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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 322 results for: phy

Showing: 141 - 160 of 322 results

  1. Evolution of Lab Techniques

    Type
    Blog Post
    ...labeling it. The label can then be visualized by autoradiography and X-ray film for radioactively labeled probes...fragments of a DNA sequence of interest to create a physical map of the DNA. Starting in the 1970s, restriction...

  2. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...binds to endogenously expressed protein at physiologically relevant levels. Some vendors will also test...tissues that are known to express the protein at physiologically relevant levels (not overexpression systems...

  3. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ... refractory to enzymatic digestion, however, a physical fragmentation strategy is preferable. Nebulization...tank with a pressure regulator. Other methods for physical shearing exist as well, such as sonication and...

  5. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...without their problems, but much genetic and physiological data have been accumulated over the years using...Efficient Production of Gene-Modified Mice using Staphylococcus aureus Cas9. Sci Rep 6: . https://doi.org/10.1038...

  9. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...immunotherapy treatments. As treatments enter the clinic, physicians and researchers are learning much about how treatment... Within the tumor, this results in frequent physiological and morphological changes. Tumors differ in ...

  11. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...fixation-sensitive fluorochromes such as phycoerythrin (PE) and allophycocyanin. If assaying a secreted protein...

  13. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...precision with which signaling events occur in a physiological setting. Genetic techniques typically rely on...expensive peptides or small molecules and exceed a physiologically relevant exposure in intensity and/or duration...

  14. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ... Figure 3: Evolutionary tree depicting the phylogenetic relationship of common research species. Among...macaque, and on human brain slices cultured in physiological conditions (Chen et al., 2023, Chuapoco et al...

  18. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ...of Cas9's three-dimensional shape via x-ray crystallography, an advance they say should allow scientists...
Showing: 141 - 160 of 322 results