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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 928 results for: Ina;

Showing: 141 - 160 of 928 results

  1. AAV Viral Preps

    Type
    Collection
    ...Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre Biosensors Calcium Sensors, Glutamate...

  2. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...each plasmid for more information on how it was originally used. Here are a few questions to consider when...

  3. Serotype Testing AAV

    Type
    Collection
    ...which is a sample of the purified DNA from the original plasmid that was used to make the virus. For more...

  4. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...including codon optimization, deletion of the C-terminal 18-21 amino acids and a D614G change which is ...

  5. CRISPR Plasmids and Resources

    Type
    Collection
    ...multiple systems. RNA Editing Cas13 enzymes fused to deaminases can edit bases in RNA. Other Applications Screen...

  7. AAV for Neuronal Tracing

    Type
    Collection
    ... are naturally expressed at presynaptic nerve terminals. (4) Neurons projecting to the starter cell are...

  9. Retrovirus Plasmids

    Type
    Collection
    ...overexpression plasmid; deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation...

  10. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...prime editing. Created with BioRender.com. The original prime editing enzyme is named PE1 . PE1 and most...

  11. CRISPR Plasmids - Plants

    Type
    Collection
    ... Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific cytosine base editor...

  12. Chemogenetics Guide

    Type
    Guide
    ...optogenetics workflows (such as laser or LED illumination), making LMOs a versatile option. Coupling brighter...Suhara T, Takada M, Higuchi M, Jin J, Roth BL, Minamimoto T (2020). Deschloroclozapine, a potent and selective...

  14. Optogenetics AAV Preps

    Type
    Collection
    ...-eYFP CAG ChR2/H134R EYFP Cre dependent PHPeB Gradinaru 107708 pAAV-hSynapsin- soCoChR-GFP Syn CoChR (...

  15. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...in mammalian cells. sin 3’LTR 3’ Self-inactivating long terminal repeat. f1 ori f1 bacterial origin of... Map of pLKO.1 containing an shRNA insert. The original pLKO.1-TRC cloning vector has a 1.9kb stuffer ...the transduced cells. hPGK Human phosphoglycerate kinase promoter drives expression of puromycin. Puro R...bacterial origin of replication. 5’LTR 5’ long terminal repeat. RRE Rev response element. A.3 Related ...repeats, especially repeated Ts because polyT is a termination signal for RNA polymerase III. Note that these...and ligated into the pLKO.1 vector, producing a final plasmid that expresses the shRNA of interest. C.1...buffer 1 1 μL AgeI add ddH 2 O to bring to 50 μL final volume Incubate at 37°C for 2 hours. Purify with...

  16. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...protocol is composed of steps that separate out contaminants from an impure AAV preparation. The 15% iodixanol...macromolecules. The 40% and 25% steps are used to remove contaminants with lower densities, including empty capsids...mg of MgCl 2 and 14.91 mg of KCl in 1× PBS in a final volume of 100 mL. Sterilize by passing through a... of MgCl 2 , and 14.91 mg of KCl in 1× PBS in a final volume of 100 mL. Sterilize by passing through a....5 mL to 1 mL fractions per tube. Avoid the proteinaceous material near the 40–25% interface. Pro-Tip ...per tube, taking care to avoid collecting the proteinaceous material at the 40–25% interface. Repeat for.... Image adapted from Zolotukhin, S., et al. "Recombinant adeno-associated virus purification using novel...

  17. Lentivirus ddPCR Titration

    Type
    Protocol
    ...mL pipette 5–10 times. The final volume in the well is 1.5 mL, and the final Polybrene concentration is...high glucose, pyruvate, Corning, 10-013-CV Heat-inactivated FBS L-alanyl-L-glutamine (or alternative stable...500 mL DMEM, high glucose, pyruvate 55 mL heat inactivated premium grade, fetal bovine serum 5 mL glutaGRO...new aspirating pipette for each well as to not contaminate cell conditions with others. Gently add 1.5 mL...master mix for 9 samples. Component Volume 9X Volume Final Concentrations 2X ddPCR Supermix for Probes, no ...from the appropriate dilutions to calculate the final infectious titer. Tips and Troubleshooting We recommend...freezer blocks during use. To reduce the risk of contaminating reagents we recommend making small aliquots ...

  18. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...When to Use Them Immunocytochemistry Protocol Recombinant Antibody Purification Protocol Antibody Guide... software Reagents Purified antigen Purified recombinant antibody Isotype control antibody HRP-conjugated...10 twice for a total of three washes. After the final wash, aspirate the wash buffer from the wells. Prepare...20 twice for a total of three washes. After the final wash, aspirate the wash buffer from the wells. Prepare...32 twice for a total of three washes. After the final wash, aspirate the wash buffer from the wells. Dilute...42 twice for a total of three washes. After the final wash, aspirate the wash buffer from the wells. Immediately...to factor in that dilution when calculating the original sample concentration!). Sample Data Figure 1: ...

  19. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...method for decontaminating must be used for proper disposal. Liquid BSL-2 waste can be decontaminated in a ...biohazardous waste. Dispose of waste that may be contaminated with pathogens or chemicals that present a danger...biohazard containers. Biohazardous materials are decontaminated prior to disposal, commonly done with an autoclave...underneath a hood. Clean any spills immediately, and decontaminate as necessary. For large spills that cannot be...participating in BSL-2 work receives a hepatitis B vaccination or titer prior to starting work in the laboratory...a final concentration of 10% bleach for 30 minutes before pouring down the drain. Solid BSL-2 waste can...

  20. CRISPR Library Amplification

    Type
    Protocol
    ...Bottlenecks, fitness differences, and plasmid recombination can all impact the representation of individual...libraries may require modifications dictated by the originating laboratory for optimal results. If you obtained...replication and antibiotic selection). This recombination, at a low rate, is not typically a problem as...lentiviral particles. See below for options if the recombinant band makes up a significant proportion of the...electrocompetent cells that are suitable for unstable or recombination-prone DNA. The use of electrocompetent cells...step. I got a heavy recombined band by digest. Recombination is thought to be in part related to selective...burden on the bacteria. Bacteria that contain a recombinant plasmid likely grow faster in the closed system...
Showing: 141 - 160 of 928 results