We narrowed to 402 results for: CAPS;

1. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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   ...patients with GNB1 mutations, the lab hopes to capture the signalosome of different receptors upon stimulation...

2. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   ... delivery mechanism, but their small packaging capacity of 4-5 kb limits the delivery of larger transgenes...

3. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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   ... the aforementioned methods only determine the capacity of homodimerization in aqueous solution. In cyto...

4. Developing Lab Management Software for Biology

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   ...materials make everyone's life easier, the time and capital cost of getting up to speed with a setup is almost...

5. Gendered Innovations: Why Does Sex of the Cell Matter?

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   ...that there are sex differences in the therapeutic capacity of stem cells. Yet, very few researchers consider...

6. Antibodies 101: Conjugation

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   ...-based assays. Biotin itself is not visible or capable of producing a visible signal, but it has a strong...

7. Plasmid Tools for Microbiome Studies

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   ...Pamela Silver’s lab developed a synthetic mark and recapture strategy for bacteria to count cell divisions....

8. Plasmids 101: Golden Gate Cloning

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   ...precision cloning method with high throughput capability. Engler C, Kandzia R, Marillonnet S. PLoS One...

9. Career Coaching for Scientists: Why and Where Do I Find One?

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   ...transition is also an area of specialty for Michelle Capes at Adeptify Me. While based in Madison, Wisconsin...

10. CRISPR in the Clinic

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    ...Clinical trials are underway to explore CRISPR’s capacity for both classes of edits in cancer therapy. Blood...

11. Viral Vectors 101: Calcium Sensors

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    ...studying the brain, muscles, or intracellular landscape, remember: Keep Ca+2lm and Ca+2rry on! References...

12. Viral Vectors 101: Chemogenetics

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    ...expressed in the brain, it is necessary to use ligands capable of crossing the blood-brain barrier.  Another consideration...

13. Design Tips for Prime Editing

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    ...but this also allows certain unintended edits to escape cellular surveillance. To limit unintended incorporation...

14. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    ...synthetic polyadenylation (spA) signal. Figure and caption adapted from Hazan and Bester 2021.    Alternative...

15. Making CRISPR Plasmids Using Fragmid

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    ...Sizes of fragments are not to scale. Figure and caption from McGee et al., 2024, used under the Creative...

16. Antibodies 101: Stripping and Reprobing Western Blots

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    ...Buffer SDS 2% (w/v) Detergent Β-mercaptoethanol (βME) 100–115 mM = 0.7%–0.8% (v/v) Breaks...

17. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    ...Description Article PI Blue EBFP2 EBFP2(1-10) and Capri(1-10) for use with GFP(11) Multiplexed labeling ...

18. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ...Sequencing (CPS) workflow and yield sufficient data to capture the entire viral genome. The full details of that...

19. How to Prepare for an Industry Interview

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    ...a theme too, which is something not inherently captured by the STAR method. I like to think of the theme...

20. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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    ...focused on modifying the outside protein shell, or capsid, of the virus so that it only enters and delivers...

21. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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    ...the authors tracked protein activity while also capturing information about spatial and temporal organization...

22. Engaging with science and society at pgEd

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    ...briefs for policymakers. The rapidly changing landscape of genetics and our consistent stream of events...

23. Tips for Technical Support Calls

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    ... science, but we don’t have the resources to recapitulate the experiments performed by our thousands of...

24. Adapting Toehold Switches to Detect Zika Virus

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    ...technologies that has transformed the assay’s capabilities. Specifically this includes primer directed ...

25. Plasmids 101: Modular Cloning

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    ...has been used to assemble multigene constructs capable of performing production and subsequent necessary...

26. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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    ...fused to CBP, an histone acetyltransferase domain capable of rearranging chromatin structure. This method...

27. Of Myc and Men

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    ...of antibodies as tools allowed us to detect and capture proteins of interest to better study them in vivo...

28. Hot Plasmids: Winter 2025

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    ...PE2max-NC and ePEmax3 optimized for dicots. NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right...

29. Degrading DNA with Cascade-Cas3

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    ...through DNA degradation, thanks to the unique capabilities of Cas3 and its partner in crime, Cascade. Find...

30. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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    ...anti-Myc antibodies bound to an ELISA plate to capture Myc-tagged sybodies. Then, the biotinylated target...

31. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    ...residues D10 and H840. To ensure that the clones were capable of cleavage, they tested the clones against an ...

32. Transferable Skills Guide: Managing a Team

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    ..., find out what their learning styles are and capitalize on them for the benefit of the team. By properly...

33. Academic vs. Industry Postdocs

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    ...academia, however, writing papers and grants is an inescapable and essential part of being able to continue ...

34. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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    ...undifferentiated “stem” state and, Their potency or capability to differentiate into specialized cell types ...

35. 3D Printing Meets CRISPR Cas9

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    ... of Cas9 – primed for cleavage. This structure captures the displaced non-target DNA strand and shows ...

36. Plasmids 101: Protein Expression

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    ... work with than mammalian cells, and are often capable of modifying complex proteins unlike bacterial ...

37. Google Forums Round Up: First Impressions of NgAgo

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    ...institutions recently reported their inability to recapitulate the results of Gao et al 2016 in a letter to...

38. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    ... YFP, cyan and yellow variants of EGFP equally capable of dimerizing as EGFP. As a result, CFP and YFP...

39. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    ...other people to do that too. We don’t have the capacity to send tools out to people ourselves, but we’...

40. Which Fluorescent Protein Should I Use?

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    ...wavelength. Thus, FPs are proteins with this unique capacity. Many of these FPs are fluorescent when ectopically...