... change across the membrane of cardiac cells. Similarly, neuronal signaling is carried out by action potentials...detailed below. Voltage Sensitive Domain (VSD) GEVIs Simply put, VSD-based sensors consist of a VSD fused to...

... tends to go something like this:   However, as simply as it seems laid out above, there are many places...potential programs and universities stressful or simply not feasible. Through the virtual systems created...

...administered through the tail vein — many researchers will simply refer to these as “tail vein injections.” However...Intranasal instillation Intranasal instillation is very similar to its intratracheal counterpart, as both procedures...

...see if any other fluorescent proteins display a similar effect (many do). Fusing EGFP to the self-labeling... tools, they’re optimistic that MagLOV can be similarly adaptable into future magnetogenetic tools.  Embracing...

...non-transformed cells yielded rates of targeted mutagenesis similar to those achieved by isogenic AdVs encoding TALENs...a potential platform that could be realized via simple switching of the relevant gRNAs and donor DNA Adenoviral...

...vectors can be used to tag endogenous genes and simultaneously introduce a selection marker for screening ... CRISPR/Cas Plasmids - Protein Tagging. Beyond simply monitoring protein localization and activity, CRISPR...

...figure below). The double-MMEJ strategy looks very similar to HDR, but it is accomplished using much smaller... of MMEJ. This double MMEJ strategy looks very similar to HDR, but it is completed using much shorter ...

...over previous genome editing techniques is its simplicity and versatility. CRISPR/Cas9 consists of two ...Pooled lentiviral CRISPR libraries (referred to simply as “CRISPR libraries”) consist of a heterogeneous...

...gene that is expressed, multicistronic vectors simultaneously express two or more separate proteins from ...reporter cloned in. In these plasmids you would simply clone your gene of interest into the multiple cloning...

...forward-engineering the central dogma across many genes simultaneously. These parts have been carefully engineered...incredible hidden story here. The parts we are releasing simply plug into this existing economic activity. People...

...for 8th graders was a little intimidating. Too simplistic and I would insult the students. Too complicated... mechanism, I was careful to keep it relatively simple. I honed the complexity of the talk by running ...

...random association between FPs independent of PPIs. Simply compare the fluorescence intensity of your BiFC...whereas BiFC is useful in scenarios that call for a simple, yes-no answer — i.e., do two proteins interact...

...non-scientist friends and family is Part Five, called simply “Bad Science.” Zimmer kicks off the section saying...provides several. In Part Three of the book, called simply “Old Science,” he describes the now infamous tale...

...to allow those who could not attend a meeting to simply stay caught up and to allow those who may be interested...own knowledge of the field. Perhaps interest has simply waned. When attendance slumps below a certain level...

...sample(s) in the same buffer if possible, or very similar buffers if not, since buffer components can affect...only the linear portion. This allows you to use a simple linear regression trendline, which may be good ...

...discriminate between non-essential and essential genes similarly to the Brunello knock-out library while mitigating...between 150-75 nucleotides upstream of the TSS. Similar to the Brunello and Dolcetto library, sgRNAs were...

... insert and one primer anneals to the insert. A simple way to create this type of primer pair is to mix-and-match...  Primer Design Pros Cons Insert-Specific Simple "yes or no" test results Doesn't tell you the...

...doors. It would be a nightmare if we didn’t have a simple way to track all of our 40,000 samples available...  If a plate of samples is ever dropped, we can simply scan the tubes and know what is in each one. Even...

...and dissemination of experimental protocols, simultaneously allowing scientists to actively comment, annotate...have a record of your work. This feature further simplifies lab work if you like to bring your phone in the...

...Eventually I learned that it’s practical because there simply isn’t enough time to read every single paper. I...present it all in one place to get a sense of the similarities and differences among proteins in the class....

...infections, we are treating microbes with compounds similar to those they have been battling for centuries.... that will be more difficult to treat later. A similar situation can occur if you treat a bacterial infection...

...healthcare consulting firms can be involved in many similar activities depending on the expertise of the senior... to a community of colleagues who are pursuing similar jobs and non-academic careers. Now, more than ever...

...to use the ICE software tool is quick and easy. Simply upload your Sanger sequencing files and provide...analysis, which compares hundreds of samples simultaneously.  The current version of ICE software can analyze...

...established partnership. If you’re willing to do similar things for your team or lab, you’re likely to be... of interacting and is much more effective than simply sending a message asking how things are going. ...

...to two fold for the final titer value, which is simply due to the noise of the assay. In other words, ...). However, because purified AAV preps contain similar and low levels of background protein and chemical...

... in different locations all over the world. But simply attending the conference is usually not enough....young professionals will never attend a job fair! Similar to a conference, job fairs bring you face-to-face...

