Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 1,018 results for: REN;

Showing: 161 - 180 of 1018 results

  2. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...Sample Data Selection of E.coli on LB-agar using different concentrations of carbenicillin plated over-agar...over-agar Plate shows a lawn of E. coli and no apparent selection. 150 µL of 1 mg/mL Carbenicillin plated...

  3. Water Bath Protocol

    Type
    Protocol
    ... biology, and thaw cell lines. There are many different types of water baths that exist as scientists ...scientists work with tubes and bottles of different sizes in the lab. Some water baths contain individual slots...

  4. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ... of the four levels is to distinguish between different types of hazards and to provide protective measures...measures for each level, as each level has different safety requirements. BSL-1 is designated for those...

  5. Lentivirus Production

    Type
    Protocol
    ...21-040-CV (cations can affect the attachment of adherent cells) 0.45 μm polyethersulfone filter, Nalgene...and 5 mL of glutaGRO. Store at 4 °C. Pro-Tip Different brands and lots of FBS can promote or inhibit ...

  7. Kit Free RNA Extraction

    Type
    Protocol
    ...Purification Agarose Gel Purification Molecular Biology Reference Introduction This protocol describes how to extract...ThermoFisher Scientific . Figure 1: A diagram of the different steps in RNA extraction. Before Starting RNA is...

  8. Deep Dive: qPCR

    Type
    Blog Post
    ...melting profiles (Dwight, 2011).  Reference Genes  Choosing a set of reference genes deserves more attention...attention than it usually receives. Different reference genes have shown to have as much as sixfold variation...of Connect Four! Image created in BioRender.   Passive Reference Dyes Whatever your pipetting method...occur. A passive reference dye, such as ROX, can help normalize for that. Passive reference dyes are inert...Liu and Saint No Raw Data Reference and target genes can have different efficacies DART-PCR No ...will soon become a qPCR master. References and Resources  References Biassoni R; Raso A. (eds.), Quantitative...mRNA, which can be used as a proxy measure for differences in protein expression. (If you're just getting...

  10. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...heterogeneous population, cells behaving differently (e.g. different growth properties, migration abilities...targeting all differentially expressed genes that are known drug targets, or all differentially expressed ... by choosing which “Reference Library” you’d like to use in your screen. Currently, eleven published libraries...best gRNAs by the depositing lab. The different labs use different algorithms to calculate which would ...favorite Reference Library, for example related to strategies beyond SpCas9, or species not currently included...were not found in the reference library. It’s not uncommon that genes have different names, and the software...generation sequencing to identify which gRNAs are differentially expressed in the separate groups and, as a ...

  12. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...For CD365, they examined two different antibody clones produced by different vendors. The specific epitope... panels, where different manufacturers may have the same clone available with different conjugates. Summary...commercial antibody manufacturers. References and resources References Ayoubi, R., Ryan, J., Biddle, M. ...include a discussion of the Human Leucocyte Differentiation Antigens (HCDM) workshop approach, which focuses...one size fits all. The approaches used will be different depending on the sample and target type. To start...an approach that prioritizes more reproducible/renewable reagents, such as recombinant or hybridoma-derived...(e.g. DepMap) – you’ll need to ensure that the parental cell lines of your chosen KO line are likely to...

  13. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...most organisms on Earth, but different organisms have different preferences for the codons they use to ...have different codon usage biases? The reasons for varied codon preferences among organisms aren’t completely...poorly charged tRNAs, different regions of the protein may be translated at different rates. The ribosome...Organisms often need to express genes at different levels under different conditions. With varied codon usage...constraints have molded which codons are used preferentially in which organisms - organisms have codon usage...your organism of choice. Unfortunately, codon preferences make it so you cannot choose among the possible... evolutionary constraints that lead to these preferences and what can we do about them? Read on to find...

  14. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ... directed differentiation To understand how each human TF can contribute to differentiation, the Zhang... scientist at [email protected]! References and Resources  References Joung J. et al., A transcription...patterns determine everything from stem cell differentiation fate to tumor suppression (and most everything...construct. Cells were cultured following a differentiation protocol before performing single-cell RNA ...embryonic stem cells. Transduced stem cells were differentiated and single cell RNA-seq was performed to map...    This atlas linked TF expression to differentiation outcomes as well as global transcriptional ...program changes from this screen successfully differentiated cells resembling all three germ layers (ectoderm...

