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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 326 results for: iNOS

Showing: 141 - 160 of 326 results

  6. CRISPR Guide

    Type
    Guide
    ...base editor that converts adenosine to inosine in RNA. Since inosine is functionally equivalent to guanosine...to small indels in the target DNA that result in amino acid deletions, insertions, or frameshift mutations...enzymes. hfCas9s were developed by mutating specific amino acid residues to reduce off-target editing. Some... prime editing, and are often used to change an amino acid, turn a promoter on/off, or add small protein...with a silent mutation that does not change the amino acid sequence. HDR efficiency is generally low (...editors have been engineered to convert adenosine to inosine, which is treated like guanosine by the cell, creating...soon followed, using an enzyme evolved to excise inosine to access the same repair pathway and convert A...

  8. Immunocytochemistry

    Type
    Protocol
    ...24-well plate 4% Paraformaldehyde 5 mg/mL 4′,6-diamidino-2-phenylindole (DAPI) Bovine serum albumin (BSA...

  9. Molecular Biology Reference

    Type
    Guide
    ...within an organism. Proteins are made up of amino acids. Each amino acid is encoded for by 3 nucleotides termed... there are only 20 natural amino acids and 64 codon combinations each amino acid is encoded for by multiple...encodes for an amino acid. The genetic code is degenerate thus multiple codons code for each amino acid. There...mg/mL 200 µg/mL Kanamycin 50 mg/mL 50 µg/mL Spectinomycin 50 mg/mL 50 µg/mL Tetracycline 10 mg/mL 10 µg...There are 20 amino acids plus a start and stop codon. Below you will find helpful resource tables about...genetic code. This table includes the nucleotide and amino acid code in addition to ambiguous bases and common... A or G S C or G V A, C, or G W A or T Y C or T Amino Acids Name Three Letter Code Single Letter Code ...

  10. Deep Dive: qPCR

    Type
    Blog Post
    ...corrections are a better choice (Tichopa, 2003. Marino, 2003) - an important note, as the other most commonly...pitfalls and potential. BioTechniques 26:112–115.Marino JH; Cook P; and Miller KS. 2003. Accurate and statistically...

  11. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...Gootenberg, J. S., Konermann, S., Trevino, A. E., Scott, D. A., Inoue, A., Matoba, S., Zhang, Y., & Zhang...

  13. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...extracellular space. In addition, rAAV2-retro’s altered amino acid sequence might facilitate interaction with ...PubMed PMID: 1381034. Watakabe, A., Ohtsuka, M., Kinoshita, M., Takaj, M.I., Isa, K., Mizukami, H., Ozawa...

  15. Chemogenetics Guide

    Type
    Guide
    ...was developed to respond to a different ligand, Salvinorin B (SALB). Scientists have shown that both KORD...Addgene's collection of PSAM plasmids ! LMOs Luminopsins (LMOs), or luminescent opsins, are opto-chemogenetic...systems in our Optogenetics Guide . Figure 4: Luminopsins can be activated through chemogenetic or optogenetic...Augustine GJ, Yin HH, Hochgeschwender U (2016). Luminopsins integrate opto- and chemogenetics by using physical...Hirabayashi T, Fujimoto A, Mimura K, English JG, Liu J, Inoue K, Kumata K, Seki C, Ono M, Shimojo M, Zhang M-R...Shaner NC, Hochgeschwender U (2024). Engineering luminopsins with improved coupling efficiencies. Neurophotonics..., Gutekunst CA, Gross RE. (2015). Inhibitory luminopsins: genetically-encoded bioluminescent opsins for...

  16. Pouring LB Agar Plates

    Type
    Protocol
    ... mg/mL 10 µg/mL Kanamycin 50 mg/mL 50 µg/mL Spectinomycin 50 mg/mL 50 µg/mL Tetracycline 10 mg/mL 10 µg...

  18. KLF Research Plasmids

    Type
    Collection
    ... metabolism, fibrosis, atherosclerosis, and carcinogenesis. The defining feature of the KLF family is ...

  20. Chemogenetics Plasmids

    Type
    Collection
    ...Another recently developed class of tools are Luminopsins (LMOs), or luminescent opsins: fusion proteins...
Showing: 141 - 160 of 326 results