We narrowed to 482 results for: vent
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TypeGuide...RuvC and HNH, can be altered to reduce cleavage events to a single cut (nick) or no cuts. Cas9 nickase...the donor template, incorporating mutations that prevent further Cas9 cleavage will improve the accuracy...and NGS (other genomic regions). Compared to conventional methods for genomic purification, CRISPR-based...Rekhtman, N., De Stanchina, E., Lowe, S. W., & Ventura, A. (2014). In vivo engineering of oncogenic chromosomal...
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The Twelve Days of Antibodies
TypeBlog Post... to me… A recombinant Anti-PSD-95! 🎶On the seventh day of Antibody, Addgene gave to me… Antibodies...gave to me… A recombinant Anti-APP! 🎶On the eleventh day of Antibody, Addgene gave to me… A resource... -
The 12 Days of CRISPR: 2021
TypeBlog Post...PRIME-del (Precise genomic deletions). On the seventh day of CRISPR, Addgene gave to me: programmed genome...gave to me: a primer on prime editing. On the eleventh day of CRISPR, Addgene gave to me: a Cas9 nickase... -
The Twelve Days of CRISPR
TypeBlog Post...generation (and dual base editors, too!). On the seventh day of CRISPR, Addgene gave to me: CRISPR methods...promoter-enhancer 3D chromatin structures using CRISPR. On the eleventh day of CRISPR, Addgene gave to me: methods for... -
Bringing Renewable Energy to Addgene
TypeBlog Post...-20C freezer temperatures, biosafety cabinet ventilation, and lab lighting/HVAC requires a significant...multiple streams for composting and recycling in the event the object in question cannot be washed or reused... -
Congratulations, Deck The Lab winners!
TypeBlog Post...second place, Dr. Anne Madden created a microbe advent tree displaying over 25 archaea, bacteria, fungi...viruses. Final tree with the complete #MicrobeAdventCalendar ornament set (plus more!) It features archaea... -
Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6
TypeBlog Post...therapeutics. After a brief stint at the venture capital firm, Flagship Ventures, Koeris helped change Novophage's... -
Fueled by Coffee at #SfN14
TypeBlog Post...coffee would be consumed throughout the entire 5 day event. Though, to be fair, I think this is a conservative... an inspiring, thought-provoking, collaborative event – all while keeping the party going! At Addgene,... -
Making Career Development an Integral Part of the Science Training System
TypeBlog Post...which, in the absence of support and structure, prevent science from attracting and keeping diverse talent...us for the pd|hub Workshop Report Public Release Event scheduled for March 4th from 2:30-4:00 pm ET. You... -
Q&A With Julie Cicalese, the Newest Member of the Addgene Board of Directors
TypeBlog Post... role in organizing an Art in Giving think tank event where we brought a multidisciplinary group of scientists...Art in Giving was able to fund. Organizing that event and seeing the outcome was wonderful! I also volunteer... -
Why Do I Need an MTA Anyway?
TypeBlog Post...Rights to all research results, modifications, and inventions created by the Recipient Right to file patent...patent applications on modifications or inventions Right to publish without editorial comment or review... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog Post...collaboration with the Porteus lab [2]. With the advent of CRISPR/Cas9, we began to explore the paired ...This method allows for deletion screening by conventional PCR. Biallelically disrupted cells can be easily... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog Post...inspiring. I was especially excited to attend this event because Jennifer did her graduate work one floor...importance of this type of experimentation to the eventual translation to the clinic and cures. There is ... -
Antibodies 101: Fc Effector Functions
TypeBlog Post...Antibodies can bind to a pathogen and physically prevent it from entering a cell, a non-Fc-dependent effector...classical complement pathway, starting a cascade of events that results in the pores being opened in the pathogen... -
Year of the Camelid: Antibody Style
TypeBlog Post...refresher, some of the advantages of sdAbs over conventional antibodies include: Excellent tissue penetration..., RANbodies can be used as an alternative to conventional primary antibodies, and there are unique benefits... -
History of CRISPR Cas - A tale of survival and evolution
TypeBlog Post...host cell. A few virus particles have learned to circumvent the CRISPR/Cas defense by generating a single...single point mutation in the PAM sequence, preventing the Cas nucleases from re-identifying it (3). Surprisingly... -
Summer SciComm: Science Storytelling
TypeBlog Post...why your experiment has not worked…again…for the seventh time…. I find that once I sit down and talk to...mean? How did the researchers solve the problem preventing them from moving forward? Answering these questions... -
Summer SciComm Series: Master of SciComm
TypeBlog Post...such that a desire to once again write for fun eventually saw an English minor tacked onto my BSc. With...level of career satisfaction indeed. For many venturing forth from tertiary study, the myriad trajectories... -
Identifying Sequence Elements with SnapGene's Feature Database
TypeBlog Post...visualizing, and documenting their procedures. Preventable errors in the design of cloning strategies set...related versions that have different properties. To prevent misidentification, we augment the database with... -
New and Upcoming Viral Vectors - Spring 2019
TypeBlog Post...service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of...far in 2019. You can also browse our entire AAV inventory! Our new AAVs include: pGP-AAV-syn-FLEX-jGCaMP7b-WPRE...