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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,028 results for: ELL

Showing: 141 - 160 of 1028 results

  1. AAV Viral Preps

    Type
    Collection
    ... tools that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments...Tetracycline Transactivators, Affinity Purification, Cell Ablation, CRISPR Monosynaptic Neuronal Tracing Rabies...

  2. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...sequence. DSBs are preferentially repaired in the cell by non-homologous end joining (NHEJ), a mechanism...PAM site. When a repair template is present, the cell may repair a DSB using homology-directed repair ...

  3. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...Can I directly transfect cloning grade DNA into cells? Cloning grade DNA is designed for use in cloning...cloning applications. Direct transfection into cells is not recommended. Can I order more than 2 µg of cloning...

  4. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ...Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics Background In vivo ...stable imaging of protein interactions in living cells and live mice. Proteins for in vivo imaging have...

  5. Plasmid Collections

    Type
    Collection
    ...Mammalian RNAi Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm ...BioBricks Foundation BRAIN Initiative Collection Cell Migration Consortium Impact of Genomic Variation...

  6. Chemogenetics Plasmids

    Type
    Collection
    ...allowing a scientist to control signaling within cells expressing these engineered receptors. Chemogenetic...engineered receptor is coupled to and the specific cell type where the receptor is expressed, these receptors...

  7. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...then be used to infect permissive cells that express ACE2, the cellular receptor for SARS-CoV-2, in a single...

  8. CRISPR Plasmids - Base Edit

    Type
    Collection
    ... inosine, which is treated like guanosine by the cell, creating an A to G (or T to C) change. Adenine ...hypermutation) in a wider editing window, and are thus well suited to directed evolution applications. Examples...

  9. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...activation activity of three-fold or greater in a well-characterized bacterial two-hybrid reporter system...efficient gene modification. Maeder ML et al. Mol Cell. . 2008. Jul 25. 31(2):294-301. PubMed PMID 18657511...

  10. AAV Packaged on Request

    Type
    Collection
    ... tools that effectively transduce many mammalian cell types. AAV is used in a wide range of experiments...distributed preps around the globe and shared our well-developed and trusted protocols and peer-reviewed...

  11. The Pleiades Promoter Project

    Type
    Collection
    ...therapies for brain disorders by enabling region- and cell-specific gene-delivery in the mouse brain through...Black SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen Y, Cheng JC, Chopra V, Docking...

  12. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Cas protein and a target-specific gRNA in the same cell at the same time. Single plasmids containing both...Alternatively, gRNA-only plasmids can be used in cells or organisms in which Cas9 has been integrated into...

  13. Neuroscience

    Type
    Collection
    ...application-specific validation and quality control by Addgene as well as by our trusted partner labs. To learn more about...

  14. TREAT-AD Plasmid Collection

    Type
    Collection
    ...emerging targets in AD and creating high-quality, well-characterized lead molecules for prioritized targets...

  15. CRISPR Guide

    Type
    Guide
    ...with Haploid Embryonic Stem Cells Carrying a Guide RNA Library. Cell Stem Cell . 17 (2), 221–232. PMID: ...in the same cell. This is called multiplexing , and increases the likelihood that any cell containing ... the cell along with the gRNA(s) and Cas enzyme. The donor DNA contains the desired edit as well as additional...can increase efficiency. Synchronizing cells or modulating the cell cycle can help as HDR takes place during...expressing stable cell line. After applying your screening conditions to your transduced cell population, you...tracking of RNA in live cells, including orthogonal RNA/DNA labeling in the same cell. Figure 11: Overview...promiscuity is unique to bacterial cells and does not occur in mammalian cells. SHERLOCK , developed by the ...

  16. TALEN Expression Vectors

    Type
    Collection
    ...listed below have the CMV promoter for mammalian cell expression and a T7 promoter for in vitro transcription...

  17. Lentiviral Vector Guide

    Type
    Guide
    ...production cell line, typically human embryonic kidney 293T (HEK293T) cells. Standard HEK293T cells are sufficient...fluorescence-activated cell sorting (FACS) is used to sort cells expressing GFP and later expanded into a cell line. More...in a variety of cell types and models. Lentiviral vectors can target non-dividing cells, enable stable ...actively dividing cell types, whereas gamma-retroviruses can only infect mitotically active cell types. This...transfected into HEK293T cells using your preferred transfection method. The HEK293T cells will produce the viral..., and Wells et al. (2020) for examples). Pseudotyping Tropism dictates which types of host cells the lentiviral...make stable cell lines, VSV-G is often put under an inducible promoter. Depending on the cell type, there...

  18. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...based on other human cell lines, such as HeLa and A549 cells, and even insect Sf9 cells , have also been ...able to infect cells and replicate autonomously. Human embryonic kidney 293T (HEK293T) cells are the most...HEK293T cells are then extracted and purified for their use as viral vectors to deliver genes to cells in ...persist in non-dividing cells for a period of years without damaging the host cell. Episomal stability allows...broad range of cell types in vivo. AAV-DJ has been optimized further for specific cell and tissue types...co-delivered to the host cells. The full-length transgene is later reconstructed within the cell via different mechanisms... Movement of molecules, vesicles or organelles from the main cell body, or soma, towards the synapse at...

  19. Antibody Guide

    Type
    Guide
    ... produced. Cells - Antibodies can also be produced in cell cultures using hybridomas, cell cultures created...created by fusing B cells with myeloma cells (immortalized B cells). The genetic reorganization that creates...identify and sort cells from a mixed population. Overview of application Collect cells from cell culture or ...specificity occurs in the B cell itself prior to antibody production; therefore, each B cell only produces one ... specific hybridoma cells into plasmids. Plasmids are then transfected into cells and induced to express...determines the isotype of the antibody. B cells, the immune cell responsible for producing antibodies, can...mature B cell can clone itself to enable higher production of a single antibody, but each B cell can only...

  20. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...-activated cell sorting (FACS) is used to sort cells expressing GFP and later, sorted cells are expanded...actively dividing cell types, whereas gamma-retroviruses can only infect mitotically active cell types. This...Using Packaging Cell Lines Gamma-retroviral vectors can be packaged using helper-free cell lines (Figure...-retroviral packaging cell line developed by Garry Nolan at Stanford. These cell lines contain gag-pol...and rat cells), or an amphotropic envelope, Phoenix-AMPHO (for the infection of mammalian cells). Using...make stable cell lines, VSV-G is often put under an inducible promoter. Depending on the cell type, there... — T and B cell transduction Murine Leukemia Virus (MuLV) and HIV glycoprotein — CD4+ cell transduction...
Showing: 141 - 160 of 1028 results