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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 949 results for: org

Showing: 141 - 160 of 949 results

  1. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Ma T, Ztaou S, Sienna AC, Tepler S, Poulin JF, Ansorge M, Awatramani R, Kang UJ, Rayport S. 2018. Dopamine...

  2. Promoters

    Type
    Guide
    ...promoter region controls when and where in the organism your gene of interest is expressed. Summary Promoters...

  3. The Pleiades Promoter Project

    Type
    Collection
    ...Natl Acad Sci U S A, 107 (38):16589-94. https://doi.org/10.1073/pnas.1009158107 (Link opens in a new window...

  4. Caltech Systemic Capsids

    Type
    Collection
    ...target cell type or tissue of interest, and model organism under study. *Many engineered AAVs for mice use...

  5. Sequencing Primers

    Type
    Guide
    ... sure what primer you need? Email us at help@addgene.org Addgene has used a number of primers for sanger...

  7. Optogenetics Guide

    Type
    Guide
    ...and study certain behavioral responses in model organisms like mice, zebrafish, and Drosophila. These tools...

  9. Validated gRNA Sequences

    Type
    Collection
    ...the sequences, and email the file to deposit@addgene.org with the subject heading "gRNA sequence spreadsheet...

  10. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...16), eadg6618. https://doi.org/10.1126/sciadv.adg6618 Stanton, A. C., Lagerborg, K. A., Tellez, L., Krunnfusz..., 31-50.e8. https://doi.org/10.1016/j.medj.2022.11.002 Tabebordbar, M., Lagerborg, K. A., Stanton, A.,...administration, distributing widely throughout the organism to target large or diffuse biological structures...specific application and animal model.  Other model organisms Tropism and efficacy can vary depending on species...target cell type or tissue of interest, and model organism under study. *Many engineered AAVs for mice use...Addgene Capsid Published Tropism Model Organism Mouse Strain-Specific Tropism?  Reference... and shows decreased transduction in off-target organs (Goertsen, Flytzanis, et al., 2022). AAV-PHP.N ...

  11. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...different organisms. There are several codons that encode the same amino acid. Each organism, however,...the genetic code is universal, many different organisms actually prefer certain codons over others for... biologist who wants to express genes from one organism in another? Let’s take a look at codon usage and...optimize codons for expression in a particular organism. Why is codon usage important? Translation and...for codon-usage of 257,468 genes across 8,792 organisms (Nakamura et al., 2000).  What is codon optimization...expression by changing synonymous codons based on an organism's codon bias. The idea is that scientists can make...mutations throughout a gene of interest based on an organisms codon usage bias to increase translational efficiency...

  12. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...across organisms. When using the MK system, many of the parts can be used in a variety of organisms, saving.... Crossref, https://doi.org/10.1038/s41598-019-46171-2. Resources on Addgene.org Modular Cloning (MoClo... these applications, no matter what your model organism is. MoClo background MoClo is a clever way to...MoClo kit recommendations for different modal organisms and applications.    Plant applications We...libraries and tools available for certain model organisms and backgrounds, MoClo can be invaluable for more... complex editing and screening experiments in organisms that don’t yet have these tools built out. While...many MoClo kits are designed with specific model organisms in mind, others are designed for flexibility. ...

  13. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...to mid-sized companies and organizations. Addgene as a nonprofit organization is very considerate and thoughtful... outside an organization The next step is to expand your efforts beyond your organization and/or scientific...people from various organizations in science and beyond. For example, I help organize scientific workshops...guide: Collaborating with others outside your organization also known as external collaborations. Interestingly...the ability to work with others outside one’s organization was found in the aforementioned survey as one... partnerships where we can leverage another organization’s expertise to our mutual benefit.  Our partnership...small: Collaboration opportunities within an organization but outside your comfort zone As part of my ...

  14. Typing CRISPR Systems

    Type
    Blog Post
    ...classification While CRISPR systems now seem neatly organized into their respective categories, arriving at ...CRISPR–Cas13. Nature, 550(7675), 280–284. https://doi.org/10.1038/nature24049 Altae-Tran, H., Kannan, S.,...terascale clustering. Science, 382(6673). https://doi.org/10.1126/science.adi1910 Chylinski, K., Makarova...Nucleic Acids Research, 42(10), 6091–6105. https://doi.org/10.1093/nar/gku241 Cong, L., Ran, F. A., Cox, D... systems. Science, 339(6121), 819–823. https://doi.org/10.1126/science.1231143 Cox, D. B. T., Gootenberg...-Cas13. Science, 358(6366), 1019–1027. https://doi.org/10.1126/science.aaq0180 Harrington, L. B., Burstein... enzymes. Science, 362(6416), 839–842. https://doi.org/10.1126/science.aav4294 Hu, C., Myers, M. T., Zhou...

