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We narrowed to 399 results for: PREP

Showing: 141 - 160 of 399 results

  1. Optogenetics Plasmid Collection

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    Collection
    ... terms. Looking for viral preps? We also offer ready-to-use AAV preparations of many optogenetics plasmids...

  3. CRISPR Plasmids - gRNAs

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...

  4. CRISPR Plasmids - Cascade-Cas3

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Cas3...

  9. Hot Plasmids: Fall 2024

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    Blog Post
    ...some of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles...

  10. AAV Titers: Where Do They Come From And What Do They Mean?

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    Blog Post
    ...Taylor et al., 2017). However, because purified AAV preps contain similar and low levels of background protein...vector preparations. Addgene has used this method to generally validate our viral vector preparation protocol...al., 2010). The specific infectivity of viral preparations is defined by the ratio of physical viral particles...viral capsids in representative viral vector preparations are genome-containing (Figure 1).    ...Electron micrograph of an Addgene AAV viral vector preparation after negative staining. Empty vector particles...viral service, we currently titrate AAV vector preparations by qPCR. To ensure accurate and reliable results...

  11. Site Directed Mutagenesis by PCR

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    Blog Post
    ...product (see figure). Template A high purity plasmid prep significantly increases the success rate of site...’ll want to steer clear of these strains when preparing the plasmid template for site directed mutagenesis...

  12. Plasmids 101: E. coli Strains for Protein Expression

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    Blog Post
    ... auxillary plasmids may be isolated during the prepping process. How does inducible expression work? As...) General protein expression  m15 pREP4*, ** Kanamycin (pREP4) Cis-repression of the E. coli T5 promoter...similar), inducible under IPTG (lac repressor on the pREP4 plasmid). Expression vector cannot have p15A origin...the strain. Conversely, other strains such as M15(pREP4) use a lac repressor to act directly on the expression...

  13. Chemogenetics Plasmids

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    Collection
    ... terms. Looking for viral preps? We also offer ready-to-use AAV preparations of many chemogenetics plasmids...

  14. CRISPR Plasmids and Resources

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    Collection
    ...Addgene's Viral Service : Request ready-to-use viral preps of select CRISPR lentiviral plasmids. CRISPR Plasmid...

  15. CRISPR Plasmids - Base Edit

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two...

  16. CRISPR Plasmids - C. elegans

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...

  17. Plasmids 101: Common Lab E. coli Strains

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    Blog Post
    ...recombine. This strain is endA+, so column-based DNA preps require an additional wash step. F- glnV44 recA13...cloning, routine cloning, cloning unstable DNA, preparing unmethylated DNA, and more. Many mutations that...Consequence dam DNA adenine methylase mutation (GATC) Preparing unmethylated DNA, important when trying to cut... dcm DNA cytosine methylase mutation (CCWGG) Preparing unmethylated DNA, important when trying to cut...restriction enzymes to cut the plasmid after preparation. rpsL thr leu thi lacY galK galT ara tonA tsx...

  18. Plan Your Experiment

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    Collection
    ...most common. Using viral vectors takes a bit more prep work, as you first have to produce the viral particles...Browse Addgene's collection of in-stock lentiviral preps . AAV vectors AAV vectors offer some advantages ...size limitation . Browse Addgene's in-stock AAV preps or try Addgene's Packaged on Request service. Delivery...

  19. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...
Showing: 141 - 160 of 399 results