV QuantificationPolymerase Chain Reaction Overview ... -
Viral Vectors 101: Chemogenetics
TypeBlog Post...targeting expression involve tissue specific promoters, AAV serotypes, and the ever-growing variety of recombinase...applications. Science 6436: eaav5282. https://science.sciencemag.org/content/364/6436/eaav5282.long Redfern CH, ... -
5 Tips for Troubleshooting Viral Transductions
TypeBlog Post...to their transduction has been shown to increase AAV transduction efficiency by approximately 15-fold ... -
CRISPR Plasmids - RNA Editing
TypeCollection...capabilities but are small enough to be packaged in AAV particles. Figure 1: Overview of RNA targeting with... -
Depositor Collections
TypeCollection...Protein Collection Allen Institute for Brain Science AAV Enhancer Collection Allen Institute for Cell Science... -
Simplify Cloning with in vivo Assembly
TypeBlog Post...amplify! Examples of this are the GC-rich ITRs of AAV genomic DNA encoding plasmids, or promoters such ... -
Hot Plasmids: Winter 2025
TypeBlog Post...Lee et al., 2024. The authors have deposited AAV plasmids for Cre-dependent and -independent versions...for the first time (Figure 3). The team also used AAV9 to deliver ASAP5 to the cortex and imaged voltage... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog Post...Plasmids Read Our Lentiviral Vector Guide Read Our AAV Guide ... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog Post...low-capacity vectors, such as adeno-associated viral (AAV) vectors. In 2017, Zetsche et al. developed a Cpf1... -
Light Sheet Fluorescence Microscopy
TypeBlog Post...Thy1-YFP-H mice). However, we recently developed AAV (adeno-associated virus)-based fluorescent labeling...cord and cranial nerve afferents labelled with a AAV8 expressing eGFP under the human ubiquitin C promoter... -
Plasmid Collections
TypeCollection...Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids Curated... -
Viral Vectors 101: Pseudotyping
TypeBlog Post...glycoprotein along with the TVA receptor in neurons using AAV and then delivering rabies dG pseudotyped with EnvA... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog Post...FLEX-vectors Learn More about Cre-Lox Check out Retrograde AAVs for Neurscience Research Resources on Addgene.org... -
CRISPR Activation: A Practical Guide
TypeBlog Post... CRISPR/Cas9 Learn How CRISPR Has Been Used with AAV Resources on Addgene.org Find Cas9 Activators Find... -
Lentivirus ddPCR Titration
TypeProtocol...Titration Assay Lentivirus Fluorescence Titration Assay AAV Titration by qPCR Addgene Viral Service Lentivirus... -
Mammalian RNAi Tools
TypeCollection... a lentiviral backbone, with some retroviral and AAV backbone options. Plasmids for constitutive expression... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog Post... allows for their delivery by viral vectors like AAV, which could be useful for delivering scFvs that ... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post... either by electroporation of plasmids, or using AAV vectors. The Imai lab also developed a version of... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog Post...occasions, shRNAs are transiently expressed using either AAV backbones or species-specific expression plasmids... -
Science Guides
TypeGuide...research, including: Lentivirus Adeno-associated Virus (AAV) Adenovirus γ-Retrovirus Read More... -
Adenovirus Plasmids
TypeCollection...Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook Adenoviruses are... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post...Acids Delivery, or GONAD (Gurumurty et al., 2016). AAV can also be used as a vector to deliver CRISPR/Cas9... -
Distribution to Industry
TypeCollection...Carrier Proteins (SLCs) from the RESOLUTE collection AAV Packaging from Addgene Parkinson's Research from ...