...target cell type or tissue of interest, and model organism under study. *Many engineered AAVs for mice use...

...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...loci can be easily expressed in the same cell or organism. The ability to precisely target CRISPR to a given...

...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...CI.TurboRFP.WPRE.RBG James M. Wilson Please contact help@addgene.org with any questions. Return to top...

...the sequences, and email the file to deposit@addgene.org with the subject heading "gRNA sequence spreadsheet...

...16), eadg6618. https://doi.org/10.1126/sciadv.adg6618 Stanton, A. C., Lagerborg, K. A., Tellez, L., Krunnfusz..., 31-50.e8. https://doi.org/10.1016/j.medj.2022.11.002 Tabebordbar, M., Lagerborg, K. A., Stanton, A.,...administration, distributing widely throughout the organism to target large or diffuse biological structures...specific application and animal model.  Other model organisms Tropism and efficacy can vary depending on species...target cell type or tissue of interest, and model organism under study. *Many engineered AAVs for mice use...Addgene Capsid Published Tropism Model Organism Mouse Strain-Specific Tropism?  Reference... and shows decreased transduction in off-target organs (Goertsen, Flytzanis, et al., 2022). AAV-PHP.N ...

...different organisms. There are several codons that encode the same amino acid. Each organism, however,...the genetic code is universal, many different organisms actually prefer certain codons over others for... biologist who wants to express genes from one organism in another? Let’s take a look at codon usage and...optimize codons for expression in a particular organism. Why is codon usage important? Translation and...for codon-usage of 257,468 genes across 8,792 organisms (Nakamura et al., 2000).  What is codon optimization...expression by changing synonymous codons based on an organism's codon bias. The idea is that scientists can make...mutations throughout a gene of interest based on an organisms codon usage bias to increase translational efficiency...

...across organisms. When using the MK system, many of the parts can be used in a variety of organisms, saving.... Crossref, https://doi.org/10.1038/s41598-019-46171-2. Resources on Addgene.org Modular Cloning (MoClo... these applications, no matter what your model organism is. MoClo background MoClo is a clever way to...MoClo kit recommendations for different modal organisms and applications.    Plant applications We...libraries and tools available for certain model organisms and backgrounds, MoClo can be invaluable for more... complex editing and screening experiments in organisms that don’t yet have these tools built out. While...many MoClo kits are designed with specific model organisms in mind, others are designed for flexibility. ...

...to mid-sized companies and organizations. Addgene as a nonprofit organization is very considerate and thoughtful... outside an organization The next step is to expand your efforts beyond your organization and/or scientific...people from various organizations in science and beyond. For example, I help organize scientific workshops...guide: Collaborating with others outside your organization also known as external collaborations. Interestingly...the ability to work with others outside one’s organization was found in the aforementioned survey as one... partnerships where we can leverage another organization’s expertise to our mutual benefit.  Our partnership...small: Collaboration opportunities within an organization but outside your comfort zone As part of my ...

...classification While CRISPR systems now seem neatly organized into their respective categories, arriving at ...CRISPR–Cas13. Nature, 550(7675), 280–284. https://doi.org/10.1038/nature24049 Altae-Tran, H., Kannan, S.,...terascale clustering. Science, 382(6673). https://doi.org/10.1126/science.adi1910 Chylinski, K., Makarova...Nucleic Acids Research, 42(10), 6091–6105. https://doi.org/10.1093/nar/gku241 Cong, L., Ran, F. A., Cox, D... systems. Science, 339(6121), 819–823. https://doi.org/10.1126/science.1231143 Cox, D. B. T., Gootenberg...-Cas13. Science, 358(6366), 1019–1027. https://doi.org/10.1126/science.aaq0180 Harrington, L. B., Burstein... enzymes. Science, 362(6416), 839–842. https://doi.org/10.1126/science.aav4294 Hu, C., Myers, M. T., Zhou...

...has taken place in other organisms. In many bacteria, unlike other organisms, CRISPR-induced double stranded...diseases have connections to either specific organisms or organism/gene interactions with the host. This scenario...can consider the microbiome as a collection of organisms, their genes, and metabolic processes, occupying...to diseases where the presence of just a single organism, such as Clostridium difficile, could be targeted... This approach successfully targeted specific organisms when using bacteriophages or mobile plasmids in...Pseudomonas species Expanding this tool to other organisms, the Ji lab brought genome and base editing to...genome engineering remains challenging for most organisms in the genus. Work from the Sun lab demonstrates...

... ease of use and utility in a wide variety of organisms.  While much of current CRISPR research focuses... plant species, including commonly used model organisms like Arabidopsis and Medicago truncatula and several...universal functionality of the CRISPR system in most organisms, some plant-specific changes to CRISPR components...experimental design principles developed in other model organisms (see our CRISPR guide for common CRISPR principles...sequence) is consistent between plants and other organisms, but the promoter used to drive gRNA expression...your gene of interest into a target cell line or organism (Presented in figure 1). More information on Agrobacterium-mediated...CRISPR/Cas system. Plant Methods 9:39 . https://doi.org/10.1186/1746-4811-9-39 Gao Q, Xu W, Yan T, Fang...

