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We narrowed to 395 results for: AGA

Showing: 281 - 320 of 395 results

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The Materials Science of Optogenetics Experiments

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Blog Post

...opportunity to “reset” and become receptive to light once again. Therefore, light must be delivered in pulses. An...
Twenty Years of Addgene Sharing: CRISPR

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Blog Post

...most popular with our requestors. However, we can again see quick adaptation and steady use of all types...
PCR Applications for Copy Number Variation Assays

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Blog Post

...price point, which should be carefully balanced against the limitations of the assay. Fig....
Polymerase Chain Reaction Overview and Applications

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Blog Post

...PCR product is often visualized on a diagnostic agarose gel to confirm product presence, size, and relative...
Plasmids 101: Protein Expression

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Blog Post

...transformed into a bacterial cell. Transformed cells propagate, are induced to produce your protein of interest...
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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...University in Canada. He's happy to be contributing again to science. Acknowledgements Chan Zuckerberg ...
Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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Blog Post

...colony phenotype upon growth on LB/Cam/X-gal/IPTG agar plates, indicating successful reconstruction of ...
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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Blog Post

...Next Experiment Learn More About Designing gRNAs Against Your Favorite CREs Here Additional Resources on...
Addgene's Cancer Collection Pages Connect the Research Community

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Blog Post

... right places. Checks and balances: protecting against cancerous cells Luckily, there are several safety...
Google Forums Round Up: First Impressions of NgAgo

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... and development before it can truly be pitted against CRISPR head-to-head. Exciting features of NgAgo...
Plasmids 101: 5 factors to help you choose the right cloning method

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... to prevent any non-recombinant vectors from propagating. The ligation-independent methods like Gibson...
10 Basic tips for mammalian cell culture

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... Cell Culture Cloning Mammalian Cells with the Agarose Method Plasmids 101: Mammalian Vectors Resources...
Viral Vectors 101: Voltage Indicators

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Blog Post

...action potentials which are electrical charges propagated through multiple cells to do things like translate...
AAV Vector Quality Control: Going the Extra Mile with NGS

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...to the contaminant. Each contig is then BLASTed against the NCBI nucleotide collection database, and it...
Tips for Using BLAST to Verify Plasmids

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...result – blastx checks all six possible frames against the database. Another benefit is that a frameshift...
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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Blog Post

...other words, these parts can be used over and over again in combination with novel genes to get very precise...
Great Results Start with Great Standard Curves

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Blog Post

...concentration of a serially diluted standard is plotted against its OD560 measurements and the trendline y = 0.6561x...
An Introduction to Adenovirus

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Blog Post

... Vectors Overcome Preexisting Humoral Immunity against Human Adenoviruses In Vivo. J Virol, 73(8), 6930...
Viral Vectors 101: The Retroviral Lifecycle

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Blog Post

...emerges. Retrovirus life cycle Retroviruses go against the grain of the Central Dogma of Biology (gDNA...
SciComm: Taking Science to Elementary and Middle Schools

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Blog Post

...jobs, and an enthusiasm for coming back and trying again next year. There are many reasons why you might ...
Simplify Cloning with in vivo Assembly

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Blog Post

...vitro, before transforming it into E. coli for propagation. However, while not widely known, plasmid assembly...
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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Blog Post

...with 10 sgRNAs in the sgRNA metrics, confirming again that sgRNA design plays a significant role in library...
Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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Blog Post

...localization of BiFC constructs should also be compared against those of their endogenous counterparts (by immunocytochemistry...
A Guide to Starting Your Own Journal Club

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Blog Post

...the questions “Would you attend this journal club again?” and “How would you improve the structure of the...
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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Blog Post

...plasmid from the NeuroMab collection for an antibody against PARIS, a zinc finger protein involved in neurodegeneration...
AAVs in Retinal Gene Therapy

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Blog Post

...emerges from disgrace to be the next big thing, again 3. Acland G. et al., 2001. Gene therapy restores...
Negative Can Be Positive: Open AAV Data with Addgene

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Blog Post

...D-F) High-magnification images immuno-stained against dopamine beta-hydroxylase (DBH, magenta) to label...
Lentiviral Vector Uses and Overview

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Blog Post

...lentiviral vector containing an antisense sequence against the HIV-1 envelope. As of 2013, 65 patients had...
Plasmids 101: Colony PCR

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Blog Post

... complete, it’s time to run the products on an agarose gel to determine their size. Make sure to use an...
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

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Blog Post

...separate plasmids), there are a few reasons arguing against this approach. First, this would prevent the study...
Tips for Screening with Yeast Two Hybrid Systems

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Blog Post

... possible to screen libraries of bait and prey against each other at the same time in order to try and...
Tips from the Repository Trenches: Using Barcodes to Track Samples

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Blog Post

...track your samples today and never lose a sample again! Amanda Hazen is the Laboratory Operations Manager...
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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...genome functions in the hope of developing drugs against intractable diseases. Currently, a number of labs...
Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

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Blog Post

...molecular cloning protocols, including making LB agar plates, restriction digest of plasmid DNA, and bacterial...
Negotiating Work and Life: How to Find the Joy

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Blog Post

...children, so my life will be under control.” Think again. Maybe you’ll need to care for an elderly family...
Plasmids 101: Shuttle Vectors

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...synthesis, and the neomycin resistance gene protects against any of them. But different aminoglycosides work...
CRISPR 101: Any Base Transversion Editors

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...other transversion editors be close at hand? Once again, this post has a lot of acronyms, so we've included...
Tips for Writing a Good Cover Letter

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...manager may have. The closing paragraph should again show your enthusiasm for getting the position and...
Plasmids 101: Broad Host Range Plasmids

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Blog Post

...RepB) necessary for replication in various hosts (Pagatto and Dillon 2001). The plasmid has its own replication...
Bioinformatics at Addgene

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Blog Post

...QC'ing data at Addgene is like standing under a Niagara Falls sized rainbow Over the years we have...

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