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We narrowed to 728 results for: SON

Showing: 321 - 360 of 728 results

  1. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    ...commonly used for recombineering can be found in Thomason et al and Sharan et al (a few strains can also...ecosalplus. PubMed PMID: 27223821. 4. Sharan SK, Thomason LC, Kuznetsov SG, Court DL. Recombineering: A ...19180090. PubMed Central PMCID: PMC2790811. 5. Thomason, L. C., Sawitzke, J. A., Li, X., Costantino, N...

  2. Typing CRISPR Systems

    Type
    Blog Post
    ...P. C., Shmakov, S. A., Silva, C., Tolstoy, I., Johnson, D. B., Koonin, E. V., & Quatrini, R. (2021). Evolution...https://doi.org/10.1093/nar/gkz1197 Samai, P., Pyenson, N., Jiang, W., Goldberg, G. W., Hatoum-Aslan, ... Hallmark, T., Keiser, D., Bondy-Denomy, J., & Jackson, R. N. (2021). Positioning diverse Type IV structures...

  3. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ... is FRET and what's it good for? Fluorescence resonance energy transfer (FRET) is a biophysical interaction... excited, it can transfer energy directly via resonance to the second fluorophore (the acceptor) if the...PMID: 17512008. 4. Fessenden, James D. "Förster resonance energy transfer measurements of ryanodine receptor...

  4. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...heteroduplex transitions back to DNA duplex, and it’s the reason guide sequences have a maximum length of 20 bases...Sternberg et al., 2015).     Figure 8: Comparison of Cas9:RNA crystal structure (left, PDB ID 4ZT0..., Dangerfield, T. L., Jung, K., McCool, R. S., Johnson, K. A., & Taylor, D. W. (2022). Structural basis...

  5. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...for cloning but PX330 only uses 20 nt? A16: The reason for the 30 nt region on the upper is because the...doi.org/10.1126/science.1231143  Elliott, B., Richardson, C., Winderbaum, J., Nickoloff, J. A., & Jasin...cells differing exclusively at two early onset Parkinson Point mutations. Cell, 146(2), 318–331. https:...

  6. Antibody Guide

    Type
    Guide
    ...using sonication to break DNA up into fragments of 300-1000 bps in length. Note: This sonication process...may need additional processing steps, such as sonication. Denature proteins, using heat and/or chemicals...housekeeping genes). This allows for relative comparison of expression between different samples, by normalizing...normalizing protein expression to the controls before comparison. Alternatively, the samples can be normalized...ChIP protocols use enzyme digestion instead of sonication. This approach is gentler but results in non-...isolated and analyzed. Validation relies on size comparison of peptides. Independent antibody validation ...

  7. Chemogenetics Guide

    Type
    Guide
    ...19607790 (Link opens in a new window) Atasoy D, Sternson SM (2018). Chemogenetic Tools for Causal Cellular...Magnus CJ, Lee PH, Atasoy D, Su HH, Looger LL, and Sternson SM (2011). Chemical and genetic engineering of...Ramirez MH, Hu X, Galvan A, Basu J, Michaelides M, Sternson SM (2019). Ultrapotent chemogenetics for research...6. PMID: 11402033 (Link opens in a new window) Sternson SM, Roth BL (2014). Chemogenetic tools to interrogate...26399324 (Link opens in a new window) Vardy E, Robinson JE, Li C, Olsen RHJ, DiBerto JF, et al. (2015)... Xiong M, Tao Y, Gao Q, Feng B, Yan W, Zhou Y, Kotsonis TA, Yuan T, You Z, Wu Z, Xi J, Haberman A, Graham...

  9. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    ... research, for decades. Its short life cycle, reasonably close genetic homology to humans, and small size... Rachel Leeson...

  10. CRISPR 101 eBook...en español

    Type
    Blog Post
    ...repositorio. Este increíble regalo permitirá que más personas accedan a recursos útiles para sus experimentos... Rachel Leeson...

  12. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    ... of holiday spirit still linger (despite the unseasonably warm weather we had last weekend). We’ve spent...DeckTheLab. Getting in the spirit for the holiday season. Santa frog is coming to town. @Addgene @UMich_...

