We narrowed to 402 results for: CAPS;

1. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

   Type

   Blog Post

   ...lab at Stanford develops engineered DNA systems capable of storing data and computing inside living cells...

2. Antibodies 101: Producing Recombinant Antibodies

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   Blog Post

   ...PO, Eriksson K, Shokri A, Chotteau V. Antibody capture process based on magnetic beads from very high ...

3. Course-Based Undergraduate Research Experiences (CUREs)

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   Blog Post

   ...increased students’ confidence in their research capabilities and laboratory and critical thinking skills ...

4. Uncovering Ancestral Bias in CRISPR Research

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   Blog Post

   ...and Fultineer analyzed the gnomAD dataset, which captures a broader swath of human genetic diversity, to...

5. MXS Chaining

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   Blog Post

   ...precision cloning method with high throughput capability. PloS one. 2008;3(11):e3647 doi: 10.1371/journal.pone...

6. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   Blog Post

   ...CRISPRko (Brie), CRISPRi (Dolomiti), and CRISRPa (Caprano) libraries for the mouse genome! Other Resources...

7. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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   Blog Post

   ...gRNA, to your cells of interest. Cas9 RNPs are capable of cleaving genomic targets with similar efficiency...

8. A Guide to Starting Your Own Journal Club

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   Blog Post

   ...available to those who were unable to attend. We capture the desktop and audio recordings of the meeting...

9. Droplet Digital PCR for AAV Quantitation

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   Blog Post

   ...containing thousands of droplets) can be sufficient to capture the information needed for your PCR experiment....

10. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    Blog Post

    ... your target cell type Serotype: Choose the AAV capsid or lentiviral prep needed Tags: Identify a specific...

11. Transferable Skills Guide: Identifying Your Transferable Skills

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    Blog Post

    ...when balancing a handful of demanding courses, a capstone paper I really wanted to hit out of the park, ...

12. Negative Can Be Positive: Open AAV Data with Addgene

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    Blog Post

    ...By providing many of the details not generally captured in the literature, scientists can use the Data...

13. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type

    Blog Post

    ... an electrical jolt and cells become perfectly capable of synthesizing fluorophore fusions on their own...

14. Tips for Screening with Yeast Two Hybrid Systems

    Type

    Blog Post

    .... Obrdlik, P., El-Bakkoury, M., Hamacher, T., Cappellaro, C., Vilarino, C., Fleischer, C., Ellerbrok, ...

15. Cpf1: A New Tool for CRISPR Genome Editing

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    Blog Post

    ...counterparts, they will also be easier to deliver in low-capacity vectors, such as adeno-associated viral (AAV) ...

16. The Importance of a Fun Workplace: Company Culture at Addgene

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    Blog Post

    ... ordinary environment Team building events like Escape the Room or Habitat for Humanity take you and your...

17. A Career in Grants: How To Become a Grant Professional

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    Blog Post

    ...application and submitting it, to evaluating the capacity of an organization to receive and manage funds...

18. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post

    ...stability, GFP can be used in lineage tracking capacities in cell fate studies. It can also be used, when...

19. Your Lentiviral Plasmid FAQs Answered

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    Blog Post

    ...meaning they can be used to create virus that is capable of infecting target cells, but cannot produce any...

20. Negotiating Work and Life: How to Find the Joy

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    Blog Post

    ... help. Your partner is equally responsible and capable. The only exception is that men cannot actually...

21. CRISPR 101: Any Base Transversion Editors

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    Blog Post

    ...mutagenesis, the team progressed to gGBEv6.3, which was capable of 80% efficient G → Y editing. Like other transversion...

22. Twenty Years of Sharing: Expanding Accessibility

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    Blog Post

    ...smaller, systemic changes, like using alt-text, captions, and inclusive design in our digital content. ...

23. Plasmids 101: Broad Host Range Plasmids

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    Blog Post

    ...leguminosarum, R. meliloti, Rhodobacter sphaeroides, R. capsulatus, Rhodopseudomonas spheroides, Salmonella spp....

24. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

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    Blog Post

    ...single cell screening to isolate a clonal cell line capable of producing ultrasound contrast, and gene silencing...

25. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    Blog Post

    ...in a “nuclease dead” Cas13a (dCas13a), that is capable of binding target RNA but lacks the ability to ...

26. Fluorescent Proteins 101: Aptamer Fluorophores

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    Blog Post

    ...dimerized Broccoli to further enhance its biosensor capabilities (see Fig. 1b). The future of aptamer fluorophores...

27. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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    ...above). The VQR, EQR, and VRER Cas9 variants are capable of cleaving genomic DNA in mammalian cells and ...

28. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    Blog Post

    ...these eGFP reporters to analyze the base editing capability of the seven human APOBEC3 proteins, finding ...

29. An Inside Look into Addgene's Dev Team

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    Blog Post

    ...to for each sprint, to ensure we will have the capacity to finish all of the work. Since we work in iterative...

30. New Tools Enable CRISPRa for Neuroscience Applications

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    Blog Post

    ...CNS by lentivirus, which has a larger packaging capacity than AAV. Such an approach was described recently...

31. CRISPR-mediated Plant Base Editors

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    ...Yang. "Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system." ...

32. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

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    Blog Post

    ...reprogramming (or “Yamanaka”) factors, they changed the landscape of regenerative medicine. Their work opened up...

33. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    Blog Post

    ... dCas9-VP64 fusion and a modified gRNA that is capable of interacting with an additional RNA-binding transcriptional...

34. Mesothelioma - Causes, Symptoms, and Treatment

    Type

    Blog Post

    ... i.e., any exposure to asbestos is potentially capable of causing mesothelioma many years later. While...

35. Important Considerations in Optogenetics Behavioral Experiments

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    Blog Post

    ... usually controlled automatically using motion capture software such as EthoVision. Consult the literature...

36. Data Freedom: The Expansion of Data Sharing in Research Publications

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    ...sizes) but images should be unprocessed and in capture format. This raises another issue of proprietary...

37. Antibodies 101: Beyond Surface Labeling

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    Blog Post

    ...Proliferation dyes can visualize proliferative capacity and speed of cells. (a) With each division half...

38. Antibodies 101: Introduction to Gating in Flow Cytometry

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    Blog Post

    ...principles of gating in flow cytometry. Let’s briefly recapitulate what we’ve covered in this blog post. First,...

39. CRISPR 101: Homology Directed Repair

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    Blog Post

    ...that form when elongation of the invasive strand “captures” and synthesizes DNA from the second DSB end. ...

40. Hot Biosensors 2022: Year-End Roundup

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    Blog Post

    ...excitable under blue light (470-495 nm) and is capable of local temperature sensing with sub-millisecond...