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...very excited to debut our brand new Addgene Videos page! Videos are a powerful tool for communicating scientific... first video, featuring Outreach Scientist Jessica Welch, can be found below: How-to videos Our How-To...Videos can save researchers time by quickly relaying key points about an unfamiliar topic or lab process... page, we have neatly organized the educational video content from our website, blog, and YouTube channel...questions you might have about Addgene. The page is divided into three main sections: Protocols To complement...written protocol materials, we have also created video protocols.  These feature step-by-step instructions...various lab processes.  Our most recent protocol video detailing Streaking Plates for Single Bacterial ...

...but it isn’t just for lab specimens — there is evidence that lightning can also promote gene transfer ...While these plasmids can be dangerous, they also provide researchers with new means to deliver DNA and other...resistance to toxic substances like heavy metals or pesticides, enabling their hosts to survive where others... T. M., & Simonet, P. (2001). Laboratory-scale evidence for lightning-mediated gene transfer in soil. ...

...I led. It’s divided into the key takeaway themes and customer pain points we’ve identified. I can’t wait...software product management, I confess that I had no idea what software product management was either. Coming...

...journal club. Sorting out the logistics Once you decide on the topic, there are a number of administrative...notice for the first meeting so that people can set aside time in their schedules and read the paper (PS: ... on single papers could be more important.  Any slides, presentations, recordings, and other materials... ensure that the journal club is useful for the widest audience, you should get feedback from those who...everyone to offer thoughts you may not have even considered. These questions should be tailored to the group...priority for the participants. Perhaps people feel confident in their own knowledge of the field. Perhaps interest... not actively participating, it may be time to consider ending the meeting. This is a common failing in...

...plasmid rap video for us (If you’re new to plasmids, we highly recommend checking out the video). Tom has...Beautifully filmed video about colony collapse disorder.     5. Plasmid Rap Plasmid rap video made with support...has made many more Science rap videos to teach students around the globe and is pushing SciComm further...Rock Anthem One of Tom's earliest and most popular videos. Describes fossil rocks and their importance in... Check out Our Protocol Pages for New Protocol Videos View Our Molecular Biology Reference Pages ...

...educational science videos is tough. Is the content accurate? Are we conveying complex ideas in the easiest... production standpoint, it wasn’t ideal for our Lab Protocol videos. It’s really nice to have a scientist...perfect height (Fig. 2). We recorded video using a simple, external video recorder and a mic clipped to her...pulled it off! Check out the PCR Primer video! Learn: Producing videos like this would prove to be difficult..., and as a video control center.   Cycle 2: Zooming Forward Design: We produced a video celebrating... cues. We had two options: Film outside at a distance, or film inside with one person in a room. I wouldn...protocol videos?   Figure 4: The remote directing setup, complete with wireless video receiver,...

...database of common features, and devising rules for identifying a feature even when the match is imperfect. SnapGene's...catalog every variant of a feature. Instead, we identified common variants, and then crafted a detection...reasonable rule is to require at least 96% sequence identity when detecting a reference feature. For a coding...match even when the DNA sequence has changed. Identifying plasmid control elements Coding sequence features...original literature, some of it decades old, to provide reliable feature annotations. Even with these extensive...that have different properties. To prevent misidentification, we augment the database with new fluorescent...protein variants. The future of DNA feature identification What’s next for common features? We plan to...

...questions, data, and conclusions should be clearly identified and interpreted in less than a couple of minutes...Tips for designing your poster Check conference guidelines To begin, you should always follow conference...poster at all times, so other participants can identify you. You may also wish to make creative use of...three, or four column style, and in all cases the idea is the same; the reader will navigate the poster... to low, some of the poster will naturally sit outside of the reader’s field of view because of its length...to read. The Thompson Rivers University Library provides useful tips on how to craft this type of poster...doctoral student at Michigan State University. The idea is that the main research finding is written across...

...to specific genomic targets. Hybrid guides are oligonucleotides with a mixture of DNA and RNA bases. ...you want your hybrid guide to be effective. Which bases can be substituted? A guide RNA has many parts....reduced, but hybrid guides are unlikely to impact other kinds of unintended edits, like guide-independent deamination...The genome-editing tool CRISPR is famously RNA-guided... except when it’s not. Turns out, carefully designed...long as specific protein-contacting bases were avoided (Yin et al., 2018). Figure 1: The seed, ...reduced off-target effects, this means the hybrid guides are more specific. Reduced bystander editing by... the target base. It’s not yet clear why hybrid guides reduce bystander editing, and other kinds of base...

...protein of interest, these tools provide an easier means to identify targets through fluorescent labeling...Lavis, L. D. (2022). Caveat fluorophore: an insiders' guide to small-molecule fluorescent labels. Nature...interiors to many inquisitive scientists. Seeing inside these specimens has offered illumination on biochemical...drug development (Grimm & Lavis, 2022). With the wide breadth of fluorescent tools that have been developed...will outline some fundamental characteristics to consider when selecting a dye or ligand to make your selection...protein, enabling the labeling of protein substrates/peptides and antibodies. Example applications of this include... is bound through green light emission. Key considerations in selecting fluorescent dyes/ligands Effects...

