We narrowed to 1,104 results for: Ide

...majority of cells failing to develop the desired identity. The development of single cell technologies, ... RNA-seq (scRNA-seq), now allows scientists to identify the gene expression patterns unique to the few...Morris Lab at Washington University in St. Louis provides a combinatorial indexing strategy that simultaneously...simultaneously tracks clonal history and cell identity at the single-cell level, thus linking ancestral gene...Biddy et al., 2018). Tracking lineage and cell identity at the single-cell level with CellTag CellTagging... region. The variable region acts as a unique identifier for individual cells. Each cell is labeled with.... The fixed region acts as a library-specific identifier and allows cells to be tagged with different ...

...I led. It’s divided into the key takeaway themes and customer pain points we’ve identified. I can’t wait...software product management, I confess that I had no idea what software product management was either. Coming...

...developers of many of these probes, we decided to write this quick guide to help researchers choose the probe...supplied (Baloban et al., 2017).  It is important to consider effective brightness of NIR FPs in cells, in addition...binding of endogenous BV.  When imaging NIR FPs, consider using light sources that produce enough light ...NIR. Usually, mercury lamps are suboptimal, metal halide lamps are better, and xenon lamps are the best ...apparent cellular brightness than previously reported widely used dimeric iRFPs (Table 1). In general, the monomeric...O.S. et al. Single-domain near-infrared proteins provide a scaffold for antigen-dependent fluorescent nanobodies...

...but it isn’t just for lab specimens — there is evidence that lightning can also promote gene transfer ...While these plasmids can be dangerous, they also provide researchers with new means to deliver DNA and other...resistance to toxic substances like heavy metals or pesticides, enabling their hosts to survive where others... T. M., & Simonet, P. (2001). Laboratory-scale evidence for lightning-mediated gene transfer in soil. ...

...educational science videos is tough. Is the content accurate? Are we conveying complex ideas in the easiest... production standpoint, it wasn’t ideal for our Lab Protocol videos. It’s really nice to have a scientist...perfect height (Fig. 2). We recorded video using a simple, external video recorder and a mic clipped to her...pulled it off! Check out the PCR Primer video! Learn: Producing videos like this would prove to be difficult..., and as a video control center.   Cycle 2: Zooming Forward Design: We produced a video celebrating... cues. We had two options: Film outside at a distance, or film inside with one person in a room. I wouldn...protocol videos?   Figure 4: The remote directing setup, complete with wireless video receiver,...

...journal club. Sorting out the logistics Once you decide on the topic, there are a number of administrative...notice for the first meeting so that people can set aside time in their schedules and read the paper (PS: ... on single papers could be more important.  Any slides, presentations, recordings, and other materials... ensure that the journal club is useful for the widest audience, you should get feedback from those who...everyone to offer thoughts you may not have even considered. These questions should be tailored to the group...priority for the participants. Perhaps people feel confident in their own knowledge of the field. Perhaps interest... not actively participating, it may be time to consider ending the meeting. This is a common failing in...

... it! Further Reading and Outside Resources http://www.businessinsider.com/how-to-get-the-most-out-of-job-fairs...your lab, right? Wrong! While it is clearly a good idea to be diligent in your research, remember that you...themselves, resources are often scarce and difficult to identify. So what specifically should you do? What follows... elaborated on later. As for what job fairs to consider, some of the best options are the Naturejobs Career...most relevant experience at the top. You may also consider visiting any of the employer’s career pages to... apply for the position. This post on Business Insider nicely summarizes good job fair practice. Career...and other online career resources Once you’ve identified places you’d like to work, get online and take...

...questions, data, and conclusions should be clearly identified and interpreted in less than a couple of minutes...Tips for designing your poster Check conference guidelines To begin, you should always follow conference...poster at all times, so other participants can identify you. You may also wish to make creative use of...three, or four column style, and in all cases the idea is the same; the reader will navigate the poster... to low, some of the poster will naturally sit outside of the reader’s field of view because of its length...to read. The Thompson Rivers University Library provides useful tips on how to craft this type of poster...doctoral student at Michigan State University. The idea is that the main research finding is written across...

...protein of interest, these tools provide an easier means to identify targets through fluorescent labeling...Lavis, L. D. (2022). Caveat fluorophore: an insiders' guide to small-molecule fluorescent labels. Nature...interiors to many inquisitive scientists. Seeing inside these specimens has offered illumination on biochemical...drug development (Grimm & Lavis, 2022). With the wide breadth of fluorescent tools that have been developed...will outline some fundamental characteristics to consider when selecting a dye or ligand to make your selection...protein, enabling the labeling of protein substrates/peptides and antibodies. Example applications of this include... is bound through green light emission. Key considerations in selecting fluorescent dyes/ligands Effects...

...reverse transcriptase) together with a prime editing guide RNA (pegRNA). The pegRNA consists of a target-complementary...on the website or offline via GitHub. The model provides users with different designs of pegRNAs, ranked...and test them in their labs. Figure 2: Guidelines to predict pegRNA efficiencies with pridict.it...computational prediction models. In addition, we provide options for nicking gRNAs (used for PE3 and PE5...self-targeting screens such as PRIDICT/PRIDICT2.0 provide valuable insights into pegRNA editing efficiencies...Brinkman, E. K., Leemans, C., Vergara, X., van der Weide, R. H., Morris, B., van Schaik, T., Manzo, S. G....Addgene.org CRISPR: PRIME Edit Collection The CRISPR Guide Empty gRNA Expression Vectors...