  15. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...has taken place in other organisms. In many bacteria, unlike other organisms, CRISPR-induced double stranded...diseases have connections to either specific organisms or organism/gene interactions with the host. This scenario...can consider the microbiome as a collection of organisms, their genes, and metabolic processes, occupying...to diseases where the presence of just a single organism, such as Clostridium difficile, could be targeted... This approach successfully targeted specific organisms when using bacteriophages or mobile plasmids in...Pseudomonas species Expanding this tool to other organisms, the Ji lab brought genome and base editing to...genome engineering remains challenging for most organisms in the genus. Work from the Sun lab demonstrates...

  16. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ... ease of use and utility in a wide variety of organisms.  While much of current CRISPR research focuses... plant species, including commonly used model organisms like Arabidopsis and Medicago truncatula and several...universal functionality of the CRISPR system in most organisms, some plant-specific changes to CRISPR components...experimental design principles developed in other model organisms (see our CRISPR guide for common CRISPR principles...sequence) is consistent between plants and other organisms, but the promoter used to drive gRNA expression...your gene of interest into a target cell line or organism (Presented in figure 1). More information on Agrobacterium-mediated...CRISPR/Cas system. Plant Methods 9:39 . https://doi.org/10.1186/1746-4811-9-39 Gao Q, Xu W, Yan T, Fang...

  17. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...to one or a subset of organisms. Examples: “organism agnostic”, “model organisms”, “small genome”, “large...require the organism to be specified and are truly organism agnostic. Other tools require an organism to be ...only one, or a subset of organisms. In only a few cases the software is organism agnostic. Most tools are...tools will be marked as organism agnostic and have currently available organisms listed in this section...input types can be readily converted.  Examples: “organism”, “sequence”, “identifier”, and “load”. Trends... tools also requiring that the user specify an organism. Output diversity: Different tools provide results...large genome”, and “organism biased scoring”. Trends: Many tools can handle genomes of any size and are...

  18. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...Therapy: An Overview. Cells, 12(5), 1–41. https://doi.org/10.3390/cells12050785 Murlidharan, G., Samulski...Frontiers in Molecular Neuroscience, 7. https://doi.org/10.3389/fnmol.2014.00076 References and Resources...the Mouse Brain. PLOS ONE, 8(9), e76310. https://doi.org/10.1371/journal.pone.0076310 Damdindorj, L., Karnan...adeno-associated viral vectors. PLoS ONE, 9(8), 1–10. https://doi.org/10.1371/journal.pone.0106472 de Backer, M. W. A...Hypothalamus. Human Gene Therapy, 21(6), 673–682. https://doi.org/10.1089/hum.2009.169 Dudek, A. M., Pillay, S., ... Receptor. Journal of Virology, 92(7). https://doi.org/10.1128/jvi.02213-17 Favre, D., Blouin, V., Provost...Journal of Virology, 76(22), 11605–11611. https://doi.org/10.1128/JVI.76.22.11605-11611.2002 Griffin, J. ...

  19. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ... CRISPR-based genome editing in organoids By Ashley Waldron Organoids are three-dimensional cultures that...toolkit for performing genetics studies in organoids. The Organoid EasyTag System is a straightforward workflow...in an organoid model (Sun, et al. 2021).  Figure 1: Example schematic from the Organoid EasyTag...fetal-lung derived organoid system, the approach is intended to be amenable to organoids derived from other...find in this article: CRISPR-based editing in organoid Biosensors for detecting strigolactones The CRISPR... that mimic aspects of organ morphology and physiology in vitro and have emerged as a powerful model for...disease. In order to expand their model’s impact, organoid users have recently begun to develop CRISPR-based...

  20. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ... a wide range of organisms and cell lines. In C. elegans, one of the model organisms which I use to study...198:1347–1356 . https://doi.org/10.1534/genetics.114.170423 Schwartz ML, Jorgensen EM (2018) SapTrap vectors...methods – could greatly streamline editing in other organisms and systems, allowing rapid progress in a wide...Caenorhabditis elegans. Genetics 198:837–846 . https://doi.org/10.1534/genetics.114.169730 Bottcher R, Hollmann...cells. Nucleic Acids Research 42:e89–e89 . https://doi.org/10.1093/nar/gku289 Chen C, Fenk LA, de Bono M (... Nucleic Acids Research 41:e193–e193 . https://doi.org/10.1093/nar/gkt805 Dickinson DJ, Pani AM, Heppert...Selection Cassette. Genetics 200:1035–1049 . https://doi.org/10.1534/genetics.115.178335 Dokshin GA, Ghanta ...
Showing: 141 - 160 of 949 results