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We narrowed to 1,106 results for: Ide

Showing: 161 - 180 of 1106 results

  1. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    ...database of common features, and devising rules for identifying a feature even when the match is imperfect. SnapGene's...catalog every variant of a feature. Instead, we identified common variants, and then crafted a detection...reasonable rule is to require at least 96% sequence identity when detecting a reference feature. For a coding...match even when the DNA sequence has changed. Identifying plasmid control elements Coding sequence features...original literature, some of it decades old, to provide reliable feature annotations. Even with these extensive...that have different properties. To prevent misidentification, we augment the database with new fluorescent...protein variants. The future of DNA feature identification What’s next for common features? We plan to...

  2. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ... it! Further Reading and Outside Resources http://www.businessinsider.com/how-to-get-the-most-out-of-job-fairs...your lab, right? Wrong! While it is clearly a good idea to be diligent in your research, remember that you...themselves, resources are often scarce and difficult to identify. So what specifically should you do? What follows... elaborated on later. As for what job fairs to consider, some of the best options are the Naturejobs Career...most relevant experience at the top. You may also consider visiting any of the employer’s career pages to... apply for the position. This post on Business Insider nicely summarizes good job fair practice. Career...and other online career resources Once you’ve identified places you’d like to work, get online and take...

  3. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...I led. It’s divided into the key takeaway themes and customer pain points we’ve identified. I can’t wait...software product management, I confess that I had no idea what software product management was either. Coming...

  4. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    ...majority of cells failing to develop the desired identity. The development of single cell technologies, ... RNA-seq (scRNA-seq), now allows scientists to identify the gene expression patterns unique to the few...Morris Lab at Washington University in St. Louis provides a combinatorial indexing strategy that simultaneously...simultaneously tracks clonal history and cell identity at the single-cell level, thus linking ancestral gene...Biddy et al., 2018). Tracking lineage and cell identity at the single-cell level with CellTag CellTagging... region. The variable region acts as a unique identifier for individual cells. Each cell is labeled with.... The fixed region acts as a library-specific identifier and allows cells to be tagged with different ...

  5. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...2) creating a pictorial guide for our engineers (Fig. 2). Creating this guide was relatively easy. I took...different interpretations of what we discussed and decided. I needed to a) figure out the sources of miscommunication...non-scientist friends and family is going to vary widely. The way you communicate your science at a poster...collaborations with other scientists within and outside your field, and any number of interactions with...the context you do, which means you’ll need to provide that context yourself. Simple words are not so...the same question and asking can help the team identify possible points of confusion in the future. Improving...

  6. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...journal club. Sorting out the logistics Once you decide on the topic, there are a number of administrative...notice for the first meeting so that people can set aside time in their schedules and read the paper (PS: ... on single papers could be more important.  Any slides, presentations, recordings, and other materials... ensure that the journal club is useful for the widest audience, you should get feedback from those who...everyone to offer thoughts you may not have even considered. These questions should be tailored to the group...priority for the participants. Perhaps people feel confident in their own knowledge of the field. Perhaps interest... not actively participating, it may be time to consider ending the meeting. This is a common failing in...

  7. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...developers of many of these probes, we decided to write this quick guide to help researchers choose the probe...supplied (Baloban et al., 2017).  It is important to consider effective brightness of NIR FPs in cells, in addition...binding of endogenous BV.  When imaging NIR FPs, consider using light sources that produce enough light ...NIR. Usually, mercury lamps are suboptimal, metal halide lamps are better, and xenon lamps are the best ...apparent cellular brightness than previously reported widely used dimeric iRFPs (Table 1). In general, the monomeric...O.S. et al. Single-domain near-infrared proteins provide a scaffold for antigen-dependent fluorescent nanobodies...

  8. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...educational science videos is tough. Is the content accurate? Are we conveying complex ideas in the easiest... production standpoint, it wasn’t ideal for our Lab Protocol videos. It’s really nice to have a scientist...perfect height (Fig. 2). We recorded video using a simple, external video recorder and a mic clipped to her...pulled it off! Check out the PCR Primer video! Learn: Producing videos like this would prove to be difficult..., and as a video control center.   Cycle 2: Zooming Forward Design: We produced a video celebrating... cues. We had two options: Film outside at a distance, or film inside with one person in a room. I wouldn...protocol videos?   Figure 4: The remote directing setup, complete with wireless video receiver,...

  9. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    ...very excited to debut our brand new Addgene Videos page! Videos are a powerful tool for communicating scientific... first video, featuring Outreach Scientist Jessica Welch, can be found below: How-to videos Our How-To...Videos can save researchers time by quickly relaying key points about an unfamiliar topic or lab process... page, we have neatly organized the educational video content from our website, blog, and YouTube channel...questions you might have about Addgene. The page is divided into three main sections: Protocols To complement...written protocol materials, we have also created video protocols.  These feature step-by-step instructions...various lab processes.  Our most recent protocol video detailing Streaking Plates for Single Bacterial ...

  10. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    ...plasmid rap video for us (If you’re new to plasmids, we highly recommend checking out the video). Tom has...Beautifully filmed video about colony collapse disorder.     5. Plasmid Rap Plasmid rap video made with support...has made many more Science rap videos to teach students around the globe and is pushing SciComm further...Rock Anthem One of Tom's earliest and most popular videos. Describes fossil rocks and their importance in... Check out Our Protocol Pages for New Protocol Videos View Our Molecular Biology Reference Pages ...