.... The video abstracts provide a brief introduction to the research and, after watching the video to determine...platform that provides links to the videos, keeping the copyright and control over the videos with the researcher...others. Improving science communication through video     Video abstracts about research, especially published...view count and videos published, YouTube reported a total of 865,995 views for the 926 video abstracts identified...attention for their work, a video abstract may be a useful tool for turning video views into online article...few years, I decided to look at what online tools people generally like to use to share ideas – with the... online video market ever since. The take home message of all of this appears to be that video is the ...

... CRISPR RNA Scaffolds." Adam has also created a video to help scientists visualize the concepts discussed...endonuclease activity coupled with a customizable single guide RNA (sgRNA) that bears complementarity to a 20bp...RNA (scRNA) will bind to a PAM-adjacent target nucleotide sequence then subsequently recruit a transcriptional...orthogonal dCas9 protein that will still recognize guide RNA but with a different dCas9 binding module, alternative... molecular microbiologist. Summary  The single guide RNA in the CRISPRi system can be repurposed to include... Qi, L. S. et al. Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene ...

...have allowed scientists to easily perform genome-wide screens to effectively and efficiently investigate...libraries contain many sgRNAs per gene to increase confidence in the genetic screen. However, there are benefits...utilize only a few sgRNAs per gene. These libraries provide more information while using less resources and... the Broad Institute have developed human genome-wide CRISPRko (knock out), CRISPRi (interference), and... sgRNAs that were designed based on the rules identified in Doench et al., 2014 to predict sgRNA activity...scientists examined approximately 1,900 sgRNAs and identified sequence features and adjustments that improved...performance. The Root and Doench lab’s optimized genome-wide CRISPRko, CRISPRi, and CRISPRa libraries have been...

...gene function. In this final installment, I’ll provide a brief introduction as to how you can engineer...Addgene also has a number of insect vectors to consider. An easy way to see what is available is to browse...transgenic fly There are a few important points to consider when choosing the vector you’d like to use to ...pathogens and their hosts. It’s not always clear which side he roots for. You can try to convince him to use...

...deposition of the fertilized egg by its mother. Inside the pupal case, the larva will metamorphose into...light in coloration and can look very plump. On the side of their abdomen will be a dark spot. This is meconium...visible through their translucent bodies. This is the ideal stage to select for females because you can be certain...have an allele that causes the hairs on the dorsal side of its thorax to look like stubble (sb). For a better...search box in the upper right hand corner. They have video tutorials under the help tab like this one to get...pathogens and their hosts. It’s not always clear which side he roots for. You can try to convince him to use...

...things to consider: cost, time, equipment, and expected DNA concentration. It’s important to consider the advantages...DNA sample.  These absorbance measures give you an idea of the concentration of your DNA prep and whether...A280 ratio is used as an indicator of DNA purity. Ideally, this number should be between 1.8 and 2.0. The...containing a DNA intercalating dye (ex: ethidium bromide) and choosing a DNA ladder with known concentrations...

...FLEx Vectors Important Considerations When Using AAVs Three Key Considerations For Precise Neuronal Targeting...reproducibility of the data and can instill greater confidence when interpreting the outcome.    References...Sequencing and Analysis Platform to Assess the Identity of Recombinant Adeno-Associated Viral Preparations...

...your machine!  Finally, many NanoDrop systems now provide full-spectrum analysis, rather than just the absorbances...Full-spectrum analysis allows the systems to better identify contaminants and to calculate corrected concentrations...critical interpretation of the results.  If you see evidence of contamination, you can try re-purifying the...these long enough?  There are, of course, more considerations and other approaches for measuring DNA quantity...use and check out some of the references we have provided below. And if you do have trouble with contamination...1ea8aec3bfa24543a28fcaea25986514.ashx. Koetsier G and Cantor E. A Practical Guide to Analyzing Nucleic Acid Concentration and Purity...

...the Dark Side." --Darth Vader in "Return of the Jedi" While Vader was referring to the evil side of a mystical...and rewarding. But, what if an FP fusion with a resident endoplasmic reticulum (ER) protein instead localizes...Previously, we have described a number of practical considerations that should be taken into account when deciding...must therefore determine your cloning strategy and decide whether you will create an N- or C-terminal fusion...needs. Equally important, before cloning you must consider whether and how well your FP will function in ...secreted into the extracellular milieu. Many of the resident proteins within these compartments undergo significant...post-translational modifications including glycosylation, disulfide bond formation, and proteolytic cleavage. FPs ...

.... Watch the following video to learn Daniel and Matthew's procedures. The video details how to transfect...Harvard Medical School. Addgene is proud to present a video reprint of the CRISPR article "Generation of Genomic...the Journal of Visualized Experiments (JOVE). The video publication by Stuart Orkin and Daniel Bauer's labs.... Biallelically disrupted cells can be easily identified. Even in the case of clones with monoallelic ...for biallelic deletion clones:    Like the video? The Orkin and Bauer lab's related paper describes... as their techniques for creating deletions, identifying these deletions, and finally characterization...