We narrowed to 1,054 results for: tra

...mAb Collection . Once the plasmids are transiently transfected into mammalian tissue culture cells, the...is present in a sample — just to name a few. Traditional sources of antibodies for research purposes include...single domain antibodies for the conformational trapping of membrane proteins. Elife. 2018 May 24;7. ...

... mTORC1 Complex Symbol Name 4E-BP Eukaryotic translation initiation factor 4E, binding protein 1 Cyclin...Cyclin D1 Also known as CCND1 eIF-4E Eukaryotic translation initiation factor 4E GBL/mLST8 MTOR associated...Neurofibromin 1 PRAS40 Also known as AKT1S1; AKT1 substrate 1 (proline rich) PTEN Phosphatase and tensin homolog....2014.01.001. PubMed PMID: 24508508 . Targeting the translation machinery in cancer. Bhat M, Robichaud N, Hulea...

...plasmids is a bacterial stab (of a laboratory E. coli strain). Addgene continues to monitor its library and ... Integrative vector assembly of up to three transcription units in the oleaginous yeast Y. lipolytica ...Restoration of prototrophy in common S. cerevisiae lab strains - Ralser Lab Return to top Resources for... in bacterial genome editing is not always straightforward. Read our CRISPR Methods for Bacterial Genome...

... used to generate double-stranded breaks ( DSBs or cuts ) or single-stranded breaks ( nicks ). ID Name...Cas9 (dCas9) can be fused to a transactivator and used as a transcriptional activator . ID Name Insert Antibiotic...

...this serotype to deliver Cre-dependent transgenes in Cre transgenic lines. As part of our standard viral...serotype permits robust retrograde access and transgene delivery to projection neurons. Specific classes...titering by SYBR green qPCR , confirmation of the transfer plasmid with PCR, purity assessment by silver ...

...plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs)....Genome Editing Cut Base Edit Nick Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA...nuclease. When FokI dimerizes, it generates a double-strand break (DSB) at a specific sequence. Two unique ...

...CRISPR plasmids for transcriptional repression of target genes....Catalytically dead Cas9 (dCas9), or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down...down gene expression by interfering with transcription. This interference is most efficient when the gRNA...

...technical reports from the research community demonstrating AAV performance under various experimental conditions... vectors from the Penn Vector Core have been transferred to Addgene for distribution. Look up the IDs ...actual material being referenced in the Material Transfer Agreement (MTA) and its inclusion is what gives...

...reasons: Transfer plasmids with sequence from the 5′ to the 3′ ITR exceeding 4.7 kb Transfer plasmids ... Terms of Purchase and the applicable material transfer agreements (MTAs) and limited use label licenses...

... other difficult-to-target antigens that are intractable via animal immunization. IPI is a nonprofit research...researchers with powerful tools to investigate neurotransmission, synapse formation, neural connectivity, plasticity... top Integrins Integrins are a family of mechanotransduction receptors, comprised of alpha-beta subunit...

...listing of transfer status for viral preps.... Viral Vector Packaging Service Penn Vector Core Transfer Viral ...Viral Vector Packaging Service: Penn Vector Core Transfer The University of Pennsylvania Vector Core (Penn...inventory , which includes all viral vectors transferred from the Penn Vector Core. Chemogenetics (including...

..., that will probably never happen. Yet with transcription activator-like (TAL) effectors, scientists in... the plant bacterial pathogen Xanthomonas. The central TAL targeting domain is composed of 33-35 amino...sequence-specific TAL effectors for modulating mammalian transcription. Nat Biotechnol. 2011 Feb;29(2):149-53. PMID...

...Ettema, T. J. (2016). Tracing the archaeal origins of Eukaryotic Membrane-Trafficking System building blocks...known for their roles as regulators of membrane traffic, they also play critical roles at cilia, centrosomes...

...specimens. These technologies have the potential to transform neuroscience research by bringing the precision...enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells...interneurons. For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent...

...using various delivery methods including intracerebroventricular (ICV) delivery in newborns, and retro-orbital...Tasic, B. (2025). A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell.... A., Larsen, R., Gore, B. B., Szelenyi, E. R., Trader, C., . . . Tasic, B. (2021). Enhancer viruses for...

...for recommended applications. Addgene values transparency and accessibility in research and therefore ...our production process? At Addgene, we provide transparency in our production process by sharing...

...KLFs) are a family of at least 17 DNA binding transcriptional regulators with diverse roles in processes ...elements or GC-rich regions on DNA to mediate transcriptional activation and/or repression. Continuing collaborative...

...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...

...different cell types. Zinc finger arrays can be transferred in-frame to these vectors....either OPEN or modular assembly can be easily transferred in-frame to any of these vectors using unique...

...currently available from our catalog. Addgene values transparency and accessibility in research. For each antibody... other difficult-to-target antigens that are intractable via animal immunization. Addgene's antibody catalog...