  13. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ...today✂️❄️🦠thanks to the awesome templates by @CVRHutchinson @CVRinfo. Check them out: https://t.co/uDEE9PsspD...  Rachel Leeson...

  14. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    ...Addgene. A team of undergraduate students led by Dr. Song Tan at Penn State developed the plasmids, pPSU1 ...1000 Lanes Cost $200-$500  <$10 $20-30 Cost Comparison: While convenient, commercial ladders cost between...

  16. Summer Fun at Addgene!

    Type
    Blog Post
    ...Here at Addgene, we’re always inspired by the new seasons, so we've made some big summer plans this year!...                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      Rachel Leeson...

  17. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    ... to #DeckTheLab with virus snowflakes from @CVRHutchinson #SciArt #STEAM #WinterWonderlab @Addgene Make...We hope everyone has a safe and festive Holiday season. 🙌 🥂 🎄 🎉 😷 Remember wear a mask,↔️social distance...

  18. Editor's Choice, July 2016

    Type
    Blog Post
    ...easier to generate mouse models.   Cpf1 Update: Comparison to Cas9 and NgAgo As you might expect given the...Generating Mouse Models Using CRISPR/Cas9 Cpf1 Update: Comparison to Cas9 and NgAgo Special Delivery: Fluorophore...

  20. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    ...introduction to plasmids with Director of Biology Lianna Swanson was very popular, with the students excited to ...different career options in science, with Abigail Anderson commenting that “There is not just a scientist...

  22. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ... protein that binds an alternate sequence. By comparison, Abil explained, the specificity of other RNA...less reliable results. She also notes that for reasons that are not yet entirely clear some PUF proteins...

  23. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ...honest feedback to the manager for a variety of reasons. They may feel that giving feedback will be perceived... to be too busy to be receptive.   Whatever the reason, it is a fortunate and successful manager that ...

  27. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...advantageous. This new technique, developed in Thoru Pederson’s lab, brings us one step closer to mapping the...Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. Proc Natl Acad Sci U S A. 2015 Mar 10;112(10...

  29. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...follow their passions, be happy, and match their personal goals to their career choices.” She points out...hard work, collaboration, and staying honest with personal goals. Stay tuned for the follow up blogs on "...

  30. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    ...Verkhusha popping up again and again, and for good reason. We all know scientists have used fluorescent proteins... higher resolution images in vivo. “One of the reasons we can’t localize one cell at a depth of one centimeter...

  31. Adenovirus Guide

    Type
    Guide
    ... cloning methods, including restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may... Bullard, B. L., Corder, B. N., Gordon, D. N., Pierson, T. C., & Weaver, E. A. (2020). Characterization...Asiedu, C., Geisbert, J., Stanley, D., Honko, A., Johnson, J., Mulangu, S., Pau, M. G., Custers, J., Vellinga...gene and cell-based therapies in the new era of personalized medicine . Genes & Diseases, 4 (2), 43–63. https...window) Luo, J., Deng, Z. L., Luo, X., Tang, N., Song, W. X., Chen, J., Sharff, K. A., Luu, H. H., Haydon...

  32. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...experimental problems, legal restrictions, or any other reason. But this way, as soon as we become aware the related...toast to the new paper (or after — hey, another reason to celebrate!), send us a quick email at deposit...

  33. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ... with multiple serotypes pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] AAV1 Optogenetics Edward Boyden New ...resources!   This blog post was written by Rachel Leeson and Brian O'Neill.  More resources on the Addgene...

  34. An Addgene Summer Internship

    Type
    Blog Post
    ...Covid gene, a toxin, or an oncogene, for safety reasons, and the insert size should be a max of 500 bp ...biochemistry in college and play the drum set as their personal instrument. They are the oldest sibling in a Guatemalan...

  36. Plasmids 101: Gene, enhancer, and promoter trapping

    Type
    Blog Post
    ...monitored genes and mutants - viral integration, transposon, and many more! Check out Addgene’s catalog to...resources on the Addgene blog Plasmids 101: Using Transposons in the Lab Plasmids 101: Screens vs. Selections...

  37. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ... we love camelids too, though not just for the reasons the UN describes. In honor of the Camelid family...Arbabi-Ghahroudi, 2017)!      Figure 1: Comparison of a heavy-chain only antibody and single-domain...
Showing: 321 - 360 of 728 results