  11. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...reverse transcriptase) together with a prime editing guide RNA (pegRNA). The pegRNA consists of a target-complementary...on the website or offline via GitHub. The model provides users with different designs of pegRNAs, ranked...and test them in their labs. Figure 2: Guidelines to predict pegRNA efficiencies with pridict.it...computational prediction models. In addition, we provide options for nicking gRNAs (used for PE3 and PE5...self-targeting screens such as PRIDICT/PRIDICT2.0 provide valuable insights into pegRNA editing efficiencies...Brinkman, E. K., Leemans, C., Vergara, X., van der Weide, R. H., Morris, B., van Schaik, T., Manzo, S. G....Addgene.org CRISPR: PRIME Edit Collection The CRISPR Guide Empty gRNA Expression Vectors...

  12. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...protein of interest, these tools provide an easier means to identify targets through fluorescent labeling...Lavis, L. D. (2022). Caveat fluorophore: an insiders' guide to small-molecule fluorescent labels. Nature...interiors to many inquisitive scientists. Seeing inside these specimens has offered illumination on biochemical...drug development (Grimm & Lavis, 2022). With the wide breadth of fluorescent tools that have been developed...will outline some fundamental characteristics to consider when selecting a dye or ligand to make your selection...protein, enabling the labeling of protein substrates/peptides and antibodies. Example applications of this include... is bound through green light emission. Key considerations in selecting fluorescent dyes/ligands Effects...

  13. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    ...questions, data, and conclusions should be clearly identified and interpreted in less than a couple of minutes...Tips for designing your poster Check conference guidelines To begin, you should always follow conference...poster at all times, so other participants can identify you. You may also wish to make creative use of...three, or four column style, and in all cases the idea is the same; the reader will navigate the poster... to low, some of the poster will naturally sit outside of the reader’s field of view because of its length...to read. The Thompson Rivers University Library provides useful tips on how to craft this type of poster...doctoral student at Michigan State University. The idea is that the main research finding is written across...

  14. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    .... The video abstracts provide a brief introduction to the research and, after watching the video to determine...platform that provides links to the videos, keeping the copyright and control over the videos with the researcher...others. Improving science communication through video     Video abstracts about research, especially published...view count and videos published, YouTube reported a total of 865,995 views for the 926 video abstracts identified...attention for their work, a video abstract may be a useful tool for turning video views into online article...few years, I decided to look at what online tools people generally like to use to share ideas – with the... online video market ever since. The take home message of all of this appears to be that video is the ...

  15. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ... CRISPR RNA Scaffolds." Adam has also created a video to help scientists visualize the concepts discussed...endonuclease activity coupled with a customizable single guide RNA (sgRNA) that bears complementarity to a 20bp...RNA (scRNA) will bind to a PAM-adjacent target nucleotide sequence then subsequently recruit a transcriptional...orthogonal dCas9 protein that will still recognize guide RNA but with a different dCas9 binding module, alternative... molecular microbiologist. Summary  The single guide RNA in the CRISPRi system can be repurposed to include... Qi, L. S. et al. Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene ...

  16. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ...your machine!  Finally, many NanoDrop systems now provide full-spectrum analysis, rather than just the absorbances...Full-spectrum analysis allows the systems to better identify contaminants and to calculate corrected concentrations...critical interpretation of the results.  If you see evidence of contamination, you can try re-purifying the...these long enough?  There are, of course, more considerations and other approaches for measuring DNA quantity...use and check out some of the references we have provided below. And if you do have trouble with contamination...1ea8aec3bfa24543a28fcaea25986514.ashx. Koetsier G and Cantor E. A Practical Guide to Analyzing Nucleic Acid Concentration and Purity...

  17. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    ...things to consider: cost, time, equipment, and expected DNA concentration. It’s important to consider the advantages...DNA sample.  These absorbance measures give you an idea of the concentration of your DNA prep and whether...A280 ratio is used as an indicator of DNA purity. Ideally, this number should be between 1.8 and 2.0. The...containing a DNA intercalating dye (ex: ethidium bromide) and choosing a DNA ladder with known concentrations...

  18. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...have allowed scientists to easily perform genome-wide screens to effectively and efficiently investigate...libraries contain many sgRNAs per gene to increase confidence in the genetic screen. However, there are benefits...utilize only a few sgRNAs per gene. These libraries provide more information while using less resources and... the Broad Institute have developed human genome-wide CRISPRko (knock out), CRISPRi (interference), and... sgRNAs that were designed based on the rules identified in Doench et al., 2014 to predict sgRNA activity...scientists examined approximately 1,900 sgRNAs and identified sequence features and adjustments that improved...performance. The Root and Doench lab’s optimized genome-wide CRISPRko, CRISPRi, and CRISPRa libraries have been...

  19. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...FLEx Vectors Important Considerations When Using AAVs Three Key Considerations For Precise Neuronal Targeting...reproducibility of the data and can instill greater confidence when interpreting the outcome.    References...Sequencing and Analysis Platform to Assess the Identity of Recombinant Adeno-Associated Viral Preparations...

  20. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...gene function. In this final installment, I’ll provide a brief introduction as to how you can engineer...Addgene also has a number of insect vectors to consider. An easy way to see what is available is to browse...transgenic fly There are a few important points to consider when choosing the vector you’d like to use to ...pathogens and their hosts. It’s not always clear which side he roots for. You can try to convince him to use...
Showing: 161 - 180 of 1106 results