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We narrowed to 952 results for: OMP;

Showing: 141 - 160 of 952 results

  1. General Transfection

    Type
    Protocol
    ...DMEM complete containing 25 µM chloroquine diphosphate and incubate ~5 h For 10 mL of DMEM complete, add...Syringes for filtering Reagent Preparation DMEM Complete: 10% v/v FBS and 4 mM L-alanyl-L-glutamine (or...HEK293T cells at 3.8x10 6 cells per plate in DMEM complete in 10 cm tissue culture plates. Incubate the cells...the media. Replace the media with 15 mL of DMEM complete. Incubate the cells 24–48 h before checking for...

  2. Chemogenetics Guide

    Type
    Guide
    ...DREADDs. Compound 21 has similar potency as CNO, while DCZ is more potent than either CNO or Compound 21. ...demonstrating the need for alternative DREADD ligands. Compound 21, deschloroclozapine (DCZ), perlapine, and olanzapine.... Both Compound 21 and DCZ seem to have minimal off-target activity, and do not seem to have the same ...10.1073/pnas.95.1.352 PMID: 9419379 Davies, M. A., Compton-Toth, B. A., Hufeisen, S. J., Meltzer, H. Y., &..., Richie, C. T., Harvey, B. K., Dannals, R. F., Pomper, M. G., Bonci, A., & Michaelides, M. (2017). Chemogenetics...

  3. Optogenetics Guide

    Type
    Guide
    ...H134 Widely used variant with larger photocurrent compared to CrChR2 450 ChETA E123T mutation; creates faster...expression. These plasmids rely on the engineered two-component regulatory system YF1/FixJ. YF1 is a synthetic... 17035522 Dugué GP, Akemann W, Knöpfel T. A comprehensive concept of optogenetics. 2012 Prog Brain Res...Ramakrishnan C, Mattis J, Prakash R, Diester I, Goshen I, Thompson KR, Deisseroth K. 2010 Molecular and cellular ...applying optogenetic tools derived from direct comparative analysis of microbial opsins. Nat Methods. 9(...K. 2011. Microbial opsins: a family of single-component tools for optical control of neural activity. ...

  4. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...Refrigerator Pipette controller Benchtop centrifuge compatible with 50 mL conical tubes NanoDrop spectrophotometer... 250 mL sterile bottle, VWR 430281 0.45 µm PES complete filtration unit, 500 mL, rapid-flow, VWR 73520...Place a mark on the side of the column where the compacted resin is slanted upward. Place column in centrifuge...centrifugation step Pro-Tip Resin will appear compacted after centrifugation. Discard the flow through.... Slowly apply the sample to the center of the compact resin bed. Pro-Tip For sample volumes <1.5 mL top...

  5. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...denaturation, annealing, and synthesis. After the PCR is complete, the product can be verified based on size by ...Diluting Primers Most people order primers from a company, which synthesizes and ships them as a lyophilized...essential that the template DNA and primer are complementary for a PCR to work efficiently. Taq Buffer with...Forward and Reverse Primers: Hybridize and are complementary to the 3’ ends of the sense and anti-sense strands...

  6. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...Corning 430167 (optional) Reagent Preparation DMEM Complete: 10% v/v FBS and 4 mM L-alanyl-L-glutamine (or...Each 10 cm dish should be seeded in 10 mL DMEM complete, which will generate enough conditioned medium...conditioned medium, prepare this cell solution in fresh complete medium. This 5 cells/mL solution will be used ...adding 10 µL homogenized cell solution to 990 µL complete medium. Instead of transferring 0.125 µL, now ...

  7. AAV ddPCR Titration

    Type
    Protocol
    ...layout is complete, select 'Run' to begin the droplet reading. When the droplet reading is complete, export... on the insert. Please ensure that you are in compliance with your institution’s biosafety regulations...Stabilization 98 10 2 1 Hold 4 ∞ 2 1 After PCR is complete, transfer the plate to the Droplet Reader. Open...

  8. Ligation Independent Cloning

    Type
    Protocol
    ...restriction digest protocol ). After the digest is complete, you will need to separate the linearized vector... A typical T4 Pol reaction is shown. Mix the components (add the T4 last) and incubate at room temperature...of T4 Pol in the following step. This may be accomplished by gel purification . Step 5: Create Insert ...

  9. Centrifugation

    Type
    Protocol
    ... and using a centrifuge to separate different components in a liquid sample....laboratory equipment used to separate different components in a liquid sample. It achieves this by using... you use, most will have essentially the same components. The photo to the left shows a front view of ...

  10. Gibson Assembly Protocol

    Type
    Protocol
    ...2009). Regardless of fragment length or end compatibility, multiple overlapping DNA fragments can be joined...overhangs by chewing back from the DNA 5’ end. Complementary DNA fragments can subsequently anneal to each... DNA Ligase - covalently joins the annealed complementary DNA fragments, removing any nicks and creating...

  11. Protocol - How to Create a Bacterial Glycerol Stock

    Type
    Protocol
    ...bacterial strain and you will not need to obtain more competent cells and retransform. Bacteria on an LB agar ...streaking onto LB agar will prevent it from thawing completely and will improve the shelf life. It is very important...

  13. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...biosafety level 2. Please ensure that you are in compliance with your institution’s biosafety regulations...tips Pipettor and pipettes Benchtop centrifuge compatible with 50 mL conical tubes Automated cell counter...

  14. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...vivo . The iodixanol gradient in this protocol is composed of steps that separate out contaminants from an...exchange and concentration Note: Both steps could be completed in one (long) day. Equipment Ultracentrifuge T70i...

  15. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...). Microwave for 1-3 min until the agarose is completely dissolved (but do not overboil the solution, ...room temperature for 20-30 mins, until it has completely solidified. Pro-Tip If you are in a hurry, the...

  16. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...bacterial cultures , please see those pages. Several companies, such as (Link opens in a new window) NEB , (Link...but within a smaller volume of buffer that is compatible with the next solution. Add a denaturing solution...

  17. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...science. General Considerations The ELISA can be completed in a single day or broken up over several days...ELISA plate washer. Equipment Spectrophotometer compatible with 96-well plates 1–10 µL single channel pipette...

  18. Kit Free RNA Extraction

    Type
    Protocol
    ...to allow for dissociation of the nucleoprotein complexes. The effectiveness of your RNA isolation will ...

  19. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...plasmid. It shows you each component, with the ID of the component plasmid, component name, size, and any comments...module, are the components. Using the handy website, users can simply select the components they want for...able to select components that work with your design. Note that you can browse all components by clicking...Fragmid doesn’t have the components I want? You can make your own Fragmid components! Ensure your backbone... Each module of Fragmid represents a class of components, such as your CRISPR enzyme or Pol 2 promoter...’ terminus, and selection markers. Need fewer components? A spacer can be inserted instead, allowing everything...everything to stay in the correct order. Most Fragmid components are available for request on the Addgene website...

  20. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...easily design, generate, and test constructs composed of complex genetic elements. To demonstrate the usefulness...several GMAP-compatible vectors for a wide variety of biological applications. First, GMAP-compatible backbones...mammalian systems. The DULIP system is comprised of Gateway-compatible plasmids in which “bait” proteins are...plasmids designed to complement unused auxotrophies in Saccharomyces strains by compensating for histidine (... do so have been hindered by a lack of tools, compounds, and assays to monitor activation of these receptors...including a stop codon) formats. The collection is compatible with both standard Gateway cloning and the FNLCR...assembly platform for generating both simple and complex DNA constructs Have you ever wished you could ...
Showing: 141 - 160 of 952 results