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We narrowed to 482 results for: vent

Showing: 141 - 160 of 482 results
  1. CRISPR Guide

    Type
    Guide
    ...RuvC and HNH, can be altered to reduce cleavage events to a single cut (nick) or no cuts. Cas9 nickase...the donor template, incorporating mutations that prevent further Cas9 cleavage will improve the accuracy...and NGS (other genomic regions). Compared to conventional methods for genomic purification, CRISPR-based...Rekhtman, N., De Stanchina, E., Lowe, S. W., & Ventura, A. (2014). In vivo engineering of oncogenic chromosomal...
  2. The Twelve Days of Antibodies

    Type
    Blog Post
    ... to me… A recombinant Anti-PSD-95!    🎶On the seventh day of Antibody, Addgene gave to me… Antibodies...gave to me…  A recombinant Anti-APP!   🎶On the eleventh day of Antibody, Addgene gave to me…  A resource...
  3. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...PRIME-del (Precise genomic deletions).  On the seventh day of CRISPR, Addgene gave to me: programmed genome...gave to me: a primer on prime editing.  On the eleventh day of CRISPR, Addgene gave to me: a Cas9 nickase...
  4. The Twelve Days of CRISPR

    Type
    Blog Post
    ...generation (and dual base editors, too!).  On the seventh day of CRISPR, Addgene gave to me: CRISPR methods...promoter-enhancer 3D chromatin structures using CRISPR. On the eleventh day of CRISPR, Addgene gave to me: methods for...
  5. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    ...-20C freezer temperatures, biosafety cabinet ventilation, and lab lighting/HVAC requires a significant...multiple streams for composting and recycling in the event the object in question cannot be washed or reused...
  6. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ...second place, Dr. Anne Madden created a microbe advent tree displaying over 25 archaea, bacteria, fungi...viruses. Final tree with the complete #MicrobeAdventCalendar ornament set (plus more!) It features archaea...
  7. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...coffee would be consumed throughout the entire 5 day event. Though, to be fair, I think this is a conservative... an inspiring, thought-provoking, collaborative event – all while keeping the party going! At Addgene,...
  8. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ...Rights to all research results, modifications, and inventions created by the Recipient Right to file patent...patent applications on modifications or inventions Right to publish without editorial comment or review...
  9. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...Antibodies can bind to a pathogen and physically prevent it from entering a cell, a non-Fc-dependent effector...classical complement pathway, starting a cascade of events that results in the pores being opened in the pathogen...
  10. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...refresher, some of the advantages of sdAbs over conventional antibodies include:  Excellent tissue penetration..., RANbodies can be used as an alternative to conventional primary antibodies, and there are unique benefits...
  11. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...host cell. A few virus particles have learned to circumvent the CRISPR/Cas defense by generating a single...single point mutation in the PAM sequence, preventing the Cas nucleases from re-identifying it (3). Surprisingly...
  12. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ...why your experiment has not worked…again…for the seventh time….   I find that once I sit down and talk to...mean? How did the researchers solve the problem preventing them from moving forward? Answering these questions...
  13. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ...such that a desire to once again write for fun eventually saw an English minor tacked onto my BSc. With...level of career satisfaction indeed.   For many venturing forth from tertiary study, the myriad trajectories...
  14. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    ...service in 2016, we’ve added many new tools to our inventory of ready-to-use viral vectors. Here are some of...far in 2019. You can also browse our entire AAV inventory! Our new AAVs include: pGP-AAV-syn-FLEX-jGCaMP7b-WPRE...
Showing: 141 - 160 of 482 results