...chalk talk? On the surface, a chalk talk is quite simple. It's you, a dry erase board, some markers (chalk...chalk talk Perfect your story and delivery. Design simple figures and diagrams to illustrate your vision....

...in mammalian cells. SaCas9 produced indels at a similar efficiency to SpCas9, leading the group to focus...two proteins, mediates protein trans-splicing, similar to an intron in pre-mRNA splicing. In 2016, Fine...

...so great. If we all came from similar backgrounds, we would have similar cognitive biases and this would...

...executed on Linux, we created our first process by simply copying and pasting pieces from our existing script...existing script into the section of the next process. Similar to our first attempt, we manually inspected the...

...maintaining a stable concentration of plasmid. Similar to replication in E. coli, regulation primarily...replication of the plasmid proceeds (and terminates) similarly to the bacterial chromosome. Since replication...

...different RT enzymes and performed large edits with similar or better efficiency than PEmax, the current state-of-the-art...also make CAR-T cell therapy more accessible by simplifying the production process and could even enable ...

...from the cell. Steps 1-1 and 1-2 can be done simultaneously. Image from Li et al., 2021.   The updates.... The optimized plasmid curing method was much simpler and took only ~32 hours instead of ~60 hours. ...

...amenable to multiplexing; expressing 5 gRNAs simultaneously produces comparable gene knockdown to single...LwaCas13a to shRNA, they found that LwaCas13a displays similar knockdown efficiency but no significant off-target...

...work in cell culture. The problems with cell lines Simply put, the majority of cell line problems come from...monitor any changes. Your tracking system could be as simple as a piece of paper on the front of the incubator...

...clones of the original cell. The plasmid DNA is similarly referred to as cloned DNA, and this process of... vary broadly in their functionality. In their simplest form, plasmids require a bacterial origin of replication... DNA fragments. Cloning vectors tend to be very simple, often containing only a bacterial resistance gene...so they can be distinguished from one another. Similar to DNA replication, the Sanger sequencing reaction...

...Scientists use deep mutational scanning to simultaneously test how multiple amino acid changes affect...functional screen of your liking. This method is similar to site-directed mutagenesis (SDM) PCR, but with...

...cloning of your gRNA of interest. Figure 1: Simplified schematic of agrobacterium-mediated transformation...Shan Q, Zhang Y, Liu J, Gao C, Qiu J-L (2014) Simultaneous editing of three homoeoalleles in hexaploid ...

...Reproducibility Project: Cancer Biology have shown similarly disappointing rates of reproducibility. These ...approach the situation differently, some adopting simple note-taking tools, others using excel sheets or...

... enough for all applications. To resolve this, similar systems have been developed for tracking various...animals only permits visualization of two colors. Similarly, Flybow adapts Brainbow 2.0 for use in Drosophila...

... or co-expressed with avidin or a structurally similar protein that was discovered in the 1960s, strepavidin... can label a protein of interest with biotin by simply creating a fusion in which AviTag is added to either...

...this step as well. Preservation and Imaging Similar to Sample Preparation, this step can involve multiple...steps used in a non-fluorescent IHC/ICC assay are similar to the ones you plan to use, then the primary antibody...

...cell spent passing through the laser beam. A is simply the area under the resulting curve. By default,...for both markers. When comparing two markers simultaneously, we commonly describe the resulting populations...

...need to know the exact quantity of your POI, but simply whether its concentration changes in response to...controls are equally strong, so the normalized value simply reports which POI band is stronger. The right gel...

... and note how they build suspense. Notice the simplicity of their slides and how the slides complement...cause and effect. Stories can and should be very simple. The goal is to get the listeners to be thinking...

...program to design primers for Gibson Assembly. For a simple example of using Gibson assembly, imagine that ...size, and purify the DNA fragments. Finally, you simply incubate these three fragments together along with...

...transcript constituted 59% of total dystrophin mRNA, similar to the 39% observed by Tabebordbar et al. Long ...therapy, it’s important to realize that DMD is a simpler case than other genetic diseases we’d like to treat...

...consumables (such as pipet tips, glassware, etc), get a simple list from your current P.I., lab manager, or administrator...need.  Prioritizing resources in your budget The simple practice of putting together a resource budget ...

...assays that have multiple proteins to detect simultaneously. Conjugation The process of attaching signaling...conjugate or looking for antibodies validated for a similar assay type) and search again. After filtering, ..., directly compare the product information for similar antibodies, looking for ones that have identical...price, shipping availability, ease of purchase, or similar factors. Secondary antibodies Secondary antibodies...

...advantages and disadvantages. Sanger sequencing is simple and the most accurate method as it directly reads...predominant state of registers in a sample based on a similarity metric that we defined in our paper. In our opinion...