...reverse transcriptase) together with a prime editing guide RNA (pegRNA). The pegRNA consists of a target-complementary...on the website or offline via GitHub. The model provides users with different designs of pegRNAs, ranked...and test them in their labs. Figure 2: Guidelines to predict pegRNA efficiencies with pridict.it...computational prediction models. In addition, we provide options for nicking gRNAs (used for PE3 and PE5...self-targeting screens such as PRIDICT/PRIDICT2.0 provide valuable insights into pegRNA editing efficiencies...Brinkman, E. K., Leemans, C., Vergara, X., van der Weide, R. H., Morris, B., van Schaik, T., Manzo, S. G....Addgene.org CRISPR: PRIME Edit Collection The CRISPR Guide Empty gRNA Expression Vectors...

.... The video abstracts provide a brief introduction to the research and, after watching the video to determine...platform that provides links to the videos, keeping the copyright and control over the videos with the researcher...others. Improving science communication through video     Video abstracts about research, especially published...view count and videos published, YouTube reported a total of 865,995 views for the 926 video abstracts identified...attention for their work, a video abstract may be a useful tool for turning video views into online article...few years, I decided to look at what online tools people generally like to use to share ideas – with the... online video market ever since. The take home message of all of this appears to be that video is the ...

... CRISPR RNA Scaffolds." Adam has also created a video to help scientists visualize the concepts discussed...endonuclease activity coupled with a customizable single guide RNA (sgRNA) that bears complementarity to a 20bp...RNA (scRNA) will bind to a PAM-adjacent target nucleotide sequence then subsequently recruit a transcriptional...orthogonal dCas9 protein that will still recognize guide RNA but with a different dCas9 binding module, alternative... molecular microbiologist. Summary  The single guide RNA in the CRISPRi system can be repurposed to include... Qi, L. S. et al. Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene ...

...have allowed scientists to easily perform genome-wide screens to effectively and efficiently investigate...libraries contain many sgRNAs per gene to increase confidence in the genetic screen. However, there are benefits...utilize only a few sgRNAs per gene. These libraries provide more information while using less resources and... the Broad Institute have developed human genome-wide CRISPRko (knock out), CRISPRi (interference), and... sgRNAs that were designed based on the rules identified in Doench et al., 2014 to predict sgRNA activity...scientists examined approximately 1,900 sgRNAs and identified sequence features and adjustments that improved...performance. The Root and Doench lab’s optimized genome-wide CRISPRko, CRISPRi, and CRISPRa libraries have been...

...FLEx Vectors Important Considerations When Using AAVs Three Key Considerations For Precise Neuronal Targeting...reproducibility of the data and can instill greater confidence when interpreting the outcome.    References...Sequencing and Analysis Platform to Assess the Identity of Recombinant Adeno-Associated Viral Preparations...

...gene function. In this final installment, I’ll provide a brief introduction as to how you can engineer...Addgene also has a number of insect vectors to consider. An easy way to see what is available is to browse...transgenic fly There are a few important points to consider when choosing the vector you’d like to use to ...pathogens and their hosts. It’s not always clear which side he roots for. You can try to convince him to use...

...deposition of the fertilized egg by its mother. Inside the pupal case, the larva will metamorphose into...light in coloration and can look very plump. On the side of their abdomen will be a dark spot. This is meconium...visible through their translucent bodies. This is the ideal stage to select for females because you can be certain...have an allele that causes the hairs on the dorsal side of its thorax to look like stubble (sb). For a better...search box in the upper right hand corner. They have video tutorials under the help tab like this one to get...pathogens and their hosts. It’s not always clear which side he roots for. You can try to convince him to use...

...your machine!  Finally, many NanoDrop systems now provide full-spectrum analysis, rather than just the absorbances...Full-spectrum analysis allows the systems to better identify contaminants and to calculate corrected concentrations...critical interpretation of the results.  If you see evidence of contamination, you can try re-purifying the...these long enough?  There are, of course, more considerations and other approaches for measuring DNA quantity...use and check out some of the references we have provided below. And if you do have trouble with contamination...1ea8aec3bfa24543a28fcaea25986514.ashx. Koetsier G and Cantor E. A Practical Guide to Analyzing Nucleic Acid Concentration and Purity...

...things to consider: cost, time, equipment, and expected DNA concentration. It’s important to consider the advantages...DNA sample.  These absorbance measures give you an idea of the concentration of your DNA prep and whether...A280 ratio is used as an indicator of DNA purity. Ideally, this number should be between 1.8 and 2.0. The...containing a DNA intercalating dye (ex: ethidium bromide) and choosing a DNA ladder with known concentrations...

...the Dark Side." --Darth Vader in "Return of the Jedi" While Vader was referring to the evil side of a mystical...and rewarding. But, what if an FP fusion with a resident endoplasmic reticulum (ER) protein instead localizes...Previously, we have described a number of practical considerations that should be taken into account when deciding...must therefore determine your cloning strategy and decide whether you will create an N- or C-terminal fusion...needs. Equally important, before cloning you must consider whether and how well your FP will function in ...secreted into the extracellular milieu. Many of the resident proteins within these compartments undergo significant...post-translational modifications including glycosylation, disulfide bond formation, and proteolytic cleavage. FPs ...