...Pipet back and forth to resuspend each pellet completely. Combine resuspended pellets and keep on ice....Pipet back and forth to resuspend each pellet completely. Combine all resuspended cell pellets and sonicate...containing the resuspended virus from step 12 above. Add 50 units of benzonase per mL of viral suspension (you ...cells after 30 passages. Procedure Trypsinize and resuspend the HEK293T cells from 2 x T-175 flasks. Cells...vigorously multiple times to obtain a single cell suspension (no clumps of cells). Pool cells from 2 x T-175... Return to incubator for 48 h. Trypsinize and resuspend cells from the CS2. Cells should be at ~80% confluence...forth or shake vigorously to obtain a single cell suspension (no clumps). Count cells using a hemocytometer...

...tissues, extraction of RNA, precipitation, and resuspension. This protocol provides two options: using Solution...RNA in the bottom of the tube. Wash the RNA by resuspending the pellet in 0.5–1 ml of 75% Ethanol and vortex...important to not let the pellet get too dry before resuspending, as this affects the solubility of the RNA. ... but while the white pellet is still visible. Resuspend RNA pellet in RNase-free water or TE. Quantify...

...microcentrifuge tube. Vortex the cell suspension. Transfer 10 µL of cell suspension into the microfuge tube containing...culture. This protocol describes how to transfect suspension HEK293 cells with recombinant antibody plasmids... 5 mL pipette, transfer 0.5 mL of HEK293 cell suspension into a clean microcentrifuge tube. Pro-Tip Cells...Cells settle quickly and need to be resuspended before sampling. Gently swirl the flask 5–10 times before...Pipette 10 times to mix. Load 10 µL of the cell suspension/trypan blue mix into a cell counting chamber....

... media and 11.1 µg/mL Polybrene. Mix the cell suspension well before seeding. Mix each well with a 1 mL...by incubating with 200 µL TrypLE for 1–2 min. Resuspend cells in 500 µL DMEM complete and transfer to ...the master mix to each PCR tube. Be careful to dispense to the bottom of the tube without collecting drops...45° angle. Count to 20 while slowly and gently dispensing the droplets down the side of the tube. Place...

...of reagent that will be resuspended in a small volume (e.g. 0.02 g to resuspend in 1 mL), you may place...

...5 min at 100 x g . Gently remove supernatant. Resuspend cell pellet in 1 mL of 1X PBS and transfer to ...min at 100 x g . Carefully remove supernatant. Resuspend cell pellet in appropriate volume of cold lysis...

...the master mix to each PCR tube. Be careful to dispense to the bottom of the tube without collecting drops...45° angle. Count to 20 while slowly and gently dispensing the droplets down the side of the tube. Place...

...for all plates. Use two 10 mL pipettes (one for dispensing cold LB and one for removing LB containing bacteria...cells are subject to lysis by freeze-thaw if not suspended in cryoprotectant like Glycerol or DMSO solutions...

...spectrophotometer using the solution the DNA is resuspended in, but with no DNA added. 'Blanking' measures...

...Plate 50 µL of transformed E. coli /rescue media suspension onto the agar and gently spread over the surface...

...protocol. Anneal oligos The oligos should be resuspended in annealing buffer (10mM Tris, pH7.5-8.0, 50mM...

...selecting the correct pipettor and pipette tip, how to dispense liquids, and how to handle the pipette Watch the...

... pipetting or inverting. Aliquot 2 mL of cell suspension into each well of the 6-well dish. Incubate the...

...gently collect the cells by centrifugation and resuspend in a smaller volume of LB so that there isn't ...

...pipetting or inverting. Aliquot 1.35 mL of the cell suspension into each well of the 6-well dish directly on...

...pipetting or inverting the tube. Aliquot 1 mL of cell suspension (i.e., 50,000 cells) into each well of the 6-...

... Use a reservoir and a multichannel pipette to dispense the master mix into the wells. Reagent Amount ...

...during the autoclave process if your sample has spent at least 10 min at 121 ℃. Use lab tape to label ...

...optimized for adherent cells but can be modified for suspension cells. The number of monoclonal lines generated...

...#80030-956. Use at 100 μg/mL. C.2 Annealing Oligos Resuspend oligos in ddH2O to a concentration of 20 μM, then...