Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 758 results for: rest

Showing: 141 - 160 of 758 results

  1. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...promoter or regulatory regions of your gene of interest. If the plasmid that you choose does not also ...

  3. Plant Plasmids and Resources

    Type
    Collection
    ...can be used as empty backbones for your gene of interest for expression in plants. You can search the table...

  4. TALEN Guide

    Type
    Collection
    ...genome are every 500 bp. TAL effectors have some restrictions (for example, the target must start with a T...

  5. Viral Vectors

    Type
    Collection
    ...instead encode for a specific gene or other research interest. To produce viruses with alternate (non-virus-...

  7. TALEN Plasmids and Kits

    Type
    Collection
    ...cell lines. Features include (i) Esp3I (BsmBI) restriction sites for full compatibility with the Golden ...

  8. CRISPR References and Information

    Type
    Collection
    ...reads from PCR amplicons that cover your locus of interest and correspond to edited and non-edited cell populations...

  9. Caltech Systemic Capsids

    Type
    Collection
    ...method, mouse strain, target cell type or tissue of interest, and model organism under study. *Many engineered...

  12. Evolution of Lab Techniques

    Type
    Blog Post
    ...a sequence recognized by the restriction enzyme MstII. When a restriction digest with MstII and southern...than real-time PCR (4). Restriction mapping and modern sequencing Restriction mapping informs on DNA sequence...different restriction enzyme digests. Incubating a DNA sample of unknown sequence with restriction enzymes...sequence of interest to create a physical map of the DNA. Starting in the 1970s, restriction mapping was...has changed, restriction enzymes are certainly not dead yet! Labs still keep restriction enzymes on hand...three historical techniques: southern blotting, restriction mapping, and sequencing gels, as well as their... (2). Southern blotting, in conjunction with restriction digest analysis, is used to identify the presence...

  13. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...Method C: Restriction cloning of a restriction enzyme fragment containing the DNA of interest and a attL-entry...scientists are no longer limited to traditional restriction enzyme cloning. Instead, you can choose a molecular...vectors, one can use Gateway to clone a gene of interest into a variety of expression systems. Keep reading...generates the entry clone containing the DNA of interest flanked by attL sites. As a byproduct of the reaction... a result, an expression clone with the DNA of interest flanked by attB sites is generated. As in the ...recombination, and it is exchanged with the gene of interest during the BP or LR reactions. Since the CcdB ...Figure 5: Method C to create an entry clone: Restriction cloning the inset into an attL-containing entry...

  14. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...the mutation of interest. Gel electrophoresis diagrams of the anticipated restriction analysis results... by Restriction Fragment Length Polymorphism (RFLP) analysis (right). Introduction of restriction site...conventional cloning (to introduce or remove restriction sites), in mapping of regulatory elements (to...the plasmid template (blue), and introduce a restriction site “A*” (in addition to the “A” site already...in the parent vector). Also shown are the “B” restriction sites, which flank the targeted region. Upon ...amplification, the template is removed by DpnI restriction digestion leaving behind only the mutagenized...primers to successfully anneal to the plasmid of interest during the PCR reaction. Ideally, your primers...

  15. Common Injection Routes in Mice

    Type
    Blog Post
    ...institutional oversight is required. Restraint: mechanical restraint or anesthesia Location: tail vein or...you might after getting a vaccine. Restraint: mechanical restraint Location: thigh Gauge: 25–27 Volume... create xenograft models. Restraint: back scruffing or mechanical restraint Location: back or hind legs...Injection Route Restraint Location Gauge Volume Skill Intravenous Mechanical restrainer or anesthesia ...potential pain or discomfort of the mice, and restraint method. Many different substances can be injected... potential off-target pokes to nearby organs. Restraint: scruffing Location: peritoneal cavity Gauge: ..., like on the back. Depending on the site and restraint method, subcutaneous injections take some practice...

  16. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...selectively detecting the target-of-interest in your assay and sample-of-interest. The approaches available broadly...antibody can detect the antigen of interest in the protocol of interest, ideally at endogenously expressed...a candidate antibody can detect the antigen of interest in the given protocol. This requires use of one...ability of an antibody to detect the antigen of interest when expressed at endogenous levels (Laflamme ... a robust level, similar to that in samples of interest. If you are working with cells that are amenable...approach to antibody validation. If your protein of interest is essential to the cell’s function, it likely...on the specific antibody:target combination of interest (Figure 1). It can therefore be helpful to try...

  17. To Each HIS Own

    Type
    Blog Post
    ... containing the protein of interest flowed through. The protein of interest binds to the metal ions, becoming... of interest. A high imidazole elution buffer disrupts the interaction of the protein of interest and ...the N- or C-terminus of a recombinant protein of interest. The tag was developed as a means to purify a ...proteins and other contaminants. The protein of interest is then eluted using a high imidazole buffer that...outcompetes the interaction between the protein of interest and the metal ions.      Fig. 1: A sample...sample containing a polyhistidine tagged protein of interest (purple) and several contaminating proteins (blue...through the Ni2+-containing resin. The protein of interest (purple) binds to the Ni2+ and is immobilized ...

  18. MXS Chaining

    Type
    Blog Post
    ...principle behind MXS-chaining is repeatable, restriction-enzyme based (chaining-based) cloning. The modules...Modules are assembled one at a time by standard restriction enzyme digestion followed by ligation. Upon ligation...the repeatability of the method. Note that the restriction sites of the MCS are only regenerated at the ... 3’ termini of the ligation product, and the restriction site between the modules is eliminated upon ligation... using these techniques are free of specific restriction enzyme recognition sites. Thus, chaining methods...Technology Pros Cons References Golden Gate Type II restriction enzymes Can assemble up to 10 modules in a single...reaction, can assemble large DNA sequences, no restriction enzymes needed Not optimal for joining sequences...

  19. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ... am talking about making sure the audience is interested, educated and talking as a result of the content...where the questions are obvious, the answers uninteresting, the panel members all say the same thing and... a well-planned approach to making the event interesting and engaging. Long before the event... Choose... should be taking good notes and discovering interesting stories, riffs or opinions that each panelist...moderator should identify the funny, provocative or interesting stories that will make for a fun discussion. ...questions in a directed way to bring out these interesting stories and remind the panelists of the stories...spontaneity, but having a good handle on some interesting questions and topics in advance is key. Make ...

  20. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...scientific interests were or what I wanted to do with myself when I was 18. I just knew I was interested in trying...some perspective and advice to any undergraduate interested in getting a taste of the role of a scientist...begin. Here’s my advice: Reflect on your personal interests While it’s perfectly fine to be unsure, it helps...excited about the science allows you to ask more interesting questions and your excitement will in turn inspire...makes for an experience more tailored to personal interests. Talk to people, ask questions, and dig deep Talk...though, try to be conscious of who has your best interests at heart. A great piece of advice I’ve gotten ...who have taken career paths that you would be interested in can also help you clarify your goals and define...
Showing: 141 - 160 of 758 results