...to one or a subset of organisms. Examples: “organism agnostic”, “model organisms”, “small genome”, “large...require the organism to be specified and are truly organism agnostic. Other tools require an organism to be ...only one, or a subset of organisms. In only a few cases the software is organism agnostic. Most tools are...tools will be marked as organism agnostic and have currently available organisms listed in this section...input types can be readily converted.  Examples: “organism”, “sequence”, “identifier”, and “load”. Trends... tools also requiring that the user specify an organism. Output diversity: Different tools provide results...large genome”, and “organism biased scoring”. Trends: Many tools can handle genomes of any size and are...

...Therapy: An Overview. Cells, 12(5), 1–41. https://doi.org/10.3390/cells12050785 Murlidharan, G., Samulski...Frontiers in Molecular Neuroscience, 7. https://doi.org/10.3389/fnmol.2014.00076 References and Resources...the Mouse Brain. PLOS ONE, 8(9), e76310. https://doi.org/10.1371/journal.pone.0076310 Damdindorj, L., Karnan...adeno-associated viral vectors. PLoS ONE, 9(8), 1–10. https://doi.org/10.1371/journal.pone.0106472 de Backer, M. W. A...Hypothalamus. Human Gene Therapy, 21(6), 673–682. https://doi.org/10.1089/hum.2009.169 Dudek, A. M., Pillay, S., ... Receptor. Journal of Virology, 92(7). https://doi.org/10.1128/jvi.02213-17 Favre, D., Blouin, V., Provost...Journal of Virology, 76(22), 11605–11611. https://doi.org/10.1128/JVI.76.22.11605-11611.2002 Griffin, J. ...

...807, 141–157. https://doi.org/10.1007/978-1-61779-370-7_6 Tabebordbar, M., Lagerborg, K. A., Stanton, A....which allow AAVs to broadly spread throughout an organism, including difficult-to-target tissues like the...vector cargo designs and administration doses. Don't forget to submit your validation data after your experiment...the mouse brain. PloS One, 8(9), e76310. https://doi.org/10.1371/journal.pone.0076310 Bish, L. T., Morine...Human Gene Therapy, 19(12), 1359–1368. https://doi.org/10.1089/hum.2008.123 Dalkara, D., Byrne, L. C.,...Translational Medicine, 5(189), 189ra76. https://doi.org/10.1126/scitranslmed.3005708 Goertsen, D., Goeden...Methods & Clinical Development, 26, 331–342. https://doi.org/10.1016/j.omtm.2022.07.010 Issa, S. S., Shaimardanova...

... CRISPR-based genome editing in organoids By Ashley Waldron Organoids are three-dimensional cultures that...toolkit for performing genetics studies in organoids. The Organoid EasyTag System is a straightforward workflow...in an organoid model (Sun, et al. 2021).  Figure 1: Example schematic from the Organoid EasyTag...fetal-lung derived organoid system, the approach is intended to be amenable to organoids derived from other...find in this article: CRISPR-based editing in organoid Biosensors for detecting strigolactones The CRISPR... that mimic aspects of organ morphology and physiology in vitro and have emerged as a powerful model for...disease. In order to expand their model’s impact, organoid users have recently begun to develop CRISPR-based...

... a wide range of organisms and cell lines. In C. elegans, one of the model organisms which I use to study...198:1347–1356 . https://doi.org/10.1534/genetics.114.170423 Schwartz ML, Jorgensen EM (2018) SapTrap vectors...methods – could greatly streamline editing in other organisms and systems, allowing rapid progress in a wide...Caenorhabditis elegans. Genetics 198:837–846 . https://doi.org/10.1534/genetics.114.169730 Bottcher R, Hollmann...cells. Nucleic Acids Research 42:e89–e89 . https://doi.org/10.1093/nar/gku289 Chen C, Fenk LA, de Bono M (... Nucleic Acids Research 41:e193–e193 . https://doi.org/10.1093/nar/gkt805 Dickinson DJ, Pani AM, Heppert...Selection Cassette. Genetics 200:1035–1049 . https://doi.org/10.1534/genetics.115.178335 Dokshin GA, Ghanta ...

...genetic code is used by most organisms on Earth, but different organisms have different preferences for...codons are used preferentially in which organisms - organisms have codon usage bias. You can find many...peptide of interest and produce that peptide in your organism of choice. Unfortunately, codon preferences make...and expect your sequence to express well in any organism. So what are the evolutionary constraints that... do about them? Read on to find out!   Why do organisms have different codon usage biases? The reasons...reasons for varied codon preferences among organisms aren’t completely understood, but some possible reasons... tRNAs with the appropriate amino acids. If an organism uses only a subset of codons, it only needs to...

... fluorescent protein somebody made because they forgot it was already in the freezer… you get the idea...Various Many Complicated   Applications Organic dyes are typically both brighter and more photostable...imaging of ER and mitochondria reveals dynamic organelle interactions. a) Labeling scheme. b) COS-7 cell...at a price, though. Purchasing lots and lots of organic dyes or specialized ligands is more expensive than...ligands brings up bad memories of undergraduate organic chemistry lab, don’t worry – many SNAP-tag and ...Nature Communications, 7(1), Article 1. https://doi.org/10.1038/ncomms10778 Erdmann, R. S., Baguley, S....Cell Chemical Biology, 26(4), 584-592.e6. https://doi.org/10.1016/j.chembiol.2019.01.003 Gallagher, S. S....