  15. Lentiviral Vector Guide

    Type
    Guide
    ...packaging genes. However, they are different viruses and require different isoforms of these packaging components...genes. From an experimental standpoint, the main difference between lentiviruses and gamma-retroviruses is...: Wild-type lentivirus genome. Created with BioRender.com. All retroviruses use the packaging genes gag...systems, due to a wide range of infectivity for different cell types (known as tropism). Lentiviral vectors...safety. These improvements have been divided into different generations. Lentiviral Generations First-generation...While the viral components are still separated on different plasmids, the viral genome remains largely intact...First-generation lentiviral plasmids. Created with BioRender.com. Second-generation Second-generation lentiviral...

  16. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...reflect massive differences in protein abundance. Validating the assumptions for different loading controls...detection range be linear! Resources and References References Aldridge, G. M., Podrebarac, D. M., Greenough... that lanes are loaded evenly. Created with BioRender.com. Loading controls are even more important...entire blot. So how can you accurately compare different lanes? In relative quantification, the POI bands... the two conditions are equal. Created with BioRender.com. This kind of normalization is a nearly ...with separate controls (green). Created with BioRender.com. Researchers often rely on assumptions that...normalize your results, you are assuming that all differences in the loading control are due to loading and...

  17. Optogenetics Guide

    Type
    Guide
    ...reduces photocurrent amplitude 490 ChR/T159C T159C mutation; displays increased photocurrents 470 SFO/...outside of the brain, rendering the experimental procedure less invasive. Different activation wavelengths...Introduction Microbial Opsins Optical Switches References Introduction The field of optogenetics integrates...naturally occurring microbial opsins that respond to different wavelengths of light, like blue or yellow light...light. These various opsins also initiate different electrochemical responses, such as nonspecific cation...optimization, and chimeric fusion of domains from two different ChRs. These ChR variants still function as light-gated...neuron. Feature enhancements include: Increased photocurrent amplitude Examples: ChR2(H134R), C1V1(t/t), ...

  18. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...organism. Output diversity: Different tools provide results to the user in different formats. This can have...the piñata beating party goers are altogether different. They are filled with blind anticipation. Their...spill to the ground. There is something akin to a frenzy as the crowd tries to weigh and determine the value...response; and, it is the stage I think we are currently at in the CRISPR software space. The CRISPR research...year.   Breaking down the barriers: My view on current sgRNA design tools The goal of this post is to ... sgRNA and represents the lion’s share of the currently available tools (we will focus on these tools ...required to fully evaluate off-targeting, but currently no software has been designed for this purpose...

  19. Plan Your Experiment

    Type
    Guide
    ...many different Cas proteins available, and researchers can adapt these experiments for different organisms...experiment. Created with BioRender.com. Select Your Desired Genetic Manipulation Different genetic manipulations...Activation/interference Needs good primer design Western blot Knockouts Activation/interference Needs a ...Expression and Delivery Validate Genetic Edit References CRISPR ( C lustered R egularly I nterspaced S...have expanded the CRISPR toolbox to include many different types of edits, including point mutations, base...manipulations require different CRISPR components. Selecting the genetic manipulation you plan to perform will...Base editor (dCas9 or Cas9 nickase fusions with different base editing proteins) Many base editors fused...

  20. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ... with biorender.com.   Of course, this is quite easy to do on a plot generated in Biorender! While...with that…and happy flowing!    References and resources  References Alyamani, A., Kalamegam, G., Ahmed...read from Marker A and Marker B. Created with biorender.com.   The goal of flow cytometry is to use ...usually tell where this point is by looking at the different populations. In the case of Figure 2, one population...and one population that is B-. Created with biorender.com.   If it’s hard to conceptualize where the...   Figure 3: It's often easier to identify different populations by imagining (or drawing) a quadrant...population and everything outside the gate is a different population.  Gating and plot orders  As the gates...
Showing: 161 - 180 of 1018 results