We narrowed to 156 results for: ado

1. RNA Interference in Plant Biology: New Tools for an Old Favorite

   Type

   Blog Post

   ...the RNAi transgene, followed by a fluorescence readout of RNAi activity. As a plant researcher, the traditional...

2. Sequencing Options for CRISPR Genotyping

   Type

   Blog Post

   ...electrophoresis. Surveyor™ and T7E1 have been widely adopted due to their relative simplicity and low cost. ...

3. Typing CRISPR Systems

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   Blog Post

   ... notable in recent years. A variant of V-U, paradoxically called V-K, is a CRISPR-associated transposase...

4. Antibodies 101: Designing Your First Flow Panel

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   Blog Post

   ...together, are able to give you the appropriate readout. A common method of labeling proteins is to fuse...

5. Antibody Validation for Flow Cytometry

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   Blog Post

   ...of interest, which can affect your experimental readouts. Be aware that in any version of this approach...

6. Deep Dive: qPCR

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   Blog Post

   ...understand variation due to technical error.  Plate readouts can be affected by plate type or tubes, sealing...

7. 15 Hot Plasmids from 2017

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   Blog Post

   ...these instructions to prepare the PSU ladders! Rosado, et al. Autophagy. 2008. PubMed PMID: 18094608 ...

8. Viral Vectors 101: Systemic Capsids

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   Blog Post

   ...., Greenbaum, A., Ravi, N., Wu, W. L., Sánchez-Guardado, L., Lois, C., Mazmanian, S. K., Deverman, B. ...

9. 27 Hot Plasmids from 2016

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   Blog Post

   ...examine the mechanisms behind autophagic pathways. Rosado, et al. Autophagy. 2008. PubMed PMID: 18094608 ...

10. CRISPR Guide

    Type

    Guide

    ...Theunissen, T. W., Rangarajan, S., Shivalila, C. S., Dadon, D. B., & Jaenisch, R. (2013). Multiplexed activation...Communications , 9 (1). PMID: 30082838 Maddalo, D., Manchado, E., Concepcion, C. P., Bonetti, C., Vidigal, ..., Cogan, J. Z., Replogle, J. M., Adriaens, C., Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J.,...Partridge, E. C., Newberry, K. M., Smith, S. B., Meadows, S. K., Roberts, B. S., Mackiewicz, M., Mendenhall...

11. Chemogenetics Guide

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    Guide

    ...activated by the synthetic ligand spiradoline, however, spiradoline exhibited off-target effects in vivo...

12. Plan Your Experiment

    Type

    Guide

    ...NHEJ repaired DSBs): Provides a semi-quantitative readout of the percentage of alleles that have been mutated...

13. Optogenetics Guide

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    Guide

    ...stimulated. Channelrhodopsin-2 (ChR2), the first widely adopted optogenetic tool, is also a blue light activated...

14. Lentiviral Vector Guide

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    Guide

    ...j.biopha.2020.110276 PMID: 32502836 Johnson, N. M., Alvarado, A. F., Moffatt, T. N., Edavettal, J. M., Swaminathan...

15. Adeno-associated virus (AAV) Guide

    Type

    Guide

    ...., Greenbaum, A., Ravi, N., Wu, W. L., Sánchez-Guardado, L., Lois, C., Mazmanian, S. K., Deverman, B. ...

16. Antibody Guide

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    Guide

    ...Sample preparation, signaling excitation, and assay readout vary widely depending on individual